Anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle aqueous extract

Authors

DOI:

https://doi.org/10.14719/pst.2021.8.1.917

Keywords:

LPRE, Protease, Anticoagulant, Anti-platelet aggregation, Fibrinogenolytic

Abstract

The current study explores the anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle extract (LPRE). It is firstly reported that LPRE has protein at a concentration of 1.5 mg/ml and further evaluated for protein in gel electrophoresis. The proteolytic activity of LPRE was evaluated using casein and gelatin as substrate. LPRE showed a specific activity of 0.35 U. LPRE increased the plasma clotting time significantly showing its anticoagulant property. LPRE hydrolyzed the A?, B? and ? chains of fibrinogen. Furthermore, LPRE significantly inhibited the platelet aggregation induced by agonist adenosine diphosphate (ADP).

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References

Roth GA, Johnson C, Abajobir A, Abd-Allah F, Abera SF, Abyu G. Global regional and national burden of cardiovascular diseases for 10 causes, 1990 to 2015. J Am College Cardiol. 2017; 70(1): 1-25. https://doi.org/10.1016/j.jacc.2017.04.052

Gaziano TA, Bitton A, Anand S, Abrahams-Gessel S, Murphy A. Growing epidemic of coronary heart disease in low- and middle-income countries. Curr Probl Cardiol. 2010; 35:72-115. https://dx.doi.org/10.1016%2Fj.cpcardiol.2009.10.002

Previtali E, Bucciarelli P, Passamonti SM, Martinelli I. Risk factors for venous and arterial thrombosis. Blood Transfus. 2011; 9(2):120-38. https://dx.doi.org/10.2450%2F2010.0066-10

Ovanesov MV. Study of the regulation of blood coagulation by factors VIIa and IXa. U.S Food & Drug Aministration, Biologic research project, New Hampshire Avenue, Silver Spring, MD. 2015.

Karch AM. Pharmacologial review: Drugs that alter blood coagulation. Am Nurse Today. 2012;7(11):26–31.

Aguirre J, Borgeat A. Drugs for Thromboprophylaxis: Unfractionated heparin, low molecular weight heparin, warfarin and fondaparinux. In: LLAU JV. Editor. Thromboembolism in Orthopedic Surgery, London: Springer, London, England. 2013;53-65.

Kumar S, Joseph L, George M, Sharma A. A review on anticoagulant / antithrombotic activity of natural plants used in traditional medicine. Int J Pharm Sci Rev Res. 2011;8:70-74.

Newman DJ, Cragg GM. Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod. 2012; 75:311-35. https://doi.org/10.1021/np200906s

Akram M, Rashid A. Anti-coagulant activity of plants: mini review. J Thromb Thrombolysis. 2017;44:406–11. https://doi.org/10.1007/s11239-017-1546-5

Lee W, Yang EJ, Ku SK, Song KS, Bae JS. Anticoagulant activities of oleanolic acid via inhibition of tissue factor expressions. The Korean Society for Biochemistry and Molecular Biology. 2012:45(7):390-95. https://doi.org/10.5483/bmbrep.2012.45.7.065

Manicam C, Abdullah JO, Tohit ERM, Seman Z, Chin SC, Hamid M. In vitro anticoagulant activities of Melastoma malabathricum Linn. aqueous leaf extract: A preliminary novel finding. J Med Pla Res. 2010; 4(14): 1464-72. https://doi.org/10.5897/JMPR10.136

Sheila MK, Anselimo OM, Glaston MK. Physical characteristics, proximate composition and anti-nutritional factors in grains of lablab bean (Lablab purpureus (L.) Sweet) genotypes from Kenya. J App Bio. 2017; 114:11289-98. https://dx.doi.org/10.4314/jab.v114i1.2

Dilara P, Mir Muhammad NU, Siddiqu MI, Salma P, Mohammad S. Phytochemical screenings, thrombolytic activity and antimicrobial properties of the leaf extracts of Lablab purpureus. Am J Res Com. 2013;1(2):49-55.

Bhogireddy N, Vamsi Krishna AN, Ramesh B, Pradeep K, Reddy OVS, Gaddaguti V, Raj Kumar K, Pola PK, Venkataraman B. Anti-inflammatory and anti-diabetic activities with their other ethnomedicinal properties of the plants. J Med Pla Stu. 2013;1(5):87-96.

Kante K, Reddy CS. Anti diabetic activity of Dolichos lablab (seeds) in streptozotocin-nicotinamide induced diabetic rats. Hyg J Dru Med. 2013;(1):32-40.

Satake K, Okuyama T, Ohashi M, Shinoda T. The spectrophotometric determination of amine, amino acid and peptide with 2,4,6-trinitrobenzene1-sulfonic acid. J Biochem. 1960; 47:654–60. https://doi.org/10.1093/oxfordjournals.jbchem.a127107

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–85. https://doi.org/10.1038/227680a0

Nagaraju S, Kempaiah K. ‘Partitagin’, a unique ?, ? -fibrinogenase that inhibits platelet aggregation from Hippasa partita spider venom. Blood Coagul Fibrino. 2011;22:24–28. https://doi.org/10.1097/mbc.0b013e32833fcc15

Condrea E, Yang CC, Rosenberg P. Anticoagulant activity and plasma phosphatidylserine hydrolysis by snake venom phospholipases A2. Thromb Haemost. 1983;49:151.

Quick AJ. Stanley-Brown M, Bancroft FW. A study of the coagulation defect in hemophilia and in jaundice. Am J Med Sci. 1935;190:501–11. http://doi.org/10.1055/s-0038-1650068

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin-phenol reagent. J Biol Chem. 1951;193:265–75.

Rawski RI, Sanecki PT, D?ugan M, Kijowska K. The evidence of proteases in sprouted seeds and their application for animal protein digestion. Chem Zvesti. 2018; 72 (5): 1213-21. http://dor.org/10.1007/s11696-017-0341-2

Vinod G, Kusuma CG, Bhavana S, Sumachirayu CK, Nagaraju S. Anti-hemostatic protease from Jatropha curcas latex with fibrinogen lytic activity. J Pharmacogn Phytochem. 2019b; 8(1):1303-10.

Vinod G, Kusuma CG, Bhavana S, Sumachirayu CK, Ravikumar H, Nagaraju S. Potent procoagulant and platelet aggregation inducing serine protease from Tridax procumbens extract. Pharmacognosy Res. 2019c;11(4):363-70. http://doi.org/10.4103/pr.pr-4-19

Vinod G, Nagaraju S. Ethnomedicinal, phytochemical constituents and pharmacological activities of Tridax procumbens: a review. Int J Phar Pharm Sci. 2016;8(2):1-7.

Drake TA, Morrissey JH, Edgington TS. Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis. Ame J Pathol. 1989;134:1087-97.

Rachidi S, Aldin ES, Greenberg C, Sachs B, Streiff M, Zeidan AM. The use of novel oral anticoagulants for thromboprophylaxis after elective major orthopedic surgery. Exp Rev Hematol. 2013;6:677-95. https://dx.doi.org/10.1586%2F17474086.2013.853430

Zare H, Moosavi, Movahedi AA, Salami M, Mirzaei M, Saboury AA, Sheibani N. Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex. Phytochemistry. 2013;87:16-22. https://dx.doi.org/10.1016%2Fj.phytochem.2012.12.006

Published

01-01-2021

How to Cite

1.
Thoyajakshi RS, Poornima D. Anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle aqueous extract. Plant Sci. Today [Internet]. 2021 Jan. 1 [cited 2024 Nov. 4];8(1):89-94. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/917

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Section

Research communications