Amelioration of salinity stress by NaCl pretreatment with reference to sugar metabolism in legumes Cajanas cajan L. and Vigna mungo L.

  • Paramita Chatterjee Department of Botany, University of Calcutta
  • Sabarni Biswas Department of Botany, University of Calcutta
  • Asok Kumar Biswas University of Calcutta


The effect of salinity stress and its amelioration by pretreatment with low concentration of NaCl (50mM) on growth and sugar metabolism in arhar (cv. T120) and maskalai (cv. WBU109) seedlings were studied. Salinity was found to be more toxic for root growth than shoot growth. Fresh weight and dry weight of the seedlings gradually decreased with increasing concentrations of NaCl treatment. It was demonstrated that direct germination on NaCl solution increased both reducing sugar and non-reducing sugar contents while decreased the starch contents which were to some extent decreased by pretreatment of seeds with 50mM NaCl prior to germination in salt solutions. Salinity stress also affected the activites of different sugar metabolizing enzymes. The increase in the activities of starch phosphorylase, sucrose phosphate synthase, sucrose synthase and decrease in the activities of acid invertase were observed in directly salt treated test seedlings that were altered by pretreatment with sublethal concentration of NaCl in both the cultivars arhar (cv. T120) and maskalai (cv. WBU109) seeds. Thus the application of pretreatment by sublethal concentration of NaCl in both arhar (cv. T120) and maskalai (cv. WBU109) seeds exhibited significant alteration of all the partinent parameters tested under salinity stress and the effect of pretreatment in most of the parameters were more prominent in arhar (cv. T120) with compared to maskalai (cv. WBU109) seedlings.


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Author Biographies

Paramita Chatterjee, Department of Botany, University of Calcutta
Research scholar
Sabarni Biswas, Department of Botany, University of Calcutta
Research scholar
Asok Kumar Biswas, University of Calcutta

Department of Botany, University of Calcutta.

Professor in Botany


Borkowska, B. and J. Szczerba. 1991. Influence of Different Carbon Sources on Invertase Activity and Growth of Sour Cherry (Prunus cerasus L.) shoot cultures. J. Exp. Bot. 42: 911-915. doi:10.1093/jxb/42.7.911

Chaillou, P. B. and G. Guerrier. 1992. Salt responses in Lycopersicon esculentum calli and whole plants. J. Plant Physiol. 140: 494-501.

Das, P., P. Seal and A. K. Biswas. 2016. Regulation of Growth, Antioxidants and Sugar Metabolism in Rice (Oryza sativa L.) Seedlings by NaCl and Its Reversal by Silicon. Am. J. Plant Sci. 7: 623-638. doi:10.4236/ajps.2016.73055

Devi, S., R. Angrish, K. S. Datta and B. Kumar. 2008. Antioxidant defence system in wheat seedlings under sodium chloride stress: An inductive role of hydrogen peroxide. Indian J. Plant Physiol. 13: 22-26.

Dubey, R. S. and A. K. Singh. 1999. Salinity Induces Accumulation of Soluble Sugars and Alters the Activity of Sugar Metabolising Enzymes in Rice Plants. Biol. Plant. 42: 233-239. doi:10.1023/A:1002160618700

Evers, D., C. Schmit, Y. Mailliet and J. F. Hausman. 1997. Growth characteristics and biochemical changes of poplar shoots in vitro under sodium chloride stress. J. Plant Physiol. 151: 748-753.

Fiske, C. H. and Y. Subbarow. 1925. The colorimetric determination of phosphorus. J. Biol. Chem. 66: 375-400.

Flowers, T., P. F. Torke and A. R. Yeo. 1977. The mechanism of salt tolerance in halophytes. Annu. Rev. Plant Physiol. 28: 89-121.

Foyer, C. H., M. H. Valadier, A. Migge and T. W. Becker. 1998. Drought-induced effects on nitrate reductase activity and mRNA and on the coordination of nitrogen and carbon metabolism in maize leaves. Plant Physiol. 117: 283- 292.

Furuichi, T., I. C. Mori, K. Takahashi and S. Muto. 2001. Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants. Plant Cell Physiol. 42: 1149-1155. doi:10.1093/pcp/pce150

Gao, Z., M. Sagi, and S. H. Lips. 1998. Carbohydrate Metabolism in Leaves and Assimilate Partitioning in Fruits of Tomato (Lycopersicon esculentum L.) as Affected by Salinity. Plant Sci. 135: 149-159. doi:10.1016/s0168-9452(98)00085-5

Geigenberger, P. and M. Stitt. 1993. Sucrose synthase catalyses a readily reversible reaction in vivo in developing potato tubers and other plant tissues. Planta. 189: 329-339. doi:10.1007/BF00194429

Guy, C. L., J. L. Huber and S. C. Huber. 1992. Sucrose phosphate synthase and sucrose accumulation at low temperature, Plant Physiol. 100: 502-508.

Hawker, J. S. 1980. Invertases from Leaves of Phaseolus vulgaris Plant Grown on Nutrient Solutions Containing NaCl. Aust J Plant Physiol. 7: 67-72. doi:10.1071/PP9800067

Hebers, K. and V. Sonnewald. 1998. Altered gene expression brought about by inter- and intra-cellularly formed hexoses and its possible implications for plant-pathogen interactions. J. Plant Res. 111: 323-328.

