Phytophthora infestans induced defense response in calli of wild and cultivated potato genotypes: Pathogen induced cell death in cultures - a marker for resistance

  • Kumari N Aruna Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bangalore 560065
  • Veena S Anil University of Agricultural Sciences, GKVK, Bangalore
  • B. T. Krishnaprasad Department of Agricultural Biotechnology, College of Agriculture, Hassan 573225


Late Blight caused by Phytophthora infestans (Mont.) de Bary is the most destructive foliar disease causing 30% yield losses in the potato (Solanum tuberosum L.) crop globally. Wild potato genotypes AC1 and AC4, and potato cultivar Kufri Girdhari are highly resistant, whereas wild genotype AC6, and cultivars Kufri Chandramuki and Kufri Jyoti are susceptible to Late Blight. In the current study, the calli of these six potato genotypes were used to understand the mechanism of cellular resistance to Late Blight. Exposure to P. infestans or its elicitors significantly induced peroxidase (POX) and superoxide dismutase (SOD) activities, and induced accumulation of phenolics and flavonoids, indicating the capability of the calli cells to mount a defense response. The study is the first to report the extracellular secretion of defense enzymes, SOD and POX when cells encounter the pathogen, implicating a similar whole-plant phenomenon of enhanced defense in the apoplast. Interestingly, the calli of resistant genotypes showed poor survival upon exposure to pathogen or when grown on elicitor medium, while the susceptible genotypes showed better survival. The percentage of calli cells accumulating intracellular H2O2 was high in resistant genotypes, and directly correlated with the observed higher cell death. The study shows that H2O2 accumulation in the cells of resistant genotypes is indeed self-destructive, a whole plant phenomenon termed hypersensitive response - cell death at site of infection. The potato callus system thus can be used to gain new insights into the plant-defense response to P. infestans.


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Author Biography

Veena S Anil, University of Agricultural Sciences, GKVK, Bangalore

Plant biochemist at the University of Agricultural Sciences, Bangalore, currently working on Late blight resistance mechanisms in potato genotypes both at the whole plant and cellular levels. Earlier worked as Visiting Fellow at NCBS Bangalore on salt tolerance mecahanisms in rice. PhD degree from the department of Biochemistry, Indian Institute of Science, Bangalore.


Ainsworth, E. A., and K. M. Gillespie. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols 2: 875 – 877.

Alscher, R. G., N. Erturk, and L. S. Heath. 2002. Role of superoxide dismutase (SODs) in controlling oxidative stress in plants. Journal of Exp. Bot 53: 1331-1341.

Anil, V, S., A. C. Harmon, and S. K. Rao. 2000. Spatio-temporal accumulation and activity of calcium-dependent protein kinases during embryogenesis, seed development, and germination in sandalwood. Plant Physiol. 122: 1035–1043.

Anil, V. S., H. Krishnamurthy, and M. K. Mathew. 2007. Limiting Cytosolic Na+ Confers Salt Tolerance to Rice Cells in Culture: A Two-Photon Microscopy Study of SBFI Loaded Cells. Physiologia Plantarum 129: 607–621.

Anil, V. S., Venkatesh, and P. Gowda. 2013. Defense responses in Potato genotypes against Late Blight pathogen Phytopthora Infestans. National Conference on Crop improvement and adaptive strategies to meet challenges of climate change, GKVK, Bangalore, Feb 22-24.

Asada, K., and M. Takahashi. 1987. Production and scavenging of active oxygen in photosynthesis. In : D. J. Kyle, C. B. Osmond, C. J. Amtzen, Eds, Photoinhibition (Topics in Photosynthesis), Vol 9. Elsevier, Amsterdam, pp 227–287.

Beckman, C. H. 2000. Phenolic-storing cells: keys to programmed cell death and periderm formation in wilt disease resistance and in general defense responses in plants. Physiol. Mol. Plant Pathol., 57:101-110.

Castillo, F. I., I. Penel, and H. Greppin. 1984. Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol., 74: 846-851.

Caten, C. E., and J. L. Jinks 1968. Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Can. J. Bot., 46: 329-332.

Chaman, M. E., L. J. Corcuera, G, E. Zuniga, L. Cardemil, and V. H. Argandona. 2001, Induction of soluble and cell wall peroxidases by aphid infestation in barley. L. Agric Food Chem. 49: 2249-2253.

Chkhubianishvil, I. E., N. Kacharava, Z. Badridze, S. Chanishvili, and T. Kurdadze, 2011. Activity of Peroxidase, Catalase and Content of Total Proteins in Leaves of some Herbaceous Plants of High Mountains of the Caucasus. Bull. Georg. Natl. Acad. Sci. 5: 96-101.

Christensen, J. H., S. Overney, S. Rohde, W. A. Diaz, G. Bauw, P. Simon, M. Van montagu., and W. boerjan. 2001. The syringaldazine-oxidizing peroxidase PXP 3-4 from poplar xylem: cDNA isolation, characterization and expression. Plant Mol. Biol., 47: 581-593.

