Research Articles
Vol. 13 No. 3 (2026)
Effects of green synthesised silver nanoparticles on growth, nutrient composition and antioxidant activity of hydroponically grown barley (Hordeum vulgare L.)
Department of Botany, Faculty of Science, Aswan University, Aswan 81528, Egypt
Department of Botany, Faculty of Science, Aswan University, Aswan 81528, Egypt
Department of Biology, College of Science, Jouf University, Sakaka 2014, Al-Jouf, Saudi Arabia
Department of Botany, Faculty of Science, Aswan University, Aswan 81528, Egypt
Department of Botany, Faculty of Science, Aswan University, Aswan 81528, Egypt
Abstract
Barley (Hordeum vulgare L.) was hydroponically grown under different concentrations of green biosynthesised silver nanoparticles (Ag NPs) [0 (control), 20, 40, 60, 80 and 100 ppm] using 3 replicates. Different growth attributes such as the rate of germination, biomass, plant height, root elongation and seedling vigour index (SVI) were evaluated. Nitrogen (N), phosphorous (P), potassium (K), calcium (Ca) and magnesium (Mg) were analysed. Proximate composition including carbohydrates, proteins and crude fibre was analysed. Bioactive metabolites such as ascorbic acid, carotenoids, flavonoids and phenolics were measured. Total antioxidant capacity (TAC) and radical scavenging activity (RSA) were assessed. Plants treated with 100 ppm Ag NPs demonstrated the highest growth attributes and protein content, showing increases of 27.25 % in plant height, 33.3 % in root length, 36.46 % in SVI and 68.71 % in protein content compared to the control group. The sprouts of plants treated with 60 ppm Ag NPs demonstrated the highest levels of P, K and flavonoids, with increases of 174.04 %, 81.84 % and 98.32 %, respectively. Green synthesised Ag NPs improved growth and nutritional quality of hydroponic barley, with optimal effects depending on concentration.
References
- 1. Bulcha B, Diba D, Yusuf H. Forage Yield and nutritive values of selected improved barley varieties under hydroponic system. J Sci Technol Arts Res. 2024;13(1):151–67.
- 2. Arif M, Iram A, Fayyaz M, Abd El-Hack ME, Taha AE, Al-Akeel KA, et al. Feeding barley and corn hydroponic based rations improved digestibility and performance in Beetal goats. J King Saud Univ. 2023;35(2):102457. https://doi.org/10.1016/j.jksus.2022.102457
- 3. Baye W, Moges F, Andualem D. Effect of hydroponic barley fodder on growth performance, carcass yield and carcass quality of cobb 500 broilers. Heliyon. 2024;10(13). https://doi.org/10.1016/j.heliyon.2024.e33909
- 4. Jakhar AM, Aziz I, Kaleri AR, Hasnain M, Haider G, Ma J, et al. Nano-fertilizers: A sustainable technology for improving crop nutrition and food security. NanoImpact. 2022;100411. https://doi.org/10.1016/j.impact.2022.100411
- 5. Ayenew BM, Satheesh N, Zegeye ZB, Kassie DA. A review on the production of nanofertilizers and its application in agriculture. Heliyon. 2025. https://doi.org/10.1016/j.heliyon.2024.e41243
- 6. Patel C, Singh J, Karunakaran A, Ramakrishna W. Evolution of nano-biofertilizer as a green technology for agriculture. Agriculture. 2023;13(10):1865. https://doi.org/10.3390/agriculture13101865
- 7. Khan S, Zahoor M, Khan RS, Ikram M, Islam NU. The impact of silver nanoparticles on the growth of plants: The agriculture applications. Heliyon. 2023;9(6). https://doi.org/10.1016/j.heliyon.2023.e16928
- 8. Jadoon L, Gul A, Fatima H, Babar MM. Nano-elicitation and hydroponics: a synergism to enhance plant productivity and secondary metabolism. Planta. 2024;259(4):80. https://doi.org/10.1007/s00425-024-04353-x
- 9. Metwaly FEM, Moghazy MA, Sheded MG, Mohamed AAA. Green synthesis of silver nanoparticles using leaf extract of the hydrophyte Persicaria senegalensis: Preparation and antioxidant activity. Inorg Nano-Metal Chem. 2024;1–11. https://doi.org/10.1080/24701556.2024.2354504
- 10. Emam MSA. The sprout production and water use efficiency of some barley cultivars under intensive hydroponic system. Middle East J Agric Res. 2016; 5(2):161–170
- 11. Jaiman RK, Acharya SK, Pathan NP, Deshmukh AJ, Desai HA, Patel PK, et al. In vitro effect of seed bio-priming techniques on seed germination and seedling vigour of few vegetable crops. J Appl Nat Sci. 2020;12(4). https://doi.org/10.31018/jans.v12i4.2422
- 12. Walinga I, Van Der Lee JJ, Houba VJG, Van Vark W, Novozamsky I. Plant analysis manual. Springer Science & Business Media; 2013.
