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Plant Science Today (PST)

Research Articles

Early Access

Biological suppression of Meloidogyne incognita in Coleus forskohlii using Pasteuria penetrans oil dispersion formulation

DOI
https://doi.org/10.14719/pst.7135
Submitted
8 January 2025
Published
03-03-2025
Versions

Abstract

Medicinal coleus (Coleus forskohlii) is an important herbal plant in Indian medicine. Root-knot nematode, Meloidogyne incognita, is the predominant pest of medicinal coleus and causes a yield reduction of up to 72%. This nematode affects the medicinal properties of tubers by producing root-knot galls. Chemical nematicides are unsuitable because the molecule forskolin, extracted from the tubers, could be contaminated. Hence, biological control is the preferred solution to prevent issues associated with pesticide residues. Pasteuria penetrans is a potential antagonistic bacterium of M. incognita. In this study, an oil dispersion formulation (ODF) of P. penetrans was developed and its effectiveness against M. incognita was tested. The formulation was water soluble and the spores were viable even after 60 days of incubation, as confirmed through juvenile parasitisation. Various concentrations of this formulation were prepared and screened against M. incognita infection in C. forskohlii under glasshouse conditions. Soil drenching with the formulation at a rate of 400 µL in 1000 mL of water resulted in an 86% reduction in egg mass production. P. penetrans endospores were recovered from treated plants. Based on these results, field trials were conducted over two seasons. The findings demonstrated that the application of ODF @ 4 L/ha in 200 L of water significantly reduced nematode infection, with 74.9%-84.8% and 61%- 66.5% reductions in egg mass production and gall index, respectively. Furthermore, the treatment enhanced tuber weight, with an increase of 32.9%-55.5% compared to carbofuran-treated plants.

References

  1. Jones JT, Haegeman A, Danchin EG, Gaur HS, Helder J, Jones MG, et al. Top 10 plant parasitic nematodes in molecular plant pathology. Mol Plant Pathol. 2013;14(9):946–61. https://doi.org/10.1111/mpp.12057
  2. Manjunatha T, Gowda AB, Rai, Singh B. Root knot nematode a threat to vegetable production and its management. IIVR Tech Bul. 2017;76.
  3. Singh R, Gangwar S, Singh D, Singh R, Pandey R, Kalra A. Medicinal plant Coleus forskohii Briq: Disease and management. Med Plants - Int J Phytomed Relat Ind. 2011;3(1):1–7. https://doi.org/10.5958/j.0975-4261.3.1.001
  4. Degenkolb T, Vilcinskas A. Metabolites from nematophagous fungi and nematicidal natural products from fungi as an alternative for biological control. Part I: metabolites from nematophagous ascomycetes. Appl Microbiol Biotechnol. 2016;100(9):3799–812. https://doi.org/10.1007/s00253-015-7233-6
  5. Stirling G. Biological control of plant parasitic nematodes. Wallingford. Oxon: CAB International. 1991;ISBNO-85198703-6.
  6. Chen Z, Dickson D, McSorley R, Mitchell D, Hewlett T. Suppression of Meloidogyne arenaria race 1 by soil application of endospores of Pasteuria penetrans