Anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle aqueous extract
DOI:
https://doi.org/10.14719/pst.2021.8.1.917Keywords:
LPRE, Protease, Anticoagulant, Anti-platelet aggregation, FibrinogenolyticAbstract
The current study explores the anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle extract (LPRE). It is firstly reported that LPRE has protein at a concentration of 1.5 mg/ml and further evaluated for protein in gel electrophoresis. The proteolytic activity of LPRE was evaluated using casein and gelatin as substrate. LPRE showed a specific activity of 0.35 U. LPRE increased the plasma clotting time significantly showing its anticoagulant property. LPRE hydrolyzed the A?, B? and ? chains of fibrinogen. Furthermore, LPRE significantly inhibited the platelet aggregation induced by agonist adenosine diphosphate (ADP).
Downloads
References
Roth GA, Johnson C, Abajobir A, Abd-Allah F, Abera SF, Abyu G. Global regional and national burden of cardiovascular diseases for 10 causes, 1990 to 2015. J Am College Cardiol. 2017; 70(1): 1-25. https://doi.org/10.1016/j.jacc.2017.04.052
Gaziano TA, Bitton A, Anand S, Abrahams-Gessel S, Murphy A. Growing epidemic of coronary heart disease in low- and middle-income countries. Curr Probl Cardiol. 2010; 35:72-115. https://dx.doi.org/10.1016%2Fj.cpcardiol.2009.10.002
Previtali E, Bucciarelli P, Passamonti SM, Martinelli I. Risk factors for venous and arterial thrombosis. Blood Transfus. 2011; 9(2):120-38. https://dx.doi.org/10.2450%2F2010.0066-10
Ovanesov MV. Study of the regulation of blood coagulation by factors VIIa and IXa. U.S Food & Drug Aministration, Biologic research project, New Hampshire Avenue, Silver Spring, MD. 2015.
Karch AM. Pharmacologial review: Drugs that alter blood coagulation. Am Nurse Today. 2012;7(11):26–31.
Aguirre J, Borgeat A. Drugs for Thromboprophylaxis: Unfractionated heparin, low molecular weight heparin, warfarin and fondaparinux. In: LLAU JV. Editor. Thromboembolism in Orthopedic Surgery, London: Springer, London, England. 2013;53-65.
Kumar S, Joseph L, George M, Sharma A. A review on anticoagulant / antithrombotic activity of natural plants used in traditional medicine. Int J Pharm Sci Rev Res. 2011;8:70-74.
Newman DJ, Cragg GM. Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod. 2012; 75:311-35. https://doi.org/10.1021/np200906s
Akram M, Rashid A. Anti-coagulant activity of plants: mini review. J Thromb Thrombolysis. 2017;44:406–11. https://doi.org/10.1007/s11239-017-1546-5
Lee W, Yang EJ, Ku SK, Song KS, Bae JS. Anticoagulant activities of oleanolic acid via inhibition of tissue factor expressions. The Korean Society for Biochemistry and Molecular Biology. 2012:45(7):390-95. https://doi.org/10.5483/bmbrep.2012.45.7.065
Manicam C, Abdullah JO, Tohit ERM, Seman Z, Chin SC, Hamid M. In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract: A preliminary novel finding. J Med Pla Res. 2010; 4(14): 1464-72. https://doi.org/10.5897/JMPR10.136
Sheila MK, Anselimo OM, Glaston MK. Physical characteristics, proximate composition and anti-nutritional factors in grains of lablab bean (Lablab purpureus (L.) Sweet) genotypes from Kenya. J App Bio. 2017; 114:11289-98. https://dx.doi.org/10.4314/jab.v114i1.2
Dilara P, Mir Muhammad NU, Siddiqu MI, Salma P, Mohammad S. Phytochemical screenings, thrombolytic activity and antimicrobial properties of the leaf extracts of Lablab purpureus. Am J Res Com. 2013;1(2):49-55.
Bhogireddy N, Vamsi Krishna AN, Ramesh B, Pradeep K, Reddy OVS, Gaddaguti V, Raj Kumar K, Pola PK, Venkataraman B. Anti-inflammatory and anti-diabetic activities with their other ethnomedicinal properties of the plants. J Med Pla Stu. 2013;1(5):87-96.
Kante K, Reddy CS. Anti diabetic activity of Dolichos lablab (seeds) in streptozotocin-nicotinamide induced diabetic rats. Hyg J Dru Med. 2013;(1):32-40.
Satake K, Okuyama T, Ohashi M, Shinoda T. The spectrophotometric determination of amine, amino acid and peptide with 2,4,6-trinitrobenzene1-sulfonic acid. J Biochem. 1960; 47:654–60. https://doi.org/10.1093/oxfordjournals.jbchem.a127107
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–85. https://doi.org/10.1038/227680a0
Nagaraju S, Kempaiah K. ‘Partitagin’, a unique ?, ? -fibrinogenase that inhibits platelet aggregation from Hippasa partita spider venom. Blood Coagul Fibrino. 2011;22:24–28. https://doi.org/10.1097/mbc.0b013e32833fcc15
Condrea E, Yang CC, Rosenberg P. Anticoagulant activity and plasma phosphatidylserine hydrolysis by snake venom phospholipases A2. Thromb Haemost. 1983;49:151.
Quick AJ. Stanley-Brown M, Bancroft FW. A study of the coagulation defect in hemophilia and in jaundice. Am J Med Sci. 1935;190:501–11. http://doi.org/10.1055/s-0038-1650068
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin-phenol reagent. J Biol Chem. 1951;193:265–75.
Rawski RI, Sanecki PT, D?ugan M, Kijowska K. The evidence of proteases in sprouted seeds and their application for animal protein digestion. Chem Zvesti. 2018; 72 (5): 1213-21. http://dor.org/10.1007/s11696-017-0341-2
Vinod G, Kusuma CG, Bhavana S, Sumachirayu CK, Nagaraju S. Anti-hemostatic protease from Jatropha curcas latex with fibrinogen lytic activity. J Pharmacogn Phytochem. 2019b; 8(1):1303-10.
Vinod G, Kusuma CG, Bhavana S, Sumachirayu CK, Ravikumar H, Nagaraju S. Potent procoagulant and platelet aggregation inducing serine protease from Tridax procumbens extract. Pharmacognosy Res. 2019c;11(4):363-70. http://doi.org/10.4103/pr.pr-4-19
Vinod G, Nagaraju S. Ethnomedicinal, phytochemical constituents and pharmacological activities of Tridax procumbens: a review. Int J Phar Pharm Sci. 2016;8(2):1-7.
Drake TA, Morrissey JH, Edgington TS. Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis. Ame J Pathol. 1989;134:1087-97.
Rachidi S, Aldin ES, Greenberg C, Sachs B, Streiff M, Zeidan AM. The use of novel oral anticoagulants for thromboprophylaxis after elective major orthopedic surgery. Exp Rev Hematol. 2013;6:677-95. https://dx.doi.org/10.1586%2F17474086.2013.853430
Zare H, Moosavi, Movahedi AA, Salami M, Mirzaei M, Saboury AA, Sheibani N. Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex. Phytochemistry. 2013;87:16-22. https://dx.doi.org/10.1016%2Fj.phytochem.2012.12.006
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 R S Thoyajakshi, D Poornima
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).