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Plant Science Today (PST)

Review Articles

Vol. 12 No. 2 (2025)

Sustainable strategies to combat anti-nutrition in legumes: Implications for global food security

DOI
https://doi.org/10.14719/pst.5485
Submitted
2 October 2024
Published
31-05-2025 — Updated on 10-06-2025
Versions

Abstract

Vegetables are indispensable components of a balanced diet, providing essential nutrients for overall health and well-being. In Indian cuisine, where vegetables typically constitute approximately 20 % of dietary intake, their significance is further highlighted by leguminous vegetables, which play a critical role in addressing protein malnutrition. Despite their beneficial nutritional content, leguminous vegetables contain bioactive compounds known as anti-nutritional factors (ANFs), which can have a negative impact on human health. These ANFs include substances that either reduce nutrient availability or have toxic effects when consumed. To mitigate the adverse effects of ANFs, various traditional methods, including milling, soaking, boiling, thermal processing, sprouting, fermentation, and genetic modification, have been effectively employed. Additionally, modern techniques such as pulsed electric fields (PEF), ultrasonic treatment, and high hydrostatic pressure (HHP) are being increasingly applied to neutralize ANF activity. This review emphasises the essential role of leguminous vegetables in the Indian diet, the various challenges posed by anti-nutritional factors, and the range of both traditional and modern strategies developed to maximize nutrient retention while minimizing potential health risks.

