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Plant Science Today (PST)

Research Articles

Early Access

The role of Lactobacillus casei bacteria in improving the microbial and sensory properties of fermented millet puree

DOI
https://doi.org/10.14719/pst.3971
Submitted
24 May 2024
Published
19-05-2025

Abstract

This study aimed to prepare functional foods from local proso millet puree (from whole grains, crushed grains and millet flour) fermented with Lactobacillus casei bacteria and to follow up on its role in improving the properties of the puree. Fermentation was carried out at 37n°C for 24 and 48 hrs using (0, 2, 4, 6 %) pre-activated Lactobacillus casei in liquid MRS medium and skim milk, respectively. The results showed an increase in the number of starter bacteria with an increase in the inoculation rate and incubation time. The highest concentration was reached when using millet ground at an inoculation rate of 6 % 24 and 48 hrs after the end of fermentation, as it was 8.86 and 9.75 CFU/g. The pH dropped differently depending on how many bacteria were added and how long the fermentation took. The lowest pH was recorded when 6 % bacteria were added to the flour and after 24 hrs of fermentation, it reached 3.09. There were significant differences at this level (P≤0.05). The total phenol levels went up in both the water-based and alcohol-based extracts of the puree, with the alcohol extract showing the highest levels after the two fermentation periods. The highest levels were found in the pureed millet at a 4 % inoculation rate after 48 hrs, where they were (638.12, 801.58 µg/g), but they also went down. Following 6 % in the whole grain alcoholic extract after 24 and 48 hrs, at 524.28 and 931.72 g/g, respectively, the water-based extract of whole grain puree and crushed extract contained the highest number of tannins. The fermented flour had the lowest amount of tannin. With different inoculation rates and fermentation times, the levels of tannins increased while the levels of phytates varied. After 48 hrs, the puree of flour treatments at all inoculation rates had the highest decrease (275.1, 264.5 and 486.7 g/g, respectively), followed by the whole grain treatments at 4 % inoculation rate (264.5 and 624.2 g/g) respectively. Finally, all the purees in general achieved the highest sensory ratings and general acceptance compared to the control treatments, with significant differences (P< 0.05) among the many sensory attributes used in sensory evaluation.

