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Plant Science Today (PST)

Research Articles

Vol. 12 No. 1 (2025)

Botanical and SFE aided need base systemic scientific inaugurate of Delphinium denudatum root Wall. an unexplored jeopardise medicinal plant

DOI
https://doi.org/10.14719/pst.4249
Submitted
3 July 2024
Published
10-01-2025 — Updated on 12-01-2025
Versions

Abstract

The endangered medicinal plant Delphinium denudatum Wall. is widely recognized by the medical community for its potential in treating neurological disorders. Despite its known health benefits, it has not been thoroughly investigated. Further research is required using scientifically recognized analytical techniques to fully explore its therapeutic potential.
The use of traditional methods for extracting botanicals raises concerns about both environmental damage and potential health risks due to the extensive use of organic solvents. In response to these issues, this research utilizes environmentally-conscious and eco-friendly methods for botanical extraction. Recently, supercritical fluid extraction (SFE) has emerged as a preferred technique for extracting plant material due to its high efficiency and yield. Carbon dioxide (CO2) is the solvent of choice in SFE because of its effective diffusion into plant material at low temperatures, as well as its environmentally friendly properties.
In this study, the optimal conditions for SFE were determined to be a pressure of 200 bar, a temperature of 80 °C and a CO2 flow rate of 15 g. min-1 to achieve to achieve maximum yield of Delphinium root extract (DRE). The result demonstrated that SFE produced an optimal extract yield with three productivity levels: level-I (0.8 % w/w), level-II (1.2 % w/w) and level-III (0.95 % w/w). Furthermore, a botanical examination verified the integrity of cell structures, while physicochemical evaluations help establish quality standards for the extract.
Phytometabolites mapping of the SFE- derived DRE was performed using HPTLC under various conditions, including daylight, postderivatization and at 254 nm and 366 nm. Additionally, community reports validated the effectiveness of DDR against harmful microorganisms, with zones of inhibition observed against Staphylococcus aureus (ZI: 5.21 ± 0.29); Pseudomonas aeruginosa (ZI: 7.48 ± 0.53); Aspergillus niger (ZI: 8.15 ± 0.9) at concentrations of 100 ?g mL-1 and 500 ?g mL-1 of the DRE respectively.
In conclusion, this research strongly supports the use of D. denudatum extracts obtained via SFE as a potential treatment for various pathogenic disease. To the best of our knowledge, there is no previous scientific evidence documenting the successful extraction of D. denudatum using SFE. Additionally, the protective properties of SFE-derived DRE against pathogenic microorganisms had not been previously explored. Thus, the present study identifies D. denudatum as a promising candidate for the development of drugs to combat pathogenic infections.

