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Plant Science Today (PST)

Research Articles

Early Access

Genetic diversity analysis among lentil genotypes for stemphylium blight tolerance using SSR markers

DOI
https://doi.org/10.14719/pst.10367
Submitted
30 June 2025
Published
10-07-2026
Versions

Abstract

In Bangladesh, lentil is a significant drought-tolerant pulse crop that offers essential minerals and protein. However, stemphylium blight (SB), which is caused by Stemphylium botryosum, a dematiaceous hyphomycete fungi, consists of almost 150 species, affecting a large amount of crops, leguminous as well as non-leguminous crops and significantly influencing the reduction of crop productivity. 60-65 % yield losses are caused by this disease, especially when favourable conditions of high humidity and moderate temperatures are present. To determine genetic diversity among seven mutant lines (LM-1, LM-2, LM-3, LM-4, LM-20-4, LM-99-8 and LM-118-9), which were collected from BINA and two existing varieties, namely Binamasur-8 and BARI Masur-1, nine SSR (simple sequence repeat) markers were utilised. A CTAB-based DNA extraction method was followed by using a specific kit. The range of PIC (polymorphic information content) values of SSR primers extended from 0.09 to 0.33, with SSR156 marking the upper values and ALD42 marking the lower values, which suggests that there is moderately to low polymorphism among lentil mutants and existing varieties. The highest Jaccard’s similarity was found between LM-3 and LM-4, nearly 100 %; in contrast, the lowest was found between LM-118-9 and LM-1, which helps to identify closely related genotypes. The Euclidean distance study revealed that LM-118-9/LM-4 cross with BARI Masur-1 could be used in developing stemphylium blight-resistant lentil varieties in future. Breeding programmes focuses on stress tolerance will be carried out, ensuring a stable production rate.

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