- 13. Thomas RL, Sheard RW, Moyer JR. Comparison of conventional and automated procedures for nitrogen, phosphorus and potassium analysis of plant material using a single digestion 1. Agron J. 1967;59(3):240–3. https://doi.org/10.2134/agronj1967.00021962005900030010x
- 14. Mariotti F, Tomé D, Mirand PP. Converting nitrogen into protein—beyond 6.25 and Jones’ factors. Crit Rev Food Sci Nutr. 2008;48(2):177–84. https://doi.org/10.1080/10408390701279749
- 15. Brooks JR, Griffin VK, Kattan MW. A modified method for total carbohydrate analysis of glucose syrups, maltodextrins and other starch hydrolysis products. Cereal Chem. 1986;63(5):465–7.
- 16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193:265–275. https://doi.org/10.1016/S0021-9258(19)52451-6
- 17. Busuttil-Griffin F, Shoemake C, Attard E, Azzopardi LM. Crude fibre determination of Malva sylvestris L. and evaluation of its faecal bulking and laxative properties in rats. Int J Biol. 2015;7(4):1–8.
- 18. Evana E, Barek MS. Determination of vitamin C (Ascorbic Acid) contents in two varieties of melon fruits (Cucumis melo L.) by iodometric titration. Fuller J Chem. 2021;6(2):143–7. https://doi.org/10.5539/ijb.v7n4p1
- 19. Hong HT, Takagi T, O’Hare TJ. An optimal saponification and extraction method to determine carotenoids in avocado. Food Chem. 2022;387:132923. https://doi.org/10.1016/j.foodchem.2022.132923
- 20. Agbo MO, Uzor PF, Nneji UNA, Odurukwe CUE, Ogbatue UB, Mbaoji EC. Antioxidant, total phenolic and flavonoid content of selected Nigerian medicinal plants. Dhaka Univ J Pharm Sci. 2015;14(1):35–41. https://doi.org/10.3329/dujps.v14i1.23733
- 21. Singelton Jr RA, Straits BC, Straits MM. Approches to Social Researches. New York: Oxford Univerity Press; 1999. p. 572.
- 22. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337–41. https://doi.org/10.1006/abio.1999.4019
- 23. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem. 1992;40(6):945–8. https://doi.org/10.1021/jf00018a005
- 24. Zaim NSHBH, Tan HL, Rahman SMA, Abu Bakar NF, Osman MS, Thakur VK, et al. Recent advances in seed coating treatment using nanoparticles and nanofibers for enhanced seed germination and protection. J Plant Growth Regul. 2023;42(12):7374–402. https://doi.org/10.1007/s00344-023-11038-4
- 25. Acharya P, Jayaprakasha GK, Crosby KM, Jifon JL, Patil BS. Green-synthesized nanoparticles enhanced seedling growth, yield and quality of onion (Allium cepa L.). ACS Sustain Chem Eng. 2019;7(17):14580–90. https://doi.org/10.1021/acssuschemeng.9b02180
- 26. Shaikhaldein HO, Al-Qurainy F, Babiker KA, Nadeem M, Khan S, Tarroum M, et al. Evaluating impacts of biosynthetic silver nanoparticles on morphophysiological responses in barley (Hordeum vulgare L.). J Nanomater. 2024;2024(1):7524774. https://doi.org/10.1155/2024/7524774
- 27. Sabir S, Arshad M, Satti SH. Effect of green synthesized silver nanoparticles on seed germination and seedling growth in wheat. Int J Agron Agric Res. 2018;12:1–7.