References

  1. 1. Kader AA, Perkins-Veazie P, Lester GE. Nutritional quality of fruits, nuts, and vegetables and their importance in human health. USDA Handbook. 2004;66.
  2. 2. Wink M. Evolution of secondary metabolites in legumes (Fabaceae). South African J Bot. 2013;89:164–75. https://doi.org/10.1016/j.sajb.2013.06.006
  3. 3. Varshney RK, Mohan SM, Gaur PM, Gangarao NV, Pandey MK, Bohra A, et al. Achievements and prospects of genomics-assisted breeding in three legume crops of the semi-arid tropics. Biotech adv.2013;31(8):1120–34. https://doi.org/10.1016/j.biotechadv.2013.01.001
  4. 4. Boschin G, Arnoldi AJFC. Legumes are valuable sources of tocopherols. Food Chem. 2011;127(3):1199–203. https://doi.org/10.1016/j.foodchem.2011.01.124
  5. 5. Bouchenak M, Lamri-Senhadji M. Nutritional quality of legumes and their role in cardiometabolic risk prevention: a review. J Medi food. 2013;16(3):185–98. https://doi.org/10.1089/jmf.2011.0238
  6. 6. Becerra-Tomás N, Díaz-López A, Rosique-Esteban N, Ros E, Buil-Cosiales P, Corella D, et al. Legume consumption is inversely associated with type 2 diabetes incidence in adults: A prospective assessment from the PREDIMED study. Clinical Nutr. 2018;37(3):906–13. https://doi.org/10.1016/j.clnu.2017.03.015
  7. 7. Singh B, Singh JP, Kaur A, Singh N. Phenolic composition and antioxidant potential of grain legume seeds: A review. Food Res Int. 2017;101:1–16. https://doi.org/10.1016/j.foodres.2017.09.026
  8. 8. Sabaté J, Wien mjbjon. A perspective on vegetarian dietary patterns and risk of metabolic syndrome. British J Nutrition. 2015;113(S2):S136–S43. https://doi.org/10.1017/S0007114514004139
  9. 9. Shimelis EA, Rakshit SKJL-FS, Technology. Proximate composition and physico-chemical properties of improved dry bean (Phaseolus vulgaris L.) varieties grown in Ethiopia. LWT-Food Sci Tech. 2005;38(4):331–38. https://doi.org/10.
  10. 1016/j.lwt.2004.07.002
  11. 10. Abara AE. Tannin content of Dioscorea bulbufera. Abara AE. J Chem Soc Nigeria. 2003;28:55–56.
  12. 11. Samtiya M, Aluko RE, Dhewa TJFP, Processing, Nutrition. Plant food anti-nutritional factors and their reduction strategies: An overview. Food Prod Pro Nut. 2020;2:1–14. https://doi.org/10.1186/s43014-020-0020-5
  13. 12. Soetan K, Oyewole OJAJofs. The need for adequate processing to reduce the anti-nutritional factors in plants used as human foods and animal feeds: A review. African J Food Sci. 2009;3(9):223–32.
  14. 13. Zhang J, Shi J, Ilic S, Jun Xue S, Kakuda YJFRI. Biological properties and characterization of lectin from red kidney bean (Phaseolus vulgaris). Food Rev Int. 2008;25(1):12–27. https://doi.org/10.1080/87559120802458115
  15. 14. Naithani S, Komath SS, Nonomura A, Govindjee G. Plant lectins and their many roles: Carbohydrate-binding and beyond. J Plant Physiol. 2021;266:153531. https://doi.org/10.1016/j.jplph.2021.153531
  16. 15. Sauvion N, Nardon C, Febvay G, Gatehouse AM, Rahbé YJJoIP. Binding of the insecticidal lectin Concanavalin A in pea aphid, Acyrthosiphon pisum (Harris) and induced effects on the structure of midgut epithelial cells. 2004;50(12):1137–50. https://doi.org/10.1016/j.jinsphys.2004.10.006
  17. 16. Giri AP, Kachole MS. Amylase inhibitors of pigeon pea (Cajanus cajan) seeds. Phytochemistry. 1998;47(2):197–202. https://doi.org/10.1016/S0031-9422(97)00570-0
  18. 17. Mohanto K, Aye AT. An analysis of health risk and potential elimination strategies of anti-nutritional factors in cereals and legumes. Asian Food Sci J. 2024;23:44–51. https://doi.org/10.9734/afsj/2024/v23i7725
  19. 18. Fernando R, Pinto M, Pathmeswaran A. Goitrogenic food and prevalence of goitre in Sri Lanka. Int J Int Med. 2012;1(2):17–20.
  20. 19. Thakur A, Sharma V, Thakur A. An overview of anti-nutritional factors in food. Int J Chem Stud. 2019;7(1):2472–79.
  21. 20. Ramadoss BR, Shunmugam AS. Anti-dietetic factors in legumes-local methods to reduce them. Int J Food Nutr Sci. 2014;3(3):84–89.