References

  1. Sookying S, Duangjai A, Saokaew S, Phisalprapa P. Botanical aspects, phytochemicals and toxicity of Tamarindus indica leaf and a systematic review of antioxidant capacities of T. indica leaf extracts. Front Nutr. 2022;20(9):977015. https:// doi.org/10.3389/fnut.2022.977015
  2. Mahendran G, Rahman LU. Ethnomedicinal, phytochemical and pharmacological updates on peppermint (Mentha piperita)- a review. Phytother Res. 2020;34:2088-139.
  3. https:// doi.org/10.1002/ptr.6664
  4. Yan Y, Jiang H, Yang X, Ding Z, Yin T. Grandiflolines A-F, new anti -inflammatory diterpenoid alkaloids isolated from Delphinium grandiflorum. Front Chem. 2022; 19(10):1012874. https:// doi.org/10.3389/fchem.2022.1012874
  5. Wang WJ, Zha B, Zhao JY. Three new diterpenoid alkaloids from Delphinium naviculare var. lasiocarpum. Phytochem Lett. 2019;33:12-16. https://doi.org/10.1016/j.phytol.2019.06.009
  6. Arunachalam K, Parimelazhagan T. Antioxidant and antimicrobial potential of methanolic extract of Indian sacred grove Gymnostachyum febrifugum Benth. root. IJPBR. 2011;2(3):67-71.
  7. Gupta AK, K Mahfoozurrahman, Danish K. Physicochemical study of Delphinium denudatum Wall. (Ranunculales: Ranunculaceae) and their antioxidant activity. Braz J Biol Sci. 2019;6 (12):161-69. https://doi.org/10.21472/bjbs.061215
  8. Gopalakrishnan K, Udayakumar R. GC-MS analysis of phytocompounds of leaf and stem of Marsilea quadrifolia (L). Int J Biochem Res Rev. 2014;4(6):517-26. https://doi.org/10.9734/ IJBCRR/2014/11350
  9. Hassim N, Markom M, Rosli MI, Harun S. Scale-up approach for supercritical fluid extraction with ethanol-water modified carbon dioxide on Phyllanthus niruri for safe enriched herbal extracts. Sci Rep. 2021;4:11(1):15818. https://doi.org/10.1038/ s41598-021-95222-0
  10. Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med J. 2018;13:20. https://doi.org/10.1186/s13020-018-0177-x
  11. Osorio-Tobon JF. Recent advances and comparisons of conventional and alternative extraction techniques of phenolic compounds. J Food Technol. 2020;57:4299-315. https:// doi.org/10.1007/s13197-020-04433-2
  12. Pronyk C, Giuseppe M. Design and scale-up of pressurized fluid extractors for food and bioproducts. J Food Eng. 2009;95:215- 26. https://doi.org/10.1016/j.jfoodeng.2009.06.002
  13. Montesantos N, Maschietti M. Supercritical carbon dioxide extraction of lignocellulosic bio-oils: The potential of fuel upgrading and chemical recovery. Energies. 2020;13:1600. https:// doi.org/10.3390/en13071600
  14. Hu H, Zhang J, Chen G. Extraction of Huadian oil shale with water in sub-and supercritical states. Fuel. 1999;78(6):645-51. https://doi.org/10.1016/S0016-2361(98)00199-9
  15. Norodin NSM, Salleh LM, Mustafa NM. Supercritical carbon dioxide (SC-CO2) extraction of essential oil from Swietenia mahagoni seeds. Mater Sci Eng. 2016;162:12-30. https:// doi.org/10.1088/1757-899X/162/1/012030
  16. Krakowska-Sieprawska A, Rafi?ska K, Walczak-Skierska J, Kie?basa A, Buszewski B. Promising green technology in obtaining functional plant preparations: Combined enzyme-assisted supercritical fluid extraction of flavonoids isolation from Medicago sativa leaves. Materials (Basel). 2021;14 (11):2724. https://doi.org/10.3390/ma14112724
  17. Tyskiewicz K, Konkol M, Roj E. The application of supercritical fluid extraction in phenolic compounds isolation from natural plant materials. Molecules. 2018;23(10):2625. https:// doi.org/10.3390/molecules23102625
  18. da Silva CG, Lucas AM, Santo ATDE, Almeida RN, Cassel E, Vargas RM. Sequential processing of Psidium guajava L. leaves: steam distillation and supercritical fluid extraction. Braz J Chem Eng. 2019;36(1):487-96. https://doi.org/10.1590/0104 6632.20190361s20170215
