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Plant Science Today (PST)

Research Articles

Vol. 12 No. 2 (2025)

Polarity-driven extraction revealed potent bioactivities in rhizomes and leaves of Curcuma caesia Roxb.

DOI
https://doi.org/10.14719/pst.5832
Submitted
16 October 2024
Published
24-03-2025 — Updated on 01-04-2025
Versions

Abstract

The increasing demand for plant-based bioactive compounds has fueled interest in exploring natural antioxidants and antimicrobial agents. Curcuma caesia Roxb., commonly known as black turmeric, holds significant potential as a source of natural antioxidants and antimicrobial agents. This study investigated the antioxidant and antimicrobial potential of sequential extracts from C. caesia rhizomes and leaves, utilizing solvents of varying polarity (n-hexane, chloroform, ethyl acetate, methanol and water). The extraction yields varied between 1.69% and 6.34%, with n-hexane providing the highest yield of 6.34% for leaf extracts and 5.9% for rhizome extracts. Chloroform extracts were particularly rich in phenolics (total phenolic content: 95.17 ± 0.15 mg GAE/g for leaves and 84.16 ± 0.20 mg GAE/g for rhizome) and flavonoids (total flavonoids content: up to 75.98 ± 2.00 mg quercetin/g for leaves and 56.89 ± 0.15 mg quercetin/g for rhizomes). Antioxidant activity, determined through 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, showed the strongest results in chloroform extracts, with IC50 values as low as 0.75 ± 0.02 microgram/mL for leaves. Additionally, nonpolar solvent extracts (n-hexane and chloroform) demonstrated significant antimicrobial activity against multidrug-resistant strains like Klebsiella pneumoniae and Staphylococcus aureus, with minimum inhibitory concentration (MIC) values as low as 3.12 microgram/mL, comparable to standard antibiotics. These findings highlight C. caesia as a promising source of bioactive compounds for future phytopharmaceutical applications.

