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Plant Science Today (PST)

Research Articles

Vol. 12 No. 2 (2025)

Preharvest melatonin application on enhancing quality and extending shelf life in papaya

DOI
https://doi.org/10.14719/pst.6192
Submitted
20 November 2024
Published
22-03-2025 — Updated on 01-04-2025
Versions

Abstract

Papaya is an important fruit crop, and its cultivation has gaining importance in recent times due to its high economic returns . However,due to its climacteric behaviour and high perishablility, the shelf life of papaya after harvest is very limited. A field experiment was conducted to evaluate the effect of preharvest melatonin applications at  different concentrations – 0.5 mM, 1.0 mM, 1.5 mM, and 2.0 mM, along with a control treatment (water spray) on enhancing  postharvest quality and prolonging  the shelf life of papaya. Among these, the applications of melatonin at 1.5 mM, administed 15 days before harvest, was found to be the most effective in extending the shelf life of papaya by significantly delaying changes in quality parameters. This  treatment also resulted in increased antioxidant enzymes activity, improved fruit firmness, delayed ripening enzyme activity, and  reduced weight loss. Papaya fruits harvested from trees sprayed with 1.5 mM melatonin exhibited a firmness of 6.37 kg/cm2 under ambient storage and and 6.26 kg/cm2 under cold storage,  compared to 5.54 kg/cm2 and 5.33 kg/cm2, respectively, for control fruits.  Additionally, total soluble solids (TSS) levels in fruits from the 1.5 mM melatonin treatment were recorded as 12.90 ºB  under ambient storage and 13.50 ºB under cold storage, compared to 15.00 ºB and 15.40 ºB, respectively, for control fruits. In conclusion, preharvest melatonin  application at 1.5mM effectively delayed postharvest senescence, enhanced fruit quality, and reduced postharvest losses in papaya. This approach could significantly improve market access and industrial adaptability, benefiting both producers and consumers.

