Comparative HPTLC analysis of stem and leaf of Achyranthes coynei with Achyranthes aspera

Authors

  • Vinayak Upadhya Ethnomedicine Division, Regional Medical Research Centre (RMRC), Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum – 590 010, Karnataka, India
  • Gireesh M Ankad Ethnomedicine Division, Regional Medical Research Centre (RMRC), Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum – 590 010, Karnataka, India
  • Sandeep Ramchandra Pai Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre (RMRC), Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum – 590 010, Karnataka, India http://orcid.org/0000-0002-1133-8061
  • Harsha V Hegde Regional Medical Research Centre (RMRC), Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum – 590 010, Karnataka, India

DOI:

https://doi.org/10.14719/pst.2015.2.1.87

Keywords:

Achyranthes aspera, Achyranthes coynei, HPTLC

Abstract

Leaf and stem materials of Achyranthes coynei and Achyranthes aspera were used for HPTLC analysis. HPTLC plates were developed on n-hexane: ethyl acetate (5:1 v/v) solvent system. The densitometric profiles were evaluated to elucidate differences within and among the species. The Rf values and number of peaks obtained in densitrogram indicated chemical variation in the species. Although, both species had more or less equal number of peaks, their Rf values, %height and %area varied. Thus HPTLC analysis in absence of external standards, proved to be an informative tool for evaluating differences between these species and their parts.

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Author Biographies

Vinayak Upadhya, Ethnomedicine Division, Regional Medical Research Centre (RMRC), Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum – 590 010, Karnataka, India

Senior Research Fellow at Ethnomedicine Division, Regional Medical Research Centre, Belgaum

Gireesh M Ankad, Ethnomedicine Division, Regional Medical Research Centre (RMRC), Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum – 590 010, Karnataka, India

Scientist at Herbal medicine/Ethnomedicine Division, Regional Medical Research Centre, Belgaum

Sandeep Ramchandra Pai, Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre (RMRC), Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum – 590 010, Karnataka, India

Scientist at Plant Biotechnology and tissue culture division, Regional Medical Research Centre, Belgaum

Harsha V Hegde, Regional Medical Research Centre (RMRC), Indian Council of Medical Research (ICMR), Nehru Nagar, Belgaum – 590 010, Karnataka, India

Scientist C at Ethnomedicine Division, Plant Biotechnology and tissue culture division, Regional Medical Research Centre, Belgaum

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Published

01-01-2015

How to Cite

1.
Upadhya V, Ankad GM, Pai SR, Hegde HV. Comparative HPTLC analysis of stem and leaf of Achyranthes coynei with Achyranthes aspera. Plant Sci. Today [Internet]. 2015 Jan. 1 [cited 2024 Dec. 22];2(1):7-10. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/87

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Research communications

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