References

  1. 1. Al-mhyawi EKH. Studying the physiochemical and qualitative properties of cereal and flour of local and imported millet and preparing gluten free bread. A dissertation submitted to the council of college of agriculture engineering sciences. University of Baghdad in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Agriculture / Food Sciences.2023: 168-240.
  2. 2. Nashmi RJ, Naser JM. Improving the rheological and qualitative properties of bread wheat by barley-extracted pentosanes. Iraqi Journal of Agricultural Sciences. 2022; 53(5):1212-22 . ‏https://doi.org/10.36103/ijas.v53i5.1635.
  3. 3. Al-Mhyawi EK, Nasser JM. Physical and Functional Properties of some Millet Cultivars in Iraq. InIOP Conference Series: Earth and Environmental Science 2023: IOP Publishing. . https://doi.org/10.1088/1755-1315/1225/1/012036‏
  4. 4. Wang H, Fu Y, Zhao Q, Hou D, et al. Effect of different processing methods on the millet polyphenols and their anti-diabetic potential. Frontiers in nutrition. 2022; 9:780499. . https://doi.org/10.3389/fnut.2022.780499
  5. 5. Hassan ZM, Sebola NA, Mabelebele M. The nutritional use of millet grain for food and feed: a review. Agriculture & food security. 2021; 10:1-4. .‏ https://doi.org/10.1186/s40066-020-00282-6
  6. 6. Hussain LA, Hamdalla MS, Yousif SA. Study baking quality of some bread wheat genotypes under water stress. Iraqi Journal of Agricultural Sciences. 2023 Aug; 54(4):996-07. .‏ https://doi.org/10.36103/ijas.v54i4.1788.
  7. 7. Seo MC, Ko JY, Song SB, Lee JS, Kang JR, et al. Antioxidant compounds and activities of foxtail millet, proso millet and sorghum with different pulverizing methods. Journal of the Korean Society of Food Science and Nutrition. 2011; 40(6):790-97. https://doi.org/10.3746/jkfn.2011.40.6.790
  8. 8. Singh M. Physico-chemical, rheological and baking properties of proso millet.‏ A thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in Bio systems and Agricultural Engineering in the College of Engineering at the University of Kentucky.2016.
  9. 9. Ilango S, Antony U. Assessment of the microbiological quality of koozh, a fermented millet beverage. African Journal of Microbiology Research. 2014: 8(3):308-12.‏ https://doi.org/10.5897/AJMR2013.6482
  10. 10. Jasim AS, Nasser JM. The functional properties of chemically and enzymatically modified wheat gluten. Iraqi Journal of Agricultural Sciences. 2023: 54(5):1279-91. https://doi.org/10.36103/ijas.v54i5.1826.
  11. 11. Jassim Al-khafagi MF, Mohammed DY. Comparison phytochemical compounds from two different solvents of crude capparis spinosa extracts. Iraqi Journal of Agricultural Sciences. 2023; 54(5):1234-42..‏https://doi.org/10.36103/ijas.v54i5.1818.
  12. 12. Balli D, Cecchi L, Pieraccini G, Venturi M, Galli V, et al. Millet fermented by different combinations of yeasts and lactobacilli: Effects on phenolic composition, starch, mineral content and prebiotic activity. Foods. 2023; 12(4):748. .‏ https://doi.org/10.3390/foods12040748
  13. 13. Manufactured from local mills flour. Iraqi Journal of Science, 1920-27. ‏
  14. 14. Fasreen MM, Perera OD, Weerahewa HL. Development of finger millet based probiotic beverage using Lactobacillus casei431.2017. https://doi.org/10.4038/ouslj.v12i1.7384‏
  15. 15. Dar BN, Savita Sharma SS. Total phenolic content of cereal brans using conventional and microwave assisted extraction.. American Journal of Food Technology2011: 6(12), 1045-53.‏ https://doi.org/10.3923/ajft.2011.1045.1053
  16. 16. Nasser JM, Hammood EK. Effect of natural phytase, fermentation and baking processes on phytate degradation in wheat bread manufactured from local mills flour. Iraqi Journal of Science. 2019:1920-27.‏ https://doi.org/10.24996/ijs.2019.60.9.4
  17. 17. Al-Mahyawi IA. Phytase enzyme of wheat in reducing the level of phytate in bread and biscuits manufactured from whole wheat flour (Doctoral dissertation, master thesis, College of Agriculture, University of Baghdad). Arora S, Jood S, Khetarpaul N, Goyal R. Effect of germination and fermentation on ph, titratable acidity and chemical composition of pearl millet based food blends. Acta alimentaria. 2009;38(1):107-15.‏ https://doi.org/10.1556/AAlim.2008.0024
  18. 18. AL-roomi FF. Y..Utilization of Quinoa Seeds (Chenopodium Quinoa Willd) as Prebiotic in Therapeutic Fermented Milk. A Thesis of M.sc. in Food Science. In The Department of Food Science. Agricultural Engineering Sciences. Baghdad University. Iraq.2021:59-60.
  19. 19. Erkmen O, Bozoglu TF. Food Microbiology: Principles into Practice. John Wiley & Sons.‏2016. https://doi.org/10.1002/9781119237860
  20. 20. Yadav H, Jain S, Rastamanesh R, Bomba A, Catanzaro R, Marotta F. Fermentation technology in the development of functional foods for human health: where we should head. Ferment. Technol. 2011; 1:1-2.
  21. 21. Thirumangaimannan G, Gurumurthy K. A study on the fermentation pattern of common millets in Koozh preparation-a traditional South Indian food. 2013.
  22. 22. Dar BN, Savita Sharma SS. Total phenolic content of cereal brans using conventional and microwave assisted extraction. 2011. https://doi.org/10.3923/ajft.2011.1045.1053‏
  23. 23. Osman MA. Effect of traditional fermentation process on the nutrient and antinutrient contents of pearl millet during preparation of Lohoh. Journal of the Saudi Society of Agricultural Sciences. 2011; 10(1):1-6.‏ https://doi.org/10.1016/j.jssas.2010.06.001
  24. 24. Bora P, Das P, Bhattacharyya R, Saikia A. Effect of processing on the phytochemical content and antioxidant capacity of proso millet (Panicum miliaceum L.) milled fractions. International Journal of Chemical Studies. 2018; 6(4):18-22.

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