  22. 21. Akande KE, Doma UD, Agu H, Adamu H. Major antinutrients found in plant protein sources: their effect on nutrition. Pak J Nutr. 2010;9(8):827–32. https://doi.org/10.3923/pjn.2010.827.832
  23. 22. Banti M, Bajo W. Review on nutritional importance and anti-nutritional factors of legumes. Int J Food Sci Nutr. 2020;9(13):8–49. https://doi.org/10.11648/j.ijnfs.20200906.11
  24. 23. Sparg S, Light M, Van Staden J. Biological activities and distribution of plant saponins. J Ethnopharmacol. 2004;94(2-3):219–43. https://doi.org/10.1016/j.jep.2004.05.016
  25. 24. Loewus FA. Biosynthesis of phytate in food grains and seeds. Food phytates: CRC Press; 2001. p. 69–78. https://doi.org/10.1201/9781420014419.ch4
  26. 25. Shi L, Arntfield SD, Nickerson M. Changes in levels of phytic acid, lectins and oxalates during soaking and cooking of Canadian pulses. Food Res Int. 2018;107:660–68. https://doi.org/10.1016/j.foodres.2018.02.056
  27. 26. Simee W. Isolation and determination of antinutritional compounds from root to shells of peanut (Arachis Hypogaea). J Disper Sci Technol. 2011;28:341–47.
  28. 27. Margier M, Georgé S, Hafnaoui N, Remond D, Nowicki M, Du Chaffaut L, et al. Nutritional composition and bioactive content of legumes: Characterization of pulses frequently consumed in France and effect of the cooking method. Nutrients. 2018;10(11):1668. https://doi.org/10.3390/nu10111668
  29. 28. Kuo Y-H, Rozan P, Lambein F, Frias J, Vidal-Valverde C. Effects of different germination conditions on the contents of free protein and non-protein amino acids of commercial legumes. Food chemistry. 2004;86(4):537–45. https://doi.org/10.1016/j.foodchem.2003.09.042
  30. 29. Trovato M, Funck D, Forlani G, Okumoto S, Amir R. Amino acids in plants: Regulation and functions in development and stress defense. Frontiers Media SA; 2021. p. 772810. https://doi.org/10.3389/978-2-88971-842-49
  31. 30. Greiner R, Konietzny U. Phytase for food application. Food Sci Biotechnol. 2006;44(2).
  32. 31. Dost K, Tokul O. Determination of phytic acid in wheat and wheat products by reverse phase high-performance liquid chromatography. Analytica Chimica Acta. 2006;558(1-2):22–27. https://doi.org/10.1016/j.aca.2005.11.035
  33. 32. Shamsuddin AM. Anti‐cancer function of phytic acid. Int J Food Sci Technol. 2002;37(7):769–82. https://doi.org/
  34. 10.1046/j.1365-2621.2002.00620.x
  35. 33. Golden MH. Proposed recommended nutrient densities for moderately malnourished children. Food Nutr Bull. 2009;30(3_suppl3):S267–S342. https://doi.org/10.1177/15648265090303S302
  36. 34. Bhandari MR, Kawabata J. Cooking effects on oxalate, phytate, trypsin and α-amylase inhibitors of wild yam tubers of Nepal. J Food Compost Anal. 2006;19(6-7):524–30. https://doi.org/10.1016/j.jfca.2004.09.010
  37. 35. Rao G, Shrivastava S. Toxic and antinutritional factors of new varieties of pea seeds. 2011;512–23.
  38. 36. Adeyemo S, Onilude A. Enzymatic reduction of anti-nutritional factors in fermenting soybeans by Lactobacillus plantarum isolates from fermenting cereals. Nigerian Food J. 2013;31(2):84–90. https://doi.org/10.1016/S0189-7241(15)30080-1
  39. 37. Liener I. Plant antinutritional factors| detoxification. 2003: 4587–93. https://doi.org/10.1016/B0-12-227055-X/00936-6
  40. 38. Liener IE. Phytohemagglutinins. Their nutritional significance. J Agric Food Chem. 1974;22(1):17–22. https://doi.org/10.1021/jf60191a031
  41. 39. Harold MJS, New York, NY. On food and cooking: the science and lore of the kitchen. 2004. https://doi.org/10.
  42. 1021/jf60191a031
  43. 40. Osada K. Effect of dietary apple polyphenol on metabolic disorder of lipid in rats given oxidized cholesterol. InProc. Jpn. Conf. Biochem. Lipids. 1997;39:317–20.
  44. 41. Adeyemo S, Onilude AJNFJ. Enzymatic reduction of anti-nutritional factors in fermenting soybeans by Lactobacillus plantarum isolates from fermenting cereals. Nigerian food J. 2013;31(2):84–90.https://doi.org/10.1016/S0189-7241(15)30080-1