References

  1. Mac-Kalunta OM, Ahamefula AA, Odii C, Ibe B. Comparative studies of the yield and chemical constituents of Bryophyllumpinnatum by cold merceration and sohxlet extraction methods. J Chem Soc Nigeria. 2022;47(4):807-17. https://doi.org/10.46602/jcsn.v47i4.788
  2. Warrier PP, Badole M. Gas chromatography-mass spectrometric study of phytoconstituents in Curcuma caesia Roxb. rhizomes extract by different extraction techniques. Indian J Pharm Sci. 2023;85(6):1773-80. https://doi.org/10.36468/pharmaceutical-sciences.1234
  3. Jain A, Parihar DK. In-vitro antidiabetic potential of endangered Curcuma caesia rhizome extracts and its characterization by FTIR and GC/HR-MS analysis. Int J Adv Sci Engin Technol. 2018;6(4):16–22.
  4. Borah A, Paw M, Gogoi R, Loying R, Sarma N, Munda S, et al. Chemical composition, antioxidant, anti-inflammatory, anti-microbial and in-vitro cytotoxic efficacy of essential oil of Curcuma caesia Roxb. leaves: An endangered medicinal plant of Northeast India. Ind Crops Prod. 2019;129:448-54. https://doi.org/10.1016/j.indcrop.2018.12.035
  5. Sahu B, Kenwat R, Chandrakar R. Medicinal value of Curcuma cassia Roxb: An overview UK. J Pharm Biol Sci. 2016;6:69-74. https://doi.org/10.20510/ukjpb/4/i6/134671
  6. Hayouni EA, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem. 2007;105(3):1126-34. https://doi.org/10.1016/j.foodchem.2007.02.010
  7. Wojcikowski K, Stevenson L, Leach D, Wohlmuth H, Gobe G. Antioxidant capacity of 55 medicinal herbs traditionally used to treat the urinary system: A comparison using a sequential three-solvent extraction process. J Altern Complement Med. 2007;13(1):103-10. https://doi.org/10.1089/acm.2006.6122
  8. Khuntia S, Sahoo BC, Lenka J, Kar B, Sahoo S. In-silico prediction and in vitro validation of antioxidant, antibacterial and antifungal potential of black turmeric (Curcuma caesia Roxb.) essential oils and its constituents. Ind Crops Prod. 2023; 203:117185. https://doi.org/10.1016/j.indcrop.2023.117185
  9. Khuntia S, Lenka J, Dash M, Sahoo BC, Kar B, Sahoo S. Bioactivity screening of thirty black turmeric (Curcuma caesia Roxb.) essential oils against free radicals and MDR isolates. Pharmacogn Mag. 2023;(3):615-25. https://doi.org/10.1177/09731296231174958
  10. Suja KP, Jayalekshmy A, Arumugham C. Free radical scavenging behavior of antioxidant compounds of sesame (Sesamum indicum L.) in DPPH system. J Agric Food Chem. 2004;52:912-15. https://doi.org/10.1021/jf0303621
  11. Temple NJ. Antioxidants and disease: More questions than answers. Nutr Res. 2000;20:449-59. https://doi.org/10.1016/S0271-5317(00)00138-X
  12. Chatti IB, Boubaker J, Skandrani I, Bhouri W, Ghedira K, Chekir Ghedira L. Antioxidant and antigenotoxic activities in Acacia salicina extracts and its protective role against DNA strand scission induced by hydroxyl radical. Food Chem Toxicol. 2011;49:1753-58. https://doi.org/10.1016/j.fct.2011.04.022
  13. Chaves N, Santiago A, Alías JC. Quantification of the antioxidant activity of plant extracts: Analysis of sensitivity and hierarchization based on the method used. Antioxidants. 2020;9(1):76. https://doi.org/10.3390/antiox9010076
  14. IndustryARC™. Polyphenols market – Forecast (2021 - 2026). [Internet]. Available online: https://www.industryarc.com/Report/17797/polyphenols-market.html
  15. Ray A, Jena S, Dash B, Kar B, Halder T, Chatterjee T, et al. Chemical diversity, antioxidant and antimicrobial activities of the essential oils from Indian populations of Hedychium coronarium Koen. Ind Crops Prod. 2018;112:353–62. https://doi.org/10.1016/j.indcrop.2017.12.033
  16. Pandey D, Gupta AK. Phytochemical assessment and synergistic bioefficacy of Curcuma caesia (Roxb.) from Bastar against multi-drug-resistant human pathogens. IJPSR. 2022;13(12):1000-10. https://doi.org/10.13040/IJPSR.0975-8232.13(12).1000-10
  17. Pandey D, Gupta AK. Antibacterial efficacy of Curcuma caesia from Bastar district of Chhattisgarh, India. Int J Pharm Sci Res. 2014;5(6):2294. https://doi.org/10.13040/IJPSR.0975-8232.5(6).2294-01
  18. Sahoo S, Parida R, Singh S, Padhy RN, Nayak S. Evaluation of yield, quality and antioxidant activity of essential oil of in vitro propagated Kaempferia galanga Linn. J Acute Dis. 2014;3(2):124–30. https://doi.org/10.1016/S2221-6189(14)60028-7
  19. Dash M, Singh S, Sahoo BC, Sahoo S, Sahoo RK, Nayak S, et al. Potential role of Indian long pepper (Piper longum L.) volatiles against free radicals and multidrug-resistant isolates. Nat Prod Res. 2021;36(16):4271–75. https://doi.org/10.1080/14786419.2021.1975703
  20. Sahoo S, Ghosh G, Das D, Nayak S. Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra BL Burtt. Asian Pac J Trop Biomed. 2013;3(11):871-76. https://doi.org/10.1016/S2221-1691(13)60171-9
  21. Swamy MK, Sinniah UR, Akhtar MS. In vitro pharmacological activities and GC-MS analysis of different solvent extracts of Lantana camara leaves collected from tropical region of Malaysia. eCAM. 2015;(1):506413. https://doi.org/10.1155/2015/506413
  22. Kirmani F, Saddiqe Z, Saleem S, Ali F, Haq FU. Phytochemical investigation and antibacterial activity of Curcuma longa against multi-drug resistant bacteria. S Afr J Bot. 2024;164:137-45. https://doi.org/10.1016/j.sajb.2023.11.042
  23. Kamarudin NA, Markom M, Latip J. Effects of solvents and extraction methods on herbal plants Phyllanthus niruri, Orthosiphon stamineus and Labisia pumila. J Sci Technol. 2016;9(21):1-5. https://doi.org/10.17485/ijst/2016/v9i21/95235
  24. Daoud A, Malika D, Bakari S, Hfaiedh N, Mnafgui K, Kadri A, et al. Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of date palm pollen (DPP) from two Tunisian cultivars. Arab J Chem. 2019;12(8):3075-86. https://doi.org/10.1016/j.arabjc.2015.07.014
  25. Jeyaseelan EC, Jenothiny S, Pathmanathan MK, Jeyadevan JP. Antibacterial activity of sequentially extracted organic solvent extracts of fruits, flowers and leaves of Lawsonia inermis L. from Jaffna. Asian Pac J Trop Biomed. 2012;2(10):798-802. https://doi.org/10.1016/S2221-1691(12)60232-9

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