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Plant Science Today (PST)

Research Articles

Early Access

Effects of substrates and light-emitting diode illumination on the phytochemical content of roselle (Hibiscus sabdariffa L.) microgreens

DOI
https://doi.org/10.14719/pst.13160
Submitted
11 December 2025
Published
09-03-2026

Abstract

A study was conducted to investigate the effect of substrates and illumination using light-emitting diodes on the total phenolic content (TPC) and flavonoid content (TFC) of roselle microgreens. Light-emitting diodes such as red, blue, a combination of red and blue (RB) and white were used as artificial lights of the roselle microgreens. Cocopeat, carbonized rice hull (CRH) and vermiculite are the substrates of microgreens. Newly sown seeds were exposed to 48 hr of darkness followed by light exposure. Light exposure of 10 hr was done, depending on the LEDs (red (R), blue (B), RB and white), while 14 hr of darkness until 7 days after sowing (DAS), the time of harvesting (with true leaves). Roselle microgreens were grown under ambient conditions (temperature: 28 ± 3 °C; relative humidity: 60 ± 5 %). For a fresh yield of microgreens, blue LED + CRH and red LED + vermiculite obtained the highest. Roselle microgreens grow best in RB LED + cocopeat to obtain higher TPC and TFC. The antioxidant activity was higher in roselle microgreens grown under cocopeat and vermiculite with either blue, red, or RB LEDs. The availability of the substrate may be considered when combining it with the LED, using the following combinations to obtain high TPC and TFC. For TPC, these include cocopeat + RB, CRH + RB or white and vermiculite + white. For TFC, the suitable substrates are cocopeat and CRH + RB, as well as vermiculite + white.

