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Early Access

Influence of ethrel on spatiotemporal changes and biosynthesis of volatile metabolites of Mango cultivars

DOI
https://doi.org/10.14719/pst.6641
Submitted
10 December 2024
Published
27-02-2025
Versions

Abstract

Mango, a highly preferred fruit known for its distinctive aroma and flavour, requires proper ripening to enhance its quality and shelf life. The use of calcium carbide for ripening has been banned due to its harmful effects on human health. While ethrel treatment in temperature-controlled chambers offers a safer alternative, it remains unaffordable for small-scale farmers and traders. This study evaluated mango ripening using ethrel in three chambers: silpaulin chamber, zero energy cool chambers, and cold chamber. Among these, the zero-energy cool chambers demonstrated the most promising results. Fruits ripened in this chamber exhibited the lowest per cent disease index, indicating superior quality. Additionally, these fruits recorded higher colour values and total carotenoid content than those ripened in the cold chamber. The silpaulin chamber, however, showed increased antioxidant enzyme activity due to a higher respiration rate and disease index. Importantly, mangoes ripened in the zero energy cool chambers had the highest area percentage of volatile metabolites, which are key contributors to aroma and defence mechanisms. This suggests that the zero-energy cool chamber enhances fruit quality while minimizing postharvest losses. Besides being an eco-friendly and cost-effective alternative to traditional cold chambers, the zero-energy cool chamber can serve dual purposes. When not used for ripening, it can be utilized for the postharvest storage of fruits, effectively extending their shelf life. Thus, adopting zero-energy cool chambers offers a sustainable solution for small-scale mango traders and farmers.

References

  1. 1. NHB. Area and production of horticulture crops. 2nd Advance Estimate as per PIB Data base [internet]. Gurgaon: NHB; 2022[cited 24 Sept 2024]. Available from:www.nhb.gov.in.
  2. 2. Hossain M, Akhtar S, Anwar M. Health hazards posed by the consumption of artificially ripened fruits in Bangladesh. Int Food Res J. 2015;22(5):1755–60.
  3. 3. Mursalat M, Rony AH, Rahman AHMS, Islam MN, Khan MS. A critical analysis of artificial fruit ripening: Scientific, legislative and socio-economic aspects. Chem Engineer Sci Mag. 2013;4(1):1–7.
  4. 4. Kaur, Sukhjit. Effect of different treatments of ethrel on ripening behaviour and post- harvest quality of mango (Mangifera indica L.) during storage. J Appl Nat Sci. 2017;9:85–93. https://doi.org/10.31018/jans.v9i1.1155
  5. 5. Meena BL, Khan SA, Srivastava V. Current scenario of gi certified mango varieties in India. Economic Aff. 2022;67(4): 681-90 https://doi.org/10.46852/0424-2513.4.2022.34
  6. 6. Maske J, Masih S,Verma O. A review on morphological and molecular characterization of Colletotrichum species associated with mango anthracnose in Konkan region of Maharashtra state. The Pharma Innov J. 2022;11(5):1577–81.
  7. 7. Ismail NS, Rasdi I, PravemaSM, Abidni EZ. Knowledge, attitude and practice associated with calcium carbide used for fruit ripening among mango farmers, farm workers and fruit traders. Mal J Med and Health Sci. 2018;14(2):11–17.
  8. 8. Eze EE, Okpako JEF. Practices towards artificial fruit ripening among fruit vendors in rivers state. Int J Rec Innov Acad Res. 2021;5(8):85–93. https://doi.org/10.14738/assrj.87.10589
  9. 9. Nunes MCN, Emond J, Brecht JK, Dea S, Proulx E. Quality curves for mango fruit (cv. Tommy Atkins and Palmer) stored at chilling and nonchilling temperatures. J Food Qual. 2007;30(1):104–20. https://doi.org/10.1111/j.1745-4557.2007.00109.x
  10. 10. Narayana C, Pal R, Roy S. Effect of studies on ripening changes in mango pre-storage treatments and temperature regimes on shelf-life and respiratory behaviour of ripe Baneshan mango. J Food Sci Techno. 1996;33:79–82.