  45. 42. Helsper JP, Hoogendijk JM, van Norel A, Burger-Meyer K. Antinutritional factors in faba beans (Vica faba L.) as affected by breeding toward the absence of condensed tannins. J Agric Food Chem. 1993;41(7):1058–61. https://doi.org/10.1021/jf00031a008
  46. 43. Zagrobelny M, Bak S, Rasmussen AV, Jørgensen B, Naumann CM, Møller BL. Cyanogenic glucosides and plant-insect interactions. Phytochemistry. 2004;65(3):293–306. https://doi.org/10.1016/j.phytochem.2003.10.016
  47. 44. Sinha K, Khare V. Review on: Antinutritional factors in vegetable crops. Pharma Innov. 2017;6(12):353–58.
  48. 45. Abbas Y, Ahmad A. Impact of processing on nutritional and antinutritional factors of legumes: a review. Ann, Food Sci Technol. 2018;19(2).
  49. 46. Ali A, Devarajan S, Manickavasagan A, Ata A. Antinutritional factors and biological constraints in the utilization of plant protein foods. Plant protein foods: Springer; 2022:407–38. https://doi.org/10.1007/978-3-030-91206-2_14
  50. 47. Gupta RK, Gangoliya SS, Singh NK. Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains. J Food Biosci Technol. 2015;52:676–84. https://doi.org/10.1007/s13197-013-0978-y
  51. 48. Vidal-Valverde C, Frias J, Estrella I, Gorospe MJ, Ruiz R, Bacon J. Effect of processing on some antinutritional factors of lentils. J Agric Food Chem. 1994;42(10):2291–95. https://doi.org/10.1021/jf00046a039
  52. 49. Kumari S. The effect of soaking almonds and hazelnuts on Phytate and mineral concentrations: University of Otago; 2018.
  53. 50. Kumar S, Verma AK, Das M, Jain S, Dwivedi PD. Clinical complications of kidney bean (Phaseolus vulgaris L.) consumption. Nutrition. 2013;29(6):821–27. https://doi.org/10.1016/j.nut.2012.11.010
  54. 51. Coulibaly A, Kouakou B, Chen Jie CJ. Phytic acid in cereal grains: structure, healthy or harmful ways to reduce phytic acid in cereal grains and their effects on nutritional quality. 2011. https://doi.org/10.3923/ajpnft.2011.1.22
  55. 52. Shimelis EA, Rakshit SK. Effect of processing on antinutrients and in vitro protein digestibility of kidney bean (Phaseolus vulgaris L.) varieties grown in East Africa. Food chemistry. 2007;103(1):161–72. https://doi.org/10.1016/j.foodchem.2006.08.005
  56. 53. Nkhata SG, Ayua E, Kamau EH, Shingiro JB. Fermentation and germination improve the nutritional value of cereals and legumes through the activation of endogenous enzymes. Food Sci Nutr. 2018;6(8):2446–58. https://doi.org/10.
  57. 1002/fsn3.846
  58. 54. Yoshiara LY, Mandarino JMG, Carrão-Panizzi MC, Madeira TB, da Silva JB, de Camargo AC,et al. Germination changes the isoflavone profile and increases the antioxidant potential of soybean. 2018. https://doi.org/10.31665/
  59. JFB.2018.3157
  60. 55. de Camargo AC, Favero BT, Morzelle MC, Franchin M, Alvarez-Parrilla E, de la Rosa LA, et al. Is chickpea a potential substitute for soybean? Phenolic bioactives and potential health benefits. Int J Mol Med Sci. 2019;20(11):2644. https://doi.org/10.3390/ijms20112644
  61. 56. Cardador‐Martínez A, Maya‐Ocaña K, Ortiz‐Moreno A, Herrera‐Cabrera BE, Dávila‐Ortiz G, Múzquiz M, et al. Effect of Roasting and Boiling on the Content of Vicine, Convicine and L‐3, 4‐dihydroxyphenylalanine in Vicia faba L. J Food Qual. 2012;35(6):419–28. https://doi.org/10.1111/jfq.12006
  62. 57. Pedrosa MM, Guillamón E, Arribas C. Autoclaved and extruded legumes as a source of bioactive phytochemicals: A review. Foods. 2021;10(2):379. https://doi.org/10.3390/foods10020379
  63. 58. Verma DK, Thakur M, Singh S, Tripathy S, Sandhu KS, Kaur M, et al. The Emphasis of Effect of Cooking and Processing Methods on Antinutritional Phytochemical of Legumes and Their Significance in Human Health. Phytochemicals in Food and Health: Apple Academic Press; 2021. p. 3–47.
  64. 59. Arbab Sakandar H, Chen Y, Peng C, Chen X, Imran M, Zhang H. Impact of fermentation on antinutritional factors and protein degradation of legume seeds: A review. Food Reviews International. 2023;39(3):1227–49. https://doi.org/10.1080/87559129.2021.1931300
