RAPD-based DNA fingerprinting in Lantana camara L. ecotypes and development of a digital database platform ‘LANRAD’
DOI:
https://doi.org/10.14719/pst.2016.3.2.177Keywords:
allelopathy, biocomputation tools, invasive potential, molecular diversity quadrat, relative competition intensityAbstract
This article has a correction. Please see:RETRACTION - 24 June 2016
With objectives to assess molecular diversity and digitization of molecular data in relation to invasive potential of Lantana camara L., leaves of 12 ecotypes collected from five different eco-climatic zones of Eastern Indian state of West Bengal, were subjected to RAPD fingerprinting. Invasive potential (IP) was assessed by allelopathic effects of Lantana leaf extracts on seed germination of target crop (lentil), relative competition intensity (RCI) between weed and target crop and abundance (AB) of Lantana in quadrats. Genetic diversity was found between zones and between ecotypes within a zone. Polymorphism varied 25-70 % with highest magnitude was found in Indo-Gangetic Basin, followed by Western dry land, and parts of temperate and sub-Himalayan zones of Indian Himalayas. Moderate polymorphism with 50 % polymorphic markers was observed in coastal ecotypes. Strong positive correlation existed between polymorphism %, IP, RCI and AB, and 12 ecotypes were clearly distinguished in four categories of invasiveness. IP1 represented worst invasiveness in Gangetic basin and Western dry land while the lowest (IP4) was observed in parts of temperate and sub-Himalayan region. The ‘Darjeeling’ ecotype in temperate Himalayas (2000 m, msl) was identified as moderately-high invasive (IP2 category), possessing two unique RAPD markers. Phylogenetic tree revealed nine groups of 12 ecotypes exhibiting close similarity within coastal zones and within Western dry land ecotypes. The data are now being digitized using bio computation tools/software in a bio-digital platform ‘LANRAD’ (Lantana Random Amplified DNA), as the first eco-genomic on-line platform of an alien invasive plant in India.
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