  11. 11. Waskar DP. Hot water treatment for disease control and extension of shelf life of 'Kesar' mango (Mangifera indica L.) fruits. Acta Hortic. 2005;682.1319–24. 10.17660/ActaHortic.2005.682.177.
  12. 12. Mohamed HE, AbuGoukh AA. Effect of ethrel in aqueous solution and ethylene released from ethrel on mango fruit ripening. J Horti Sci and Biotech. 2003;78(4):568–73.https://doiorg101080/1620316.2003.11511665.
  13. 13. Kader AA. US grade standards. Postharvest Tech Hortic Crops. 2002;3311(287):287–300.
  14. 14. Akula S, Paidighanta PR, Dubasi GR. Influene of source and quality on the colour characteristics of annatto dyes and formulations. LWT- Food Sci Techno. 2010;43(9):1456–60. https://doi.org/10.1016/j.lwt.2010.04.022
  15. 15. Roy SK. Simple and rapid method for estimation of total carotenoid pigments in mango. J Food Sci Techno. 1973;10:38–42.
  16. 16. Lakshmi B, Reddy P, Prasad R.Cross-infection potential of Colletotrichum gloeosporioides Penz. isolates causing anthracnose in subtropical fruit crops. Trop Agric Res. 2011;22(2): https://doi.org/10.4038/tar.v22i2.2827
  17. 17. Aebi H. Catalase in vitro. Methods in Enzymo. 1984;105(C). https://doi.org/10.1016/S0076-6879(84)05016-3
  18. 18. Malik M, Singh DV. Analysis of finite magneto hydrodynamic. J Bear Wear. 1980;64(2):273–80. https://doi.org/10.1016/0043-1648(80)90133-7
  19. 19. Ibrahim A, Sani A, Manga S, Aliero A, Joseph R, Yakubu S, Ibafidon H. Microorganisms associated with volatile metabolites production in soft rot disease of sweet pepper fruits (Tattase). Int J Biotechn Biochem. 2011;7(2):217–28.
  20. 20. Yunchalad M, Yves L, Claudie D. Comparison of aroma components in Thai mango (cv. Kaew) from different extraction methods. Proceed ASEAN Food Conf Singapore; 1997. 24–27.
  21. 21. Nanthachai N, LichanpornI I, TanganuratP, Singkum U. Efficiency of crude extract from pummelo peel on controlling the growth of Colletotrichum gloeosporioides (Penz.). Int J Environ Rural Develop. 2015;6(2):17–22. https://doi.org/10.32115/ijerd.6.2_17
  22. 22. Nambi VE, Thangavel K, Jesudas DM. Scientific classification of ripening period and development of colour grade chart for Indian mangoes (Mangifera indica L.) using multivariate cluster analysis. Sci Hortic. 2015;193:90–98. https://doi.org/10.1016/j.scienta.2015.05.031
  23. 23. Gill P, Jawandha S, Kaur N. Transitions in mesocarp colour of mango fruits kept under variable temperatures. J Food Sci Techno. 2017;54:4251–56.https://doi.org/10.1007/s13197-017-2894-z
  24. 24. Villalobos MdC, Serradilla MJ, Martín A, Corrales LM, Pereira C, Córdoba MdG. Preservation of different fig cultivars (Ficus carica L.) under modified atmosphere packaging during cold storage. J Sci Food Agric. 2016;96(6):2103‒15.https://doi.org/10.1002/jsfa.7326
  25. 25. Chen M, Gu H, Wang L, Shao Y, Li R, Li W. Exogenous ethylene promotes peel color transformation by regulating the degradation of chlorophyll and synthesis of anthocyanin in postharvest mango fruit. Fron Nutr. 2022;9:911542.https://doi.org/10.3389/fnut.2022.911542
