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Plant Science Today (PST)

Research Articles

Early Access

Seed germination and morphological responses of cotton under model salt stress

DOI
https://doi.org/10.14719/pst.10481
Submitted
6 July 2025
Published
22-10-2025
Versions

Abstract

Soil salinization is a major environmental stress that restricts plant growth and reduces crop yields. Developing salt-tolerant crop varieties using classical, molecular genetics methods and understanding the mechanisms of salt resistance is essential for sustainable crop improvement. This study evaluated seed germination and vegetative morphology of upland cotton (Gossypium hirsutum L.) under model salt stress, comparing Uzbek classical breeding varieties of AN-Bayovut-2, Tashkent-6, Namangan-77 and the Coker-312 variety with biotechnological knock-out varieties of the Porloq series viz. Porloq-1, Porloq-2, Porloq-3 and Porloq-4. An analysis of the results of seed germination parameters: relative germination (RG), average germination time (AGT), germination index (GI) and germination energy (GE) showed that the most resistant to salinity (NaCl concentrations— 50, 100, 150 and 200 mM) was the biotechnological gene-knockout Porloq-4 variety and the least resistant was the Coker-312 variety of classical breeding. A study of morphological parameters showed that 100 mM NaCl causes a slowdown in the growth of roots and shoots, as well as biomass gain in both genotypes. However, in the Porloq-4 biotechnological variety, the negative effects of NaCl were less pronounced compared to the salt-sensitive Coker-312 variety.