- 28. Guzmán-Báez GA, Trejo-Téllez LI, Ramírez-Olvera SM, Salinas-Ruíz J, Bello-Bello JJ, Alcántar-González G, et al. Silver nanoparticles increase nitrogen, phosphorus and potassium concentrations in leaves and stimulate root length and number of roots in tomato seedlings in a hormetic manner. Dose-Response. 2021;19(4):15593258211044576. https://doi.org/10.1177/15593258211044576
- 29. Karim S, Kayani S, Akhtar W, Fatima I, Nazir M, Zaman W. Biogenic synthesis of silver nanoparticles using Funaria hygrometrica Hedw. and their effects on the growth of Zea mays seedlings. Microsc Res Tech. 2023;86(6):686–93. https://doi.org/10.1002/jemt.24309
- 30. Latif H, Ghareib M, Tahon M. Phytosynthesis of silver nanoparticles using leaf extracts from Ocimum basilicum and Mangifira indica and their effect on some biochemical attributes of Triticum aestivum. Gesunde Pflanz. 2017;69(1). https://doi.org/10.1007/s10343-017-0385-9
- 31. Nongbet A, Mishra AK, Mohanta YK, Mahanta S, Ray MK, Khan M, et al. Nanofertilizers: a smart and sustainable attribute to modern agriculture. Plants. 2022;11(19):2587. https://doi.org/10.3390/plants11192587
- 32. Miyahira RF, Lopes J de O, Antunes AEC. The use of sprouts to improve the nutritional value of food products: A brief review. Plant Foods Hum Nutr. 2021;76(2):143–52. https://doi.org/10.1007/s11130-021-00888-6
- 33. Jankovskis L, Kokina I, Plaksenkova I, Jermaļonoka M. Impact of different nanoparticles on common wheat (Triticum aestivum L.) plants, course and intensity of photosynthesis. Sci World J. 2022;2022. https://doi.org/10.1155/2022/3693869
- 34. Sadak MS. Impact of silver nanoparticles on plant growth, some biochemical aspects and yield of fenugreek plant (Trigonella foenum-graecum). Bull Natl Res Cent. 2019;43(1):1–6. https://doi.org/10.1186/s42269-019-0259-7
- 35. Farghaly FA, Nafady NA. Green synthesis of silver nanoparticles using leaf extract of Rosmarinus officinalis and its effect on tomato and wheat plants. J Agric Sci. 2015;7(11):277. https://doi.org/10.5539/jas.v7n11p277
- 36. Salama HMH. Effects of silver nanoparticles in some crop plants, common bean (Phaseolus vulgaris L.) and corn (Zea mays L.). Int Res J Biotechnol. 2012;3(10):190–7.