References

  1. Papaya production worldwide. Statista. [Internet]. 2022. [ updated on 6 Feb 2024; cited on 4 Sept 2024]. https://www.statista.com/statistics/578039/world-papaya-production
  2. Area and production of horticulture crops. Indiastat [Internet]. 2023-24. [cited updated on 12 Jan 2025; cited on 17 Jan 2025]. https://www.indiastat.com/table/agriculture/area-production-productivity-papaya-india.
  3. Prasad K, Paul JR. Postharvest losses of papaya and practice for management. Food Sci Rep. 2021;2(7):1–15.
  4. Wu Q, Li Z, Chen X, Yun Z, Li T, Jiang Y. Comparative metabolites profiling of harvested papaya (Carica papaya L.) peel in response to chilling stress. J Sci Food Agri. 2019;99(15):6868–81. https://doi.org/10.1002/jsfa.9972
  5. Wang D, Randhawa MS, Azam M, Liu H, Ejaz S, Ilahy R, et al. Exogenous melatonin treatment reduces postharvest senescence and maintains the quality of papaya fruit during cold storage. Front Plant Sci. 2022;13:1039373. https://doi.org/10.3389/fpls.2022.1039373
  6. Xu T, Chen Y, Kang H. Melatonin is a potential target for improving post-harvest preservation of fruits and vegetables. Front Plant Sci. 2019;10:1388. https://doi.org/10.3389/fpls.2019.01388
  7. Liu S, Huang H, Huber DJ, Pan Y, Shi X, Zhang Z. Delay of ripening and softening in ‘Guifei’mango fruit by postharvest application of melatonin. Posthar Bio Techno.
  8. ;163:111136. https://doi.org/10.1016/j.postharvbio.2020.111136
  9. Aghdam MS, Fard JR. Melatonin treatment attenuates postharvest decay and maintains nutritional quality of strawberry fruits (Fragaria× anannasa cv. Selva) by enhancing GABA shunt activity. Food Chemistry. 2017;221:1650–57. https://doi.org/10.1016/j.foodchem.2016.10.123
  10. Hu W, Yang H, Tie W, Yan Y, Ding Z, Liu Y, et al. Natural variation in banana varieties highlights the role of melatonin in postharvest ripening and quality. J Agri Food Chem. 2017;65(46):9987–94. https://doi.org/10.1021/acs.jafc.7b03354
  11. Wang F, Zhang X, Yang Q, Zhao Q. Exogenous melatonin delays postharvest fruit senescence and maintains the quality of sweet cherries. Food Chemistry. 2019;301:125311. https://doi.org/10.1016/j.foodchem.2019.125311
  12. Sharafi Y, Jannatizadeh A, Fard JR, Aghdam MS. Melatonin treatment delays senescence and improves antioxidant potential of sweet cherry fruits during cold storage. Sci Horti. 2021;288:110304. https://doi.org/10.1016/j.scienta.2021.110304
  13. Wang Z, Pu H, Shan S, Zhang P, Li J, Song H, et al. Melatonin enhanced chilling tolerance and alleviated peel browning of banana fruit under low temperature storage. Posthar Bio Techno. 2021;179:111571. https://doi.org/10.1016/j.postharvbio.2021.111571
  14. Ranganna S. "Manual of analysis of fruit and vegetable products". Tata McGraw Hill Co. Ltd., New Delhi; 1977.
  15. Hedge J, Hofreiter B, Whistler R. "Carbohydrate chemistry." Academic Press, New York. 1962;17:371–80.
  16. Malik CP, Singh M. "Plant enzymology and histo-enzymology". Kalyani Publishers; 1980.
  17. Sadasivam S, Balasubramanian T. Practical manual in biochemistry. Tamil Nadu Agricultural University, Coimbatore, India. 1987;14.
  18. Srivastava MK, Dwivedi UN. Delayed ripening of banana fruit by salicylic acid. Plant Sci. 2000;158(1-2):87–96. https://doi.org/10.1016/S0168-9452(00)00304-6
  19. Hagerman AE, Austin PJ. Continuous spectrophotometric assay for plant pectin methyl esterase. J Agri Food Chem. 1986;34(3):440–44. https://doi.org/10.1021/jf00069a015
  20. Gayathri T, Nair AS. Biochemical analysis and activity profiling of fruit ripening enzymes in banana cultivars from Kerala. J Food Measure Character. 2017;11:1274–83. https://doi.org/10.1007/s11694-017-9505-6
  21. Li R, Wang Y, Li W, Shao Y. Comparative analyses of ripening, texture properties and cell wall composition in three tropical fruits treated with 1-methylcyclopropene during cold storage.
  22. Horticulturae. 2023;9(2):126. https://doi.org/10.3390/horticulturae9020126
  23. Pütter J. Peroxidases. Methods of enzymatic analysis. Elsevier; 1974. p. 685–90. https://doi.org/10.1016/B978-0-12-091302-2.50033-5
  24. Aebi H. Catalase in vitro. Methods in enzymology. 105: Elsevier; 1984. p. 121–26. https://doi.org/10.1016/S0076-6879(84)05016-3
  25. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analy Biochem. 1971;44(1):276–87. https://doi.org/10.1016/0003-2697(71)90370-8
  26. Fabi JP, Cordenunsi BR, de Mattos Barreto GP, Mercadante AZ, Lajolo FM, Oliveira do Nascimento JR. Papaya fruit ripening: response to ethylene and 1-methylcyclopropene (1-MCP). J Agri food Chem. 2007;55(15):6118–23. https://doi.org/ 10.1021/jf070903c
  27. Vitalini S, Gardana C, Zanzotto A, Simonetti P, Faoro F, Fico G, et al. The presence of melatonin in grapevine (Vitis vinifera L.) berry tissues. J Pineal Res. 2011;51(3):331–37. https://doi.org/10.1111/j.1600-079X.2011.00893.x
  28. Meng JF, Xu TF, Song CZ, Yu Y, Hu F, Zhang L, et al. Melatonin treatment of pre-veraison grape berries to increase size and synchronicity of berries and modify wine aroma components. Food Chem. 2015;185:127–34. https://doi.org/10.1016/j.foodchem.2015.03.140
  29. Zhao Y, Tan DX, Lei Q, Chen H, Wang L, Li Qt, et al. Melatonin and its potential biological functions in the fruits of sweet cherry. J Pineal Res. 2013;55(1):79–88. https://doi.org/10.1111/jpi.12044