References

  1. 1. Baranska D, Panek J, Rozalska S, Turnau K, Frac M. Microgreens as the future of urban horticulture and superfoods, supported by post-harvest innovations for shelf-life increase: a review. Sci Hortic. 2025;350:114303. https://doi.org/10.1016/j.scienta.2025.114303
  2. 2. Ebert A. Sprouts and microgreens-novel food sources for healthy diets. Plants. 2022;11:571. https://doi.org/10.3390/plants11040571
  3. 3. Ayeni A. Nutrient content of micro/baby-green and field-grown mature foliage of tropical spinach (Amaranthus sp.) and roselle (Hibiscus sabdariffa L.). Foods. 2021;10:2546. https://doi.org/10.3390/foods10112546
  4. 4. Haena, Jindal N, Riar C. Microgreens: a comprehensive review on growth conditions, nutritional quality and their applications. Food Rev Int. 2025;1:1–33. https://doi.org/10.1080/87559129.2025.2550590
  5. 5. Kurniawan E, Ishak, Suryani. Utilization of cocopeat and goat dirt in making of solid organic fertilizer to improve macronutrient quality (NPK). IOP Conf Ser Mater Sci Eng. 2018;543:012001. https://doi.org/10.1088/1757-899X/543/1/012001
  6. 6. Gbollie S, Mwonga S, Kibe A. Effects of calcium nitrate levels and soaking durations on cocopeat nutrient content. J Agric Chem Environ. 2021;10:372–88. https://doi.org/10.4236/jacen.2021.103024
  7. 7. Philippine Rice Research Institute (PhilRice). Golden waste. 2019.
  8. 8. Pisa C, Wuta M, Muchaonyerwa P. Effects of incorporation of vermiculite on carbon and nitrogen retention and concentration of other nutrients during composting of cattle manure. Bioresour Technol Rep. 2020;100383. https://doi.org/10.1016/j.biteb.2020.100383
  9. 9. Huang Z, Zou Z, He C, He Z, Zhang Z, Li J. Physiological and photosynthetic responses of melon (Cucumis melo L.) seedlings to three Glomus species under water deficit. Plant Soil. 2011;39:391–9. https://doi.org/10.1007/s11104-010-0591-z
  10. 10. Mitchell C, Both A, Bourget M, Burr J, Kubota C, Lopez R, et al. LEDs: the future of greenhouse lighting. Chron Hortic. 2012;52:6–12.
  11. 11. Kami C, Lorrain S, Hornitschek P, Fankhauser C. Light-regulated plant growth and development. Curr Top Dev Biol. 2010;91:29–66. https://doi.org/10.1016/S0070-2153(10)91002-8
  12. 12. Kreslavskii V, Los D, Schmitt F, Zharmukhamedov S, Kuznetsov V, Allakhverdiev S. The impact of phytohormones on photosynthetic processes. Biochim Biophys Acta. 2018;1859:400–8. https://doi.org/10.1016/j.bbabio.2018.03.003
  13. 13. Murad M, Razi K, Jeong B, Samy P, Muneer S. Light-emitting diodes as agricultural lighting: impact and potential on improving physiology, flowering and secondary metabolites of crops. Sustainability. 2021;13:1985. https://doi.org/10.3390/su13041985
  14. 14. Jarerat A, Techavuthiporn C, Chanchomsuek C, Nimitkeatkai H. Enhancement of antioxidant activity and bioactive compounds in eggplants using postharvest LED irradiation. Hortic. 2022;8:134. https://doi.org/10.3390/horticulturae8020134
  15. 15. Narouei Z, Goli S, Sabzalian M, Shirvani A, Moradabbasi M. Effect of light-emitting diode irradiation on functional quality and shelf life of basil microgreens. J Essent Oil Res. 2024;6:367–79. https://doi.org/10.1080/10412905.2024.2371830
  16. 16. Lee S, Park C, Kim J, Ahn K, Kwon H, Kim J, et al. LED lights influenced phytochemical contents and biological activities in kale (Brassica oleracea L. var. acephala) microgreens. Antioxidants. 2023;12:1686. https://doi.org/10.3390/antiox12091686
  17. 17. Podsedek A, Fraszczak B, Sosnowska D, Kajszczak D, Szymczak K, Bonikowski R. LED light quality affected bioactive compounds, antioxidant potential and nutritional value of red and white cabbage microgreens. Appl Sci. 2023;13:5435. https://doi.org/10.3390/app13095435
  18. 18. Park C, Park Y, Yeo H, Kim J, Park S. Effects of light-emitting diodes on accumulation of phenolic compounds and glucosinolates in Brassica juncea sprouts. Hortic. 2020;6:77. https://doi.org/10.3390/horticulturae6040077
  19. 19. Ladeska V, Elya B, Hanafi M. Antioxidants, total phenolic and flavonoid content and toxicity assay of ampleas (Tetracera macrophylla Wall. ex Hook. F & Thoms) from Kalimantan, Indonesia. Pharmacogn J. 2022;14:642–8. https://doi.org/10.5530/pj.2022.14.147
  20. 20. Naznin M, Lefsrud M, Azad M, Park C. Effect of different combinations of red and blue LED light on growth characteristics and pigment content of in vitro tomato plantlets. Agriculture. 2019;9:196. https://doi.org/10.3390/agriculture9090196
  21. 21. Arif A, Budiyanto A, Seitawan, Cahyono T, Sulistiyani R, Marwati T, et al. Scientifica (Cairo). 2024;3815651. https://doi.org/10.1155/2024/3815651
  22. 22. Ponno J, Hoecker U. Signaling mechanisms by Arabidopsis cryptochromes. Front Plant Sci. 2022;13:844714. https://doi.org/10.3389/fpls.2022.844714
  23. 23. Gomez C, Izzo L. Increasing efficiency of crop production with LEDs. AIMS Agric Food. 2018;3:135–53. https://doi.org/10.3934/agrfood.2018.2.135
  24. 24. Kusuma P, Pattison P, Bugbee B. From physics to fixtures to food: current and potential LED efficacy. Hortic Res. 2020;7. https://doi.org/10.1038/s41438-020-0283-7
  25. 25. Fukuda N, Yoshida T, Olsen J, Senaha C, Jikukaru Y, Kamiya Y. Short main shoot length and inhibition of floral bud development under red light can be recovered by application of gibberellin and cytokinin. Acta Hortic. 2013;956:215–22. https://doi.org/10.17660/ActaHortic.2012.956.23
  26. 26. Qiao J, Li Z, Lv Z, Liu S, Chen S, Feng Y. Effects of different combinations of red and blue light on edible organ morphology and quality of buckwheat (Fagopyrum esculentum Moench) microgreens. Agriculture. 2024;14:751. https://doi.org/10.3390/agronomy14040751
  27. 27. Kong Y, Kamath D, Zheng Y. Blue versus red light can promote elongation growth independent of photoperiod: a study in four Brassica microgreens species. HortScience. 2019;54:1955–61. https://doi.org/10.21273/HORTSCI14286-19
  28. 28. Qian H, Liu T, Deng M, Miao H, Cai C, Shen W, et al. Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts. Food Chem. 2016;196:1232–8. https://doi.org/10.1016/j.foodchem.2015.10.055
  29. 29. Thwe A, Kim Y, Li X, Seo J, Kim S, Suzuki T, et al. Effects of light-emitting diodes on expression of phenylpropanoid biosynthetic genes and accumulation of phenylpropanoids in Fagopyrum tataricum sprouts. J Agric Food Chem. 2014;62:4839–45. https://doi.org/10.1021/jf501335q
  30. 30. Kim Y, Kim Y, Li X, Choi S, Park S, Park J, et al. Accumulation of phenylpropanoids by white, blue and red light irradiation and their organ-specific distribution in Chinese cabbage (Brassica rapa spp. pekinensis). J Agric Food Chem. 2015;63:6772–8. https://doi.org/10.1021/acs.jafc.5b02086

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