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Plant Science Today (PST)

Research Articles

Early Access

Mushroom-mediated silver nanoparticle synthesis: characterisation, antimicrobial and antioxidant activities

DOI
https://doi.org/10.14719/pst.6744
Submitted
17 December 2024
Published
17-02-2025
Versions

Abstract

The use of biological agents for the synthesis of green nanoparticles has garnered significant attention, emerging as a promising approach in nanotechnology and materials science. In this study, silver nanoparticles were synthesised using an aqueous extract from cultivated mushrooms, including Chlorophyllum agaricoides PP410314, Coriolopsis trogii PP921338.1, Ganoderma sp. PP921328.1 and Lentinus tigrinus PP921339.1, grown on potato dextrose agar (PDA). The formation of these nanoparticles was confirmed through UV-visible spectroscopy, with maximum absorbance observed at 424 and 426 nm. Nanoparticles were characterised to assess their stability, shape, size and crystallinity using various analytical techniques, such as Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), scanning electron microscopy (SEM) and X-ray diffraction (XRD). The XRD pattern revealed 2? values corresponding to silver nanocrystals, with average crystallite sizes of 25.31, 27.05, 28.98 and 31.42 nm. The antimicrobial activity of the synthesised nanoparticles was tested against various microorganisms, including Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC9027, Staphylococcus aureus ATCC6538 and Candida albicans ATCC10231, demonstrating a strong inhibitory effect. Furthermore, antioxidant assays confirmed that these nanoparticles exhibited significant activity, which increased with concentration, in comparison to vitamin C. Overall, the green synthesis approach successfully produces silver nanoparticles with robust antioxidant and antibacterial properties, which can be attributed to the bioactive molecules present on their surface.

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