Hubbard, N. L., S. C. Huber and D. M. Pharr. 1989. Sucrose Phosphate Synthase and Acid Invertase as Determinant of Sucrose Concentration in Developing Muskmelon (Cucumis melo L.) fruits. Plant Physiol. 9: 1527-1534. doi:10.1104/pp.91.4.1527

Huber, S. C. and T. Akazawa. 1986. A Novel Sucrose Synthase Pathway for Sucrose Degradation in Cultured Sycamore Cells. Plant Physiol. 81: 1008-1013. doi:10.1104/pp.81.4.1008

Krause, K. P., L. Hill, R. Reimholz, N. T. Hamborg, U. Sonnewald and M. Stitt. 1998. Sucrose metabolism in cold-stored potato tubers with decreased expression of sucrose phosphate synthase. Plant Cell Environ. 21: 285–299. doi:10.1046/j.1365-3040.1998.00271.x

Krishnamurthy, R., M. Anbazhgan and K. A. Bhagwat. 1987. Salt responses of enzymes from rice cultivars differing in salt tolerance. Curr. Sci. 56: 489-490.

Livingston, D. P. and C. A. Henson. 1998. Apoplastic Sugars, Fructans, Fructans Exohydrolase and Invertase in Winter Oat: Responses to Second-Phase Cold Hardening. Plant Physiol. 116: 403-408. doi:10.1104/pp.116.1.403

Lohaus, G., H. Winter, B. Riens and H. W. Heldt. 1995. Further Studies of the Phloem Loading Process in Leaves of Barley and Spinach. The Comparison of Metabolite Concentrations in the Apoplastic Compartment with those in the Cytosolic Compartment and in the Sieve Tubes. Bot. Acta. 108: 270-275. doi:10.1111/j.1438-8677.1995.tb00860.x

McCready, R. M., J. Guggolz, V. Silviera and H. S. Owens. 1950. Determination of Starch and Amylose in Vegetables. Anal. Chem. 22: 1156-1158. doi:10.1021/ac60045a016

Miller, G. L. 1972. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugars. Anal Chem. 31: 426- 428. doi:10.1021/ac60147a030

Miron, D. and A. A. Schaffer. 1991. Sucrose phosphate synthase, sucrose synthase and invertase activities in developing fruit of Lycopersicon esculentum Mill. and the sucrose accumulating Lycopersicon hirsutum Humb. and Bonpl. Plant Physiol. 95: 623-627. doi:10.1104/pp.95.2.623

Misra, N. and U. N. Dwivedi, 2004. Genotypic difference in salinity tolerance of green gram cultivars. Plant Sci. 166: 1135-1142. doi: 10.1016/j.plantsci.2003.11.028.

Nelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153: 375-380.

Parida, A. K., A. B. Das and P. Mohanty. 2004. Investigations on the Antioxidative Defence Responses to NaCl Stress in a Mangrove, Bruguiera parviflora: Differential Regulations of Isoforms of Some Antioxidative Enzymes. Plant Growth Regul. 42: 213-226. doi:10.1023/B:GROW.0000026508.63288.39

Pattanagul, W. and M. Thitisaksakul. 2008. Effects of salinity stress on growth and carbohydrate metabolism in three rice (Oryza sativa L.) cultivars differing in salinity tolerance. Indian J. Exp. Biol. 46: 736-742.

Pfeiffer, I. and U. Kutschera. 1996. Sucrose metabolism and lipid mobilization during light induced expansion of sunflower leaves. J. Plant Physiol. 147: 553-558.

Quick, W. P., G. Siegl, H. E. Neuhaus, R. Feil and M. Stitt. 1989. Short-Term Water Stress Leads to a Stimulation of Sucrose Synthesis by Activating Sucrose Phosphate Synthase. Planta. 177: 535-546. doi:10.1007/BF00392622

Ranwala, A. P. and W. B. Miller. 1998. Sucrose cleaving enzymes and carbohydrate pools in Lilium longiflorum floral organ. Physiol. Plant. 103: 541-550. doi:10.1034/j.1399-3054.1998.1030413.x

Richharia, A. P., K. Shah and R. S. Dubey. 1997. Nitrate reductase from rice seedlings: Partial purification, characterization and the effects of in situ and in vitro NaCl salinity. J. Plant Physiol. 151: 316-322.

Saha, P., P. Chatterjee and A. K. Biswas. 2010. NaCl pretreatment alleviates salt stress by enhancement of antioxidant defense and osmolyte accumulation in mungbean (Vigna radiata L. Wilczek). Indian J. Exp. Biol. 48: 593–600.

Somogyi, M. 1952. Notes on sugar determination. J. Biol. Chem. 195: 19-23.

Van den Ende, W., A. Michiels, K. Le Roy and A. Van Laere. 2002. Cloning of a Vacuolar Invertase from Belgian endive leaves (Cichorium intybus). Physiol. Plantarum, 115: 504-512. doi: 10.1034/j.1399-3054.2002.1150404.x

Widodo, P. J. H., E. Newbigin, M. Tester, A. Bacic and U. Roessner. 2009. Metabolic responses to salt stress in barley (Hordeum vulgare L.) cultivars, Sahara and Clipper, which differ in salinity tolerance. J. Exp. Bot. 60: 4089-4103. doi:10.1093/jxb/erp243.

Zhou, R., R. C. Silcher and B. Quebedeaux. 2002. Apple Leaf Sucrose Phosphate Synthase is inhibited by Sorbitol-6- Phosphate. Funct. Plant Biol. 29: 569-574. doi:10.1071/PP01123
How to Cite
CHATTERJEE, Paramita; BISWAS, Sabarni; BISWAS, Asok Kumar. Amelioration of salinity stress by NaCl pretreatment with reference to sugar metabolism in legumes Cajanas cajan L. and Vigna mungo L.. Plant Science Today, [S.l.], v. 4, n. 1, p. 28-40, jan. 2017. ISSN 2348-1900. Available at: <>. Date accessed: 17 dec. 2017. doi:
Research Articles


Arhar; Maskalai; NaCl; Sugar metabolism; Biochemical changes