- 37. Islam MR, Sattar D e shahwar, Sattar H, Perveen S, Akhlaq L. The effect of different concentration of silver nanoparticles on the growth, bioactive compound and scanning electron microscopy of germinated kidney beans (Phaseolus vulgaris). Bionanoscience. 2023;1–10. https://doi.org/10.1007/s12668-023-01108-y
- 38. Wu P, Li B, Liu Y, Bian Z, Xiong J, Wang Y, et al. Multiple physiological and biochemical functions of ascorbic acid in plant growth, development and abiotic stress response. Int J Mol Sci. 2024;25(3):1832. https://doi.org/10.3390/ijms25031832
- 39. El-Beltagi HS, Ahmad I, Basit A, Shehata WF, Hassan U, Shah ST, et al. Ascorbic acid enhances growth and yield of sweet peppers (Capsicum annum) by mitigating salinity stress. Gesunde Pflanz. 2022;74(2):423–33. https://doi.org/10.1007/s10343-021-00619-6
- 40. Zhu Y, Wang L, Ma J, Li Y, Chen F, Peijnenburg W. Comparative physiological and metabolomics analyses using Ag⎯ NPs and HAS31 (PGPR) to alleviate Cr stress in barley (Hordeum vulgare L.). Environ Pollut. 2023;122010. https://doi.org/10.1016/j.envpol.2023.122010
- 41. Khan M, Khan AU, Moon IS, Felimban R, Alserihi R, Alsanie WF, et al. Synthesis of biogenic silver nanoparticles from the seed coat waste of pistachio (Pistacia vera) and their effect on the growth of eggplant. Nanotechnol Rev. 2021;10(1):1789–800. https://doi.org/10.1515/ntrev-2021-0107
- 42. Mubeen B, Hasnain A, Jie W, Zheng H, Peijnenburg WJGM, Rozali SE, et al. enhanced production of active photosynthetic and biochemical molecules in Silybum marianum L. using biotic and abiotic elicitors in hydroponic culture. Molecules. 2023;28(4):1716. https://doi.org/10.3390/molecules28041716
- 43. Krishnaraj C, Jagan EG, Ramachandran R, Abirami SM, Mohan N, Kalaichelvan PT. Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. plant growth metabolism. Process Biochem. 2012;47(4):651–8. https://doi.org/10.1016/j.procbio.2012.01.006
- 44. Chung IM, Rajakumar G, Thiruvengadam M. Effect of silver nanoparticles on phenolic compounds production and biological activities in hairy root cultures of Cucumis anguria. Acta Biol Hung. 2018;69:97–109. https://doi.org/10.1556/018.68.2018.1.8
- 45. Sharma S, Singh VK, Kumar A, Mallubhotla S. Effect of nanoparticles on oxidative damage and antioxidant defense system in plants. Mol Plant Abiotic Stress Biol Biotechnol. 2019;315–33 https://doi.org/10.1002/9781119463665.ch17
- 46. Ramezani M, Gerami M, Majlesi Z. Comparison between various concentrations of commercial and synthesized silver nanoparticles on biochemical parameters and growth of Stevia rebaudiana. Plant Physiol Reports. 2019;24:141–52. https://doi.org/10.1007/s40502-018-0413-5
- 47. Hasan M, Sajjad M, Zafar A, Hussain R, Anjum SI, Zia M, et al. Blueprinting morpho-anatomical episodes via green silver nanoparticles foliation. Green Process Synth. 2022;11(1):697–708. https://doi.org/10.1515/gps-2022-0050
- 48. Guo H, Liu Y, Chen J, Zhu Y, Zhang Z. The effects of several metal nanoparticles on seed germination and seedling growth: a meta-analysis. Coatings. 2022;12(2):183. https://doi.org/10.3390/coatings12020183
- 49. Yadu B, Chandrakar V, Korram J, Satnami ML, Kumar M. Silver nanoparticle modulates gene expressions, glyoxalase system and oxidative stress markers in fluoride stressed Cajanus cajan L. J Hazard Mater. 2018;353:44–52. https://doi.org/10.1016/j.jhazmat.2018.03.061
- 50. Gupta SD, Agarwal A, Pradhan S. Phytostimulatory effect of silver nanoparticles (AgNPs) on rice seedling growth: An insight from antioxidative enzyme activities and gene expression patterns. Ecotoxicol Environ Saf. 2018;161:624–33. https://doi.org/10.1016/j.ecoenv.2018.06.023
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