Dat, J., S. Vandenabeele, E. Vranova, M. Van Montagu, D. Inze, and F. Van Breusegem. 2000. Dual action of the active oxygen species during plant stress responses. Cell. Mol. Life Sci., 57: 779-795.

Dhindsa, R. H., R. Plumb-dhindsa, and T. A. Thorpe. 1981. Leaf senescence correlated with increased level of membrane permeability, lipid peroxidation and decreased level of SOD and CAT. J. Exptal. Bot., 32: 93-101.

Dixon, R. A., and C. J. Lamb. 1990. Molecular communication in interactions between plant and microbial pathogens. Ann. Rev. Plant Physiol. Plant Mol. Biol., 41: 339-367.

Ebel, J., and H. Grisebach. 1988. Defense strategies of soybean against the fungus Phytophthora megasperma: a molecular analysis. Trends in Biochemical Sci., 13: 23-27.

Gechev, T. S., F. V. Breusegem, J. M. Stone, I. Denev, and C. Loloi. 2006. Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bioessays 28: 1091–1101.

Grant, J. J., and G. J. Loake. 2000. Ole of reactive oxygen intermediates and cognate redox signaling in disease resistance. Plant Physiol. 124: 21–29.

Heath, M. C. 2000. Hypersensitive response-related death. Plant Mol. Biol., 44: 321–334.

Heng-Moss, T. M., G. Sarath, F. Baxendale, D. Novak, S. Bose, N. Xinhi, and S. Quisenberry. 2004. Characterization oxidative enzyme changes in buffalo grasses challenged by Blissus occiduus. J. Econ. Entomol. 97: 1086-1095.

Hernandez, J. A., A. Jimenez., P. Mullineaux, and Sevilla, F. 2000. Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defenses. Plant Cell Environ. 23: 853–862.

Hijmans, R. J., T. Gavrilenko, S. Stephenson, J. Bamberg, A. Salas, and D. M. Spooner. 2007. Geographical and environmental range expansion through polyploidy in wild potatoes (Solanum section Petota). Glob. Ecol. Biogeogr. 16: 485–495.

Kavitha, R., and S. Umesha. 2008. Regulation of defense-related enzymes associated with bacterial spot resistance in tomato. Phytoparasitica 36: 144.

Lamb, C., and R. A. Dixon. 1997. The oxidative burst in plant disease resistance. Annu Rev Plant Physiol. Plant Mol. Biol. 48: 251–275.

Lowry, D. H., N. J. Rosebrough, A. L. Farr, and J. L. Randall. 1951. Protein measurement with Folin-phenol reagent. Biol. Chem. 193: 265-275.

Mittler, R., and E. Lani. 1996. Sacrifice in the face of foes: Pathogen-induced programmed cell death in plants. Trends Microbiol. 4: 10-15.

Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissues cultures. Physiol. Plant. 15: 473–497.

Noctor, G., I. A. M. Aris, L. Jouanin, K. J. Kunert, H. Rennenberg, and C. H. Foyer. 1998. Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants. J. Exp. Bot., 49: 623-647.

Orozco-Cardenas, M., and C. A. Ryan. 1999. Hydrogen peroxide is generated systemically in plant leaves by wounding and system in via the octadecanoid pathway. Proc. Natl. Acad. Sci. USA. 96: 6553-7.

Silva, M. C., M. Nicole., L. Guerra-Guimarães, and C. J. Rodrigues Jr. 2002. Hypersensitive cell death and post-haustorial defence responses arrest the orange rust (Hemileia vastatrix) growth in resistant coffee leaves. Physiol. Mol. Plant Pathol. 60: 169-183.

Silva, M. C., L. Guerra-Guimarães, A. Loureiro, M. R. Nicole. 2008. Involvement of peroxidases in the coffee resistance to orange rust (Hemileia vastatrix). Physiol. Mol. Plant Pathol. 72: 29-38.

Smith, M. K., and J. A. MCCOMB. 1981. Effect of NaCl on the growth of whole plants and their corresponding callus cultures. Aust. J. Plant Physiol. 8: 267–275.

Strogonov, B. P. 1973. Structure and Function of Plant Cells in Saline Habitat, p. 284, Wiley, New York and Toronto.

Treutter, D. 2005. Significance of flavonoids in plant resistance and enhancement of their biosynthesis. Plant Biol., 7: 581–591.

Zhang, Y., S. Xu, P. Ding, D. Wang, Y. T. Cheng, J. He, M. Gao, F. Xu, Y. Li, and Z. Zhu. 2009. Control of salicylic acid synthesis and systemic acquired resistance by two members of a plant-specific family of transcription factors. Proc. Natl. Acad. Sci. USA. 107: 18220–18225.
How to Cite
ARUNA, Kumari N; ANIL, Veena S; KRISHNAPRASAD, B. T.. Phytophthora infestans induced defense response in calli of wild and cultivated potato genotypes: Pathogen induced cell death in cultures - a marker for resistance. Plant Science Today, [S.l.], v. 4, n. 3, p. 105-120, july 2017. ISSN 2348-1900. Available at: <>. Date accessed: 19 jan. 2018. doi:
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