References

  1. 1. Sahab S, Suhani I, Srivastava V, Chauhan PS, Singh RP, Prasad V. Potential risk assessment of soil salinity to agroecosystem sustainability: Current status and management strategies. Sci Total Environ. 2021;764:144164. https://doi.org/10.1016/j.scitotenv.2020.144164
  2. 2. Singh A. Soil salinity: a global threat to sustainable development. Soil Use Manag. 2022;38:39-67. https://doi.org/10.1111/sum.12772
  3. 3. Hopmans JW, Qureshi AS, Kisekka I, Munns R, Grattan SR, Rengasamy P, et al. Chapter one - Critical knowledge gaps and research priorities in global soil salinity. In: Sparks DL, editor. Adv Agron. 2021;169:1-191. https://doi.org/10.1016/bs.agron.2021.03.001
  4. 4. Stavi I, Thevs N, Priori S. Soil salinity and sodicity in drylands: A review of causes, effects, monitoring and restoration measures. Front Environ Sci. 2021;9:712831. https://doi.org/10.3389/fenvs.2021.712831
  5. 5. Balasubramaniam T, Shen G, Esmaeili N, Zhang H. Plants' response mechanisms to salinity stress. Plants (Basel). 2023;12(12):2253. https://doi.org/10.3390/plants12122253
  6. 6. Foronda DA. Reclamation of a saline-sodic soil with organic amendments and leaching. Environ Sci Proc. 2022;16(1):56. https://doi.org/10.3390/environsciproc2022016056
  7. 7. Fu H, Yang Y. How plants tolerate salt stress. Curr Issues Mol Biol. 2023;45(7):5914-34. https://doi.org/10.3390/cimb45070374
  8. 8. Maryum Z, Luqman T, Nadeem S, Khan SMUD, Wang B, Ditta A, et al. An overview of salinity stress, mechanism of salinity tolerance and strategies for its management in cotton. Front Plant Sci. 2022;13:907937. https://doi.org/10.3389/fpls.2022.907937
  9. 9. Ma L, Liu X, Lv W, Yang Y. Molecular mechanisms of plant responses to salt stress. Front Plant Sci. 2022;13:934877. https://doi.org/10.3389/fpls.2022.934877
  10. 10. Xiao F, Zhou H. Plant salt response: Perception, signaling and tolerance. Front Plant Sci. 2023;13:1053699. https://doi.org/10.3389/fpls.2022.1053699
  11. 11. Mamatkulova SK, Kamburova VS, Latypova EA, Mamatkulova GF, Boryaev GI. Comparative biochemical analysis of biotech cotton variety Porloq-4. IOP Conf Ser Earth Environ Sci. 2022;953:012040. https://doi.org/10.1088/1755-1315/953/1/012040
  12. 12. Kamburova VS, Ubaydullaeva KA, Shermatov SE, Buriev ZT, Charishnikova OS, Nebesnaya KS, et al. Influence of RNA interference of phytochrome A1 gene on activity of antioxidant system in cotton. Physiol Mol Plant Pathol. 2022;117:101751. https://doi.org/10.1016/j.pmpp.2021.101751
  13. 13. Abdurakhmonov IY, Buriev ZT, Saha S, Jenkins JN, Abdukarimov A, Pepper AE. Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L. Nat Commun. 2014;5:3062. https://doi.org/10.1038/ncomms4062
  14. 14. Munawar W, Hameed A, Khan MKR. Differential morphophysiological and biochemical responses of cotton genotypes under various salinity stress levels during early growth stage. Front Plant Sci. 2021;12:622309. https://doi.org/10.3389/fpls.2021.622309
  15. 15. Zhang L, Ma H, Chen T, Pen J, Yu S, Zhao X. Morphological and physiological responses of cotton (Gossypium hirsutum L.) plants to salinity. PLoS One. 2014;9(11):e112807. https://doi.org/10.1371/journal.pone.0112807
  16. 16. Zhang T, Fan S, Xiang Y, Zhang S, Wang J, Sun Q. Non-destructive analysis of germination percentage, germination energy and simple vigour index on wheat seeds during storage by Vis/NIR and SWIR hyperspectral imaging. Spectrochim Acta A Mol Biomol Spectrosc. 2020;239:118488. https://doi.org/10.1016/j.saa.2020.118488
  17. 17. Zahid Z, Khan MKR, Hameed A, Akhtar M, Ditta A, Hassan HM, et al. Dissection of drought tolerance in upland cotton through morpho-physiological and biochemical traits at seedling stage. Front Plant Sci. 2021;12:627107. https://doi.org/10.3389/fpls.2021.627107
  18. 18. Liu Y, Ye N, Liu R, Chen M, Zhang J. H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination. J Exp Bot. 2010;61(11):2979-90. https://doi.org/10.1093/jxb/erq125
  19. 19. Munns R. Comparative physiology of salt and water stress. Plant Cell Environ. 2002;25(2):239-50. https://doi.org/10.1046/j.0016-8025.2001.00808.x
  20. 20. Mwando E, Han Y, Angessa TT, Zhou G, Hill CB, Zhang XQ, et al. Genome-wide association study of salinity tolerance during germination in barley (Hordeum vulgare L.). Front Plant Sci. 2020;11:118. https://doi.org/10.3389/fpls.2020.00118
  21. 21. Uçarlı C. Effects of salinity on seed germination and early seedling stage. In: Fahad S, Saud S, Chen Y, Wu C, Wang D, editors. Abiotic stress in plants. London: IntechOpen. 2020. https://doi.org/10.5772/intechopen.93647
  22. 22. Llanes A, Andrade A, Masciarelli O, Alemano S, Luna V. Drought and salinity alter endogenous hormonal profiles at the seed germination phase. Seed Sci Res. 2016;26:1-13. https://doi.org/10.1017/S0960258515000331
  23. 23. Abdurakhmonov IY, Salakhutdinov I, Kamburova V. Cotton breeding in the view of abiotic and biotic stresses: challenges and perspectives. In: Abdurakhmonov IY, editor. Cotton. Rijeka: IntechOpen. 2022. https://doi.org/10.5772/intechopen.104761
  24. 24. Chaudhary MT, Majeed S, Rana IA, Ali Z, Jia Y, Du X, et al. Impact of salinity stress on cotton and opportunities for improvement through conventional and biotechnological approaches. BMC Plant Biol. 2024;24(1):20. https://doi.org/10.1186/s12870-023-04558-4
  25. 25. Gul N, Khan Z, Shani MY, Hafiza BS, Saeed A, Khan AI, et al. Identification of salt-resilient cotton genotypes using integrated morpho-physiological and biochemical markers at the seedling stage. Sci Rep. 2025;15(1):5276. https://doi.org/10.1038/s41598-025-89582-0

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