  30. El-Naby A, Abdelkhalek AMM, El-Naggar YIM. Effect of melatonin, GA3 and NAA on vegetative growth, yield and quality of ‘Canino’apricot fruits. Acta Sci Polo Hort Cultus. 2019;18(3). https://doi.org/10.1007/s11947-012-1013-4
  31. Ong MK, Kazi FK, Forney CF, Ali A. Effect of gaseous ozone on papaya anthracnose. Food Biopro Techno. 2013;6:2996–3005. https://doi.org/10.1007/s11947-012-1013-4
  32. Liu C, Zheng H, Sheng K, Liu W, Zheng L. Effects of melatonin treatment on the postharvest quality of strawberry fruit. Posthar Bio Techno. 2018;139:47–55. https://doi.org/10.1016/j.postharvbio.2018.01.016
  33. Gao H, Zhang ZK, Chai HK, Cheng N, Yang Y, Wang DN, et al. Melatonin treatment delays postharvest senescence and regulates reactive oxygen species metabolism in peach fruit. Posthar Bio Techno. 2016;118:103–10. https://doi.org/10.1016/j.postharvbio.2016.03.006
  34. Zhai R, Liu J, Liu F, Zhao Y, Liu L, Fang C, et al. Melatonin limited ethylene production, softening and reduced physiology disorder in pear (Pyrus communis L.) fruit during senescence. Posthar Bio Techno. 2018;139:38–46. https://doi.org/10.1016/j.postharvbio.2018.01.017
  35. Kucuker E, Aglar E, Sakalda? M, ?en F, Gundogdu M. Impact of postharvest putrescine treatments on phenolic compounds, antioxidant capacity, organic acid contents and some quality characteristics of fresh fig fruits during cold storage. Plants. 2023;12(6):1291. https://doi.org/10.3390/plants12061291
  36. Anchana K, Kavitha C, Shanmugasundaram K, Djanaguiraman M, Johnson I. Role of exogenous melatonin in enhancing shelf life of traditional banana varieties. Int J Environ Climate Change. 2023;13(10):992–98. https://doi.org/10.9734/ijecc/2023/v13i102746
  37. Fan S, Xiong T, Lei Q, Tan Q, Cai J, Song Z, et al. Melatonin treatment improves postharvest preservation and resistance of guava fruit (Psidium guajava L.). Foods. 2022;11(3):262. https://doi.org/10.3390/foods11030262
  38. Bal E. Effect of melatonin treatments on biochemical quality and postharvest life of nectarines. J Food Measure Character. 2021;15(1):288–95. https://doi.org/10.1007/s11694-020-00636-5
  39. Etienne A, Génard M, Lobit P, Mbeguié-A-Mbéguié D, Bugaud C. What controls fleshy fruit acidity? A review of malate and citrate accumulation in fruit cells. J Experi Botany. 2013;64(6):1451–69. https://doi.org/10.1093/jxb/ert035
  40. Hazarika T, Marak T. Salicylic acid and oxalic acid in enhancing the quality and extending the shelf life of grape cv. Thompson seedless. Food Sci Techno Int. 2022;28(6):463–75. https://doi.org/10.1177/10820132211020612
  41. Hanif A, Ahmad S, Shahzad S, Liaquat M, Anwar R. Postharvest application of salicylic acid reduced decay and enhanced storage life of papaya fruit during cold storage. J Food Measure Character. 2020;14:3078–88. https://doi.org/10.1007/s11694-020-00555-5
  42. Aghdam MS, Luo Z, Jannatizadeh A, Sheikh-Assadi M, Sharafi Y, Farmani B, et al. Employing exogenous melatonin applying confers chilling tolerance in tomato fruits by upregulating ZAT2/6/12 giving rise to promoting endogenous polyamines, proline and nitric oxide accumulation by triggering arginine pathway activity. Food Chem. 2019;275:549–56. https://doi.org/10.1016/j.foodchem.2018.09.157
  43. Wang L, Luo Z, Ban Z, Jiang N, Yang M, Li L. Role of exogenous melatonin involved in phenolic metabolism of Zizyphus jujuba fruit. Food Chem. 2021;341:128268. https://doi.org/10.1016/j.foodchem.2020.128268
  44. Gest N, Gautier H, Stevens R. Ascorbate as seen through plant evolution: the rise of a successful molecule?. J Experi Botany. 2013;64(1):33–53. https://doi.org/10.1093/jxb/ers297
  45. Zheng X, Gong M, Zhang Q, Tan H, Li L, Tang Y, et al. Metabolism and regulation of ascorbic acid in fruits. Plants. 2022;11(12):1602. https://doi.org/10.3390/plants11121602
  46. Yahia EM, Carrillo-Lopez A. Postharvest physiology and biochemistry of fruits and vegetables. Woodhead publishing; 2018.
  47. Nasser MA, El-Mogy MM, Samaan MS, Hassan KM, El-Sayed SM, Alsubeie MS, et al. Postharvest exogenous melatonin treatment of table grape berry enhances quality and maintains bioactive compounds during refrigerated storage. Horticulturae. 2022;8(10):860. https://doi.org/10.3390/horticulturae8100860
  48. Liu B, Xin Q, Zhang M, Chen J, Lu Q, Zhou X, et al. Research progress on mango post-harvest ripening physiology and the regulatory technologies. Foods. 2022;12(1):173. https://doi.org/10.3390/foods12010173
  49. Xia Y, Wu DT, Ali M, Liu Y, Zhuang QG, Wadood SA, et al. Innovative postharvest strategies for maintaining the quality of kiwifruit during storage: An updated review. Food Front. 2024;5:1933–50. https://doi.org/10.1002/fft2.442
  50. Njie A, Zhang We, Dong X, Lu C, Pan X, Liu Q. Effect of melatonin on fruit quality via decay inhibition and enhancement of antioxidative enzyme activities and genes expression of two mango cultivars during cold storage. Foods. 2022;11(20):3209. https://doi.org/10.3390/foods11203209
  51. Madebo MP, Hu S, Zheng Y, Jin P. Mechanisms of chilling tolerance in melatonin treated postharvest fruits and vegetables: A review. J Future Foods. 2021;1(2):156–67. https://doi.org/10.3389/fpls.2019.01388

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