  19. Suleiman WB. In-vitro estimation of supercritical fluid extracts of some plants for their antimicrobial potential, phytochemistry and GC-MS analyses. Ann Clin Microbiol Antimicrob. 2020;19:29. https://doi.org/10.1186/s12941-020-00371-1
  20. Chen Y, Zhang X, Guo Q. Plant morphology, physiological characteristics, accumulation of secondary metabolites and antioxidant activities of Prunella vulgaris under UV solar exclusion. J Biol Res. 2019;52:17. https://doi.org/10.1186/s40659-019-0225-8
  21. Cabi E, Dogan M, Mavi O, Karabacak E, Baser B. Elymus sos-nowskyi (Hackel) Melderis (Poaceae), a rare endemic species in Turkey. Turk J Bot. 2010;34(2):105-14. https://doi.org/10.3906/bot-0902-4
  22. Harborne JB. Phytochemical methods. Chapman and Hall Ltd., London. 1973. p. 49-188.
  23. Mushtaq A, Akbar S, Zargar MA, Wali AF, Malik AH, Dar MY, et al. Phytochemical screening, physicochemical properties, acute toxicity testing and screening of hypoglycaemic activity of ex-tracts of Eremurus himalaicus baker in normoglycaemic Wistar strain albino rats. Biomed Res Int. 2014;867547. https://doi.org/10.1155/2014/867547
  24. Mukherjee PK. Quality control of herbal drugs. 1st Edition, Busi-ness Horizon, Pharmaceutical Publishers, New Delhi. 2002; p. 380-422.
  25. Daoud A, Malika D, Bakari S, Hfaiedh N, Mnafgui K, Kadri A, Ghar-sallah N. Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of date palm pollen (DPP) from two Tunisian cultivars. Arab J Chem. 2019;12(8):3075-86. https://doi.org/10.1016/j.arabjc.2015.07.014
  26. Zhao ZG, Yan HF, Zheng R, Saeed K, Fu M, Tao X, Zhang ZY. An-thocyanins characterization and antioxidant activities of sugar-cane (Saccharum officinarum L.) rind extracts. Ind Crops Prod. 2018;113:38-45. https://doi.org/10.1016/J.INDCROP.2018.01.015
  27. Mantena VRKR, Soni D, Rao GVB. Anti asthmatic activity of meth-anolic extract of Delphinium denudatum Wall. Int J Pharm Sci. 2011; 3(4):167-70.
  28. Werff HVD, Consiglio T. Distribution and conservation signifi-cance of endemic species of flowering plants in Peru. Int J Bio-divers. 2004;13(9):1699-713. https://doi.org/10.1023/B:BIOC.0000029334.69717.f0
  29. El Aziz MMA, Ashour AS, Melad ASG. A review on saponins from medicinal plants; chemistry, isolation and determination. J Nanomed Res. 2019;7(4):282-88. https://doi.org/10.1 5406/jnmr.2019.07.00199
  30. Passos CP, Silva RM, Silva FAD, Coimbra MA, Silva CM. Enhance-ment of the supercritical fluid extraction of grape seed oil by using enzymatically pre-treated seed. J Supercrit Fluids. 2009;48:225-29. https://doi.org/10.1016/j.supflu.2008.11.001
  31. Nagavekar N, Singhal RS. Enhanced extraction of oleoresin from Piper nigrum by supercritical carbon dioxide using ethanol as a co-solvent and its bioactivity profile. J Food Process Eng. 2018;41:12670. https://doi.org/10.1111/jfpe.12670
  32. Karami Z, Emam-Djomeh Z, Mirzaee HA, Khomeiri M, Mahoonak AS, Aydani E. Optimization of microwave assisted extraction (MAE) and Soxhlet extraction of phenolic compound from lico-rice root. J Food Technol. 2015;52:3242-53. https://doi.org/10.1007/s13197-014-1384-9
  33. Vatai T, Skerget M, Knez Z. Extraction of phenolic compounds from elder berry and different grape marc varieties using organ-ic solvents and/or supercritical carbon dioxide. J Food Eng. 2009;90:246-54. https://doi.org/10.1016/j.jfoodeng.2008.06.028
  34. Sun Q, Shi J, Scanlon M, Xue SJ, Lu J. Optimization of supercriti-cal CO2 process for extraction of tocopherol rich oil from Canola seeds. LWT Food Science and Technology. 2021; 145:111435. https://doi.org/10.1016/j.lwt.2021.111435
  35. Dumont MJ, Narine SS. Soap stock and deodorizer distillates from North American vegetable oils: Review on their characteri-zation, extraction and utilization. Int Food Res J. 2007;40(8):957 957-74. https://doi.org/10.1016/j.foodres. 2007.06.006