  26. 26. Ornelas-Paz JDJ, YahiaE M, Gardea-Bejar A. Identification and quantification of xanthophyll esters, carotenes and tocopherols in the fruit of seven Mexican mango cultivars by liquid chromatography-atmospheric pressure chemical ionization-time-of-flight mass spectrometry [LC-(APcI+)-MS]. J Agric Food Chem. 2007;55(16):4527-531 https://doi.org/10.1021/jf0706981
  27. 27. Subramanyam H, Sebastian K. Effect of succinic acid 2, 2-dimethyl hydrazide on carotene development in 'Alphonso' mango. J Am Soc Hortic Sci. 2022;5(3): 160-61 https://doi.org/10.21273/jashs.5.3.160
  28. 28. Vijayanand P, Deepu E, Kulkarni S. Physico chemical characterization and the effect of processing on the quality characteristics of Sindura, Mallika and Totapuri mango cultivars. J Food Sci Techn. 2015;52:1047–53. https://doi.org/10.1007/s13197-013-1041-8
  29. 29. Veena G, Muralidhara B, Rajan S. Genetic diversity of mango (Mangifera indica) bioactive components. Indian J Agric Sci. 2019;89:2107–10.https://doi.org/10.56093/ijas.v89i12.96283
  30. 30. Lalel J, Singh Z, Tan S. Ripening temperatures influence biosynthesis of aroma volatile compounds in Kensington Pride' mango fruit. J Hortic Sci Biotech. 2004;79(1):146–57.https://doi.org/10.1080/14620316.2004.11511729
  31. 31. Thomas P, Janave MT. Effects of gamma irradiation and storage temperature on carotenoids and ascorbic acid content of mangoes on ripening. J Sci Food and Agri. 1975;26(10):1503–12.https://doi.org/10.1002/jsfa.2740261009
  32. 32. Malik MT, Tariq T, Khan AH, Ullah H, Imran M, Iqbal J, Zainab A. Outbreak of anthracnose and stem end rot diseases of mango in changing climate and their management through hot water treatment. Pak J Phytopatho. 2018;30(1):91–98. https://doi.org/10.33866/phytopathol.030.01.0449
  33. 33. Nahar K, Naznin H, Hossain M. Susceptibility of mango to stem-end rot and anthracnose and its control through chemical and hot water treatment. J Agrofores Environ. 2017;1(2):1‒5.
  34. 34. Waskar D, Gaikwad R. Postharvest hot water treatment for disease control in kesar mango fruits. Ind J Agric Res. 2005;39(3):186–91.
  35. 35. Zhang J, Swingle PP. Effects of curing on green mold and stem-end rot of citrus fruit and its potential application under Florida packing system. Plant Dis. 2005;89(8):834–40. https://doi.org/10.1094/PD-89-0834
  36. 36. Zhang J. Lasiodiplodia theobromae in citrus fruit (Diplodia stem-end rot). In: Baños SB, editors. Postharvest decay. Academic Press; 2014. 309–35. https://doi.org/10.1016/B978-0-12-411552-1.00010-7
  37. 37. Ahmad S, Thompson AK, Hafiz A, Asi AA. Effect of temperature on the ripening behavior and quality of banana fruit. Int J Agric Biol. 2001;3(2):224–27.
  38. 38. Kankam F, Larbi-Koranteng S, Adomako J, Kwodaga JK, Akpatsu IB, Danso Y, Sowley ENK. Anthracnose disease of mango: epidemiology, impact and management options. In Cristiano Bellé C, editor. Current and emerging challenges in the diseases of trees. IntechOpen; 2022. https://doi.org/10.5772/intechopen.105934
  39. 39. Rao DVR, Chundawat BS. Post harvest changes in respiration and enzyme activities in sapota (Manilkara achras (Mill.) Forsberg). Ind J Pl Physio. 1989:32(2)105–09.