  65. 60. Meinlschmidt P, Ueberham E, Lehmann J, Schweiggert-Weisz U, Eisner P. Immunoreactivity, sensory and physicochemical properties of fermented soy protein isolate. Food chemistry. 2016;205:229–38. https://doi.org/10.1016/j.foodchem.2016.03.016
  66. 61. Coda R, Varis J, Verni M, Rizzello CG, Katina K. Improvement of the protein quality of wheat bread through faba bean sourdough addition. LWT-Food Sci Technol. 2017;82:296–302. https://doi.org/10.1016/j.lwt.2017.04.062
  67. 62. Nikmaram N, Leong SY, Koubaa M, Zhu Z, Barba FJ, Greiner R, et al. Effect of extrusion on the anti-nutritional factors of food products: An overview. Food control. 2017;79:62–73. https://doi.org/10.1016/j.foodcont.2017.03.027
  68. 63. Enneking D, Wink M, editors. Towards the elimination of anti-nutritional factors in grain legumes. Linking research and marketing opportunities for pulses in the 21st century: Proceedings of the third international food legumes research conference; 2000: Springer. https://doi.org/10.1007/978-94-011-4385-1_65
  69. 64. Wang L-J, Li D, Tatsumi E, Liu Z-S, Chen XD, Li L-T. Application of two-stage ohmic heating to tofu processing. Chem Eng Process: Process Intensif. 2007;46(5):486–90. https://doi.org/10.1016/j.cep.2006.06.017
  70. 65. Vearasilp T, Laenoi W, Vearasilp S, Krittigamas N, Lücke W, Pawelzik E, et al. Effect of radio frequency technique on nutrient quality and destruction of trypsin inhibitor in soybean. The Global Food and Product Chain-Dynamics, Innovations, Conflicts, Strategies Book of Abstracts. 2005;384.
  71. 66. Al-Kaisey MT, Alwan A-KH, Mohammad MH, Saeed AH. Effect of gamma irradiation on antinutritional factors in broad bean. Radiat Phys Chem. 2003;67(3-4):493–96. https://doi.org/10.1016/S0969-806X(03)00091-4
  72. 67. Tresina P, Sornalakshmi V, Mohan V. Impact of gamma irradiation on the nutritional and antinutritional qualities of Mucuna deeringiana (Bort) Merril: an underexploited food legume. Int J recent res asp. 2018;3(4):487–43.
  73. 68. Kayitesi E, Duodu KG, Minnaar A, de Kock HL. Effect of micronisation of pre‐conditioned cowpeas on cooking time and sensory properties of cooked cowpeas. J Sci Food Agric. 2013;93(4):838–45. https://doi.org/10.1002/jsfa.5805
  74. 69. Khokhar S, Apenten RKO. Antinutritional factors in food legumes and effects of processing. The role of food, agriculture, forestry and fisheries in human nutrition. 2003;4:82–116.
  75. 70. Ogundele OM, Gbashi S, Oyeyinka SA, Kayitesi E, Adebo OA. Optimization of infrared heating conditions for precooked cowpea production using response surface methodology. Molecules. 2021;26(20):6137. https://doi.org/10.3390/molecules26206137
  76. 71. George M, Bhide S, Thengane R, Hosseini G, Manjaya J. Identification of low lectin mutants in soybean. Plant breeding. 2008;127(2):150–53. https://doi.org/10.1111/j.1439-0523.2007.01449.x
  77. 72. Pramitha JL, Rana S, Aggarwal PR, Ravikesavan R, Joel AJ, Muthamilarasan M. Diverse role of phytic acid in plants and approaches to develop low-phytate grains to enhance bioavailability of micronutrients. Adv Genet. 2021;107:89–
  78. 120. https://doi.org/10.1016/bs.adgen.2020.11.003
  79. 73. Khazaei H, Purves RW, Hughes J, Link W, O'Sullivan DM, Schulman AH, et al. Eliminating vicine and convicine, the main anti-nutritional factors restricting faba bean usage. Trends Food Sci Technol. 2019;91:549–56. https://doi.org/10.1016/j.tifs.2019.07.051
  80. 74. Valentine MF, De Tar JR, Mookkan M, Firman JD, Zhang ZJ. Silencing of soybean raffinose synthase gene reduced raffinose family oligosaccharides and increased true metabolizable energy of poultry feed. Front Plant Sci. 2017;8:692. https://doi.org/10.3389/fpls.2017.00692
  81. 75. Das G, Sharma A, Sarkar PK. Conventional and emerging processing techniques for the post-harvest reduction of antinutrients in edible legumes. Appl Food Res. 2022;2(1):100112. https://doi.org/10.1016/j.afres.2022.100112

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