  36. Fikselova M, Silhar S, Marecek J, Francakova H. Extraction of carrot (Daucus carota L.) carotenes under different conditions. Czech J Food Sci. 2008; 26:268-74. https://doi.org/10.17221/9/2008-CJFS
  37. Mustafa A, Turner C. Pressurized liquid extraction as a green approach in food and herbal plants extraction: A review. Anal Chim Acta. 2011;703:8-18. https://doi.org/10.1016/j.aca.2011.07.018
  38. Sattler R. Kaplan’s principles of plant morphology: A critical review. Bot Rev. 2022;88:257-70. https://doi.org/10.1007/s12229-022-09280-8
  39. Martinez-Gomez J, Atluri TAM, Rose IJ, Holliday AJ, Strock CF, Lync JP, et al, Developmental morphology and anatomy shed light on both parallel and convergent evolution of the umbellate inflorescence in monocots, underlain by a new variant of metatopy. Front Plant Sci. 2022;13:873505. https://doi.org/10.5281/zenodo.6012529
  40. Turabekova MA, Rasulev BF, Dzhakhangirov FN, Toropov AA, Leszczynska D, Leszczynski J. Aconi-tum and Delphinium diterpenoid alkaloids of local anaesthetic activity: Comparative QSAR analysis based on GA-MLRA/PLS and optimal descriptors approach. Environ Carcinog Ecotoxicol Rev. 2014;32:213-38. https://doi.org/10.1080/10590501.2014.938886
  41. Orellana MR, Lopez-Pujol J, Blanche C. Genetic diversity in Delphinium staphisagria (Ranunculaceae), a rare Mediterrane-an dysploid larkspur with medicinal uses. Genetica. 2009; 135:221-32. https://doi.org/10.1007/s10709-008-9271-9
  42. Utelli AB, Roy BA, Baltisberger M. Molecular and morphological analyses of European aconitum species (Ranunculaceae). Pl Syst Evol. 2000;224(3):195212. https://doi.org/10.3390/molecules23102625
  43. Diaz JG, Herz W. Acylated flavonol tetraglycosides from Delphin-ium gracile. Phytochemistry. 2000;71(4):463-68. https://doi.org/10.1016/j.phytochem.2009.11.011
  44. Rahman A, Nasreen A, Akhtar F, Shekhani MS, Clardy J, Parvez M, Choudhary MI. Antifungal diterpenoid alkaloids from Delphin-ium denudatum Wall. J Nat Prod. 1997;60(5):472-74. https://doi.org/10.1021/np960663n
  45. Bhat BA, Mir WR, Sheikh BA, Rather MA, Dar TH, Mir MA. In-vitro and in-silico evaluation of antimicrobial properties of Delphini-um cashmerianum L., a medicinal herb growing in Kashmir, India. J Ethnopharmacol. 2022;291:115046. https://doi.org/10.1016/j.jep.2022.115046
  46. Zafar S, Ahmad MA, Siddiqui TA. Protective role of Delphinium denudatum (Jadwar) against morphine induced tolerance and dependence in Mice. J Ethnopharmacol. 2001; 78:95-98. https://doi.org/10.1016/s0378-8741(01)00317-8
  47. Amina M, Musayeib Al, Alarfaj NM, El-Tohamy NA, Al-Hamoud MF, Alqenaei GA. The fluorescence detection of phenolic com-pounds in Plicosepalu scurviflorus extracts using biosynthesized ZnO nanoparticles and their biomedical potential. Plant. 2022;11:361. https://doi.org/10.3390/plants11030361
  48. Prakash A, Janmeda P, Pathak P. Development and standardi-zation of quality control parameters of different parts of Trian-thema portulacastrum L.. SN Appl Sci. 2019;1:1108. https://doi.org/10.1007/42452-019-1074-3
  49. Dave R, Nagani K, Chanda S. Pharmacognostic studies and physicochemical properties of the Polyalthia longifolia var. pen-dula leaf. Pharmacogn Mag. 2010;2:572-76. https://doi.org/10.1016/S0975-3575 (10)80062-8
  50. Mercy LS, Gnanamani A, Ilavarasan R. Physicochemical, phyto-chemical and high performance thin layer chromatography analysis of the whole plant of Orthosiphon thymiflorus (Roth.) Sleesen. Asian J Pharm Clin Res. 2015;8(1):181-84.
  51. Yin T, Cai L, Ding Z. An overview of the chemical constituents from the genus Delphinium reported in the last four decades. R Soc Chem. 2020;10:13669-86. https://doi.org/10.1039/D0RA00813C

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