  40. 40. Pal DK, Selvaraj Y. Biochemistry of papaya (Carica papaya L.) fruit ripening: changes in RNA, DNA, protein and enzymes of mitochondrial, carbohydrate, respiratory and phosphate metabolism. J Hortic Sci. 1987; 62(1):117–24. https://doi.org/10.1080/14620316.1987.11515759
  41. 41. Singh R, Dwivedi UN. Effect of ethrel and 1-methylcyclopropene (1-MCP) on antioxidantsin mango (Mangifera indica var. Dashehari) during fruit ripening. Food Chem. 2008;111(4):951–56. https://doi.org/10.1016/j.foodchem.2008.05.011
  42. 42. Venkatesan T, Tamilmani C. Effect of ethrel on phenolic changes during ripening of off-season fruits of mango (Mangifera indica L. var. Neelum). Curr Bot. 2010;1(1):22–28.
  43. 43. Saunders AM. Histochemical identification of acid mucopolysaccharides with acridine orange. J Histochem Cytochem. 1964;12(3):164–70. https://doi.org/10.1177/12.3.164
  44. 44. Mattoo AK, Modi VV. Ethylene and ripening of mangoes. Plant Physio. 1969;44(2):308. https://doi.org/10.1104/pp.44.2.308
  45. 45. Trejo-Márquez MA, Ramírez-Villatoro G, Rosa CNA. Polyphenol oxidase and peroxidase activities in mangoes stored at chilling temperature. Acta Hortic. 2004;864:395–402. https://doi.org/10.17660/actahortic.2010.864.54
  46. 46. Rao MV, Paliyath G, Ormrod DP. Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physio. 1996;110(1):125–36. https://doi.org/10.1104/pp.110.1.125
  47. 47. Pino JA, Mesa J, Muñoz Y, Martí MP, Marbot R. Volatile components from mango (Mangifera indica L.) cultivars. J Agric Food Chem. 2005;53(6): 2213-223 https://doi.org/10.1021/jf0402633
  48. 48. Quijano CE, Salamanca G, Pino JA. Aroma volatile constituents of Colombian varieties of mango (Mangifera indica L.). Flav Fragr J. 2007;22(5):401–06. https://doi.org/10.1002/ffj.1812
  49. 49. Chidley HG, Kulkarni RS, Pujari KH, Giri AP, Gupta VS. Spatial and temporal changes in the volatile profile of Alphonso mango upon exogenous ethylene treatment. Food Chem. 2013;136(2):585–94. https://doi.org/10.1016/j.foodchem.2012.08.029
  50. 50. Idsteom H, Schreier P. Volatile constituents of Alphonso mango (Mangifera indica). Phytochem. 1985;24(10):2313–16. https://doi.org/10.1016/S0031-9422(00)83033-2
  51. 51. Jiang Y, Song J. Fruits and fruit flavor: Classification and biological characterization. In: Hui YH, editors. Handbook of fruit and vegetable flavors. New York: Wiley; 2010. pp. 1–23. https://doi.org/10.1002/9780470622834.ch1
  52. 52. Pandit SS, Kulkarni RS, Chidley HG, Giri AP, Pujari KH, Köllner TG, et al. Changes in volatile composition during fruit development and ripening of 'Alphonso' mango. J Sci Food Agric. 2009;89(12):2071–81. https://doi.org/10.1002/jsfa.3692
  53. 53. Pino JA, Mesa J. Contribution of volatile compounds to mango (Mangifera indica L.) aroma. Flav Fragran J. 2006;21(2):207–13. https://doi.org/10.1002/ffj.1703
  54. 54. Kulkarni RS, Chidley HG, Pujari KH, Giri AP, Gupta VS. Geographic variation in the flavour volatiles of Alphonso mango. Food Chem. 2012;130(1):58–66. https://doi.org/10.1016/j.foodchem.2011.06.053
  55. 55. Hunter G, Bucek WA, Radford T. Volatile components of canned Alphonso mango. J Food Sci. 1974;39(5):900–03. https://doi.org/10.1111/j.1365-2621.1974.tb07271.x
  56. 56. Engel KH, Tressl R. Studies on the volatile components of two mango varieties. J Agric Food Chem. 1983;31(4): 796-801 https://doi.org/10.1021/jf00118a029
  57. 57. Rapparini F, Predieri S. Pear fruit volatiles. Horticultural Rev. 2002;28:237–324. https://doi.org/10.1002/9780470650851.ch5
  58. 58. Qin L, Wei QP, Kang WH, Zhang Q, Sun J, Liu SZ. Comparison of volatile compounds in 'Fuji' apples in the different regions in China. Food Sci Techn Res. 2017;23(1):79–89. https://doi.org/10.3136/fstr.23.79
  59. 59. Walters D, Raynor L, Mitchell A, Walker R, Walker K. Antifungal activities of four fatty acids against plant pathogenic fungi. Mycopatho. 2004;157:87–90. https://doi.org/10.1023/B:myco.0000012222.68156.2C
  60. 60. Duan Y, Dong X, Liu B, Li P. Relationship of changes in the fatty acid compositions and fruit softening in peach (Prunus persica L. Batsch). Acta Physiol Plant. 2013;35:707–13. https://doi.org/10.1007/s11738-012-1111-y
  61. 61. Gholap AS, Bandyopadhyay C. Fatty acid biogenesis in ripening mango (Mangifera indica L. var. Alphonso). J Agric Food Chem. 1980;28(4): 839-41 https://doi.org/10.1021/jf60230a024
  62. 62. Wilson CW, Shaw PE, Knight RJ. Importance of some lactones and 2,5-Dimethyl-4-hydroxy-3(2H)- furanone to mango (Mangifera indica L.). Aroma J Agric Food Chem. 1990;38(7): https://doi.org/10.1021/jf00097a028
  63. 63. Bodoprost J, Rosemeyer H. Analysis of phenacylester derivatives of fatty acids from human skin surface sebum by reversed-phase HPLC: chromatographic mobility as a function of physico-chemical properties. Int J Mole Sci. 2007;8(11):1111–24. https://doi.org/10.3390/i8111111
  64. 64. Ansari MA, Asiri SM, Alzohairy MA, Alomary MN, Almatroudi A, Khan FA. Biofabricated fatty acids-capped silver nanoparticles as potential antibacterial, antifungal, antibiofilm and anticancer agents. Pharmaceut. 2021;14(2):139. https://doi.org/10.3390/ph14020139
  65. 65. Daniels A, Temikotan T, Ibiyemi D. Identification and charaterization of fatty acids, phytohemical properties and antibacterial effect of the ethyl acetate extract of Pilio stigmareticulatum. J Biotechnol Bioengin. 2021;5:30–40. https://doi.org/10.22259/2637-5362.0501005
  66. 66. Vendramini AL, Trugo LC. Chemical composition of acerola fruit (Malpighia punicifolia L.) at three stages of maturity. Food Chem. 2000;71(2):195–98. https://doi.org/10.1016/S0308-8146(00)00152-7
  67. 67. Mezroua EY, Agli A, Flamini G, Boudalia S, Oulamara H. Aroma characterization of ripe date fruits (Phoenix dactylifera L.) from Algeria. Afr J Biotechnol. 2017;16(42):2054–61. https://dx.doi.org/10.5897/ajb2017.16222
  68. 68. Jaleel W, Li Q, Shi Q, Qi G, Latif M, Ali S, et al. Using GCMS to find out the volatile components in the aroma of three different commercial fruits in China. J Anim Plant Sci. 2021;31(1):166‒74. https://doi.org/10.36899/japs.2021.1.0204
  69. 69. Valarmathi R, Natarajan D, Suryadevara NMNHM, Ragavan NDCAS, Vairavan CN. GC-MS analysis and antibacterial activity of Dryopteris hirtipes (Blumze) Kuntze Linn. J Survey Fisher Sci. 2023;10(1S):3718–26. https://doi.org/10.17762/sfs.v10i1S.815
  70. 70. Shahar B, Dolma N, Chongtham N. Phytochemical analysis, antioxidant activity and identification of bioactive constituents from three wild medicinally important underutilized plants of Ladak, India using GCMS and FTIR based metabolomics approach. Food Human. 2023;1:430–39. https://doi.org/10.1016/j.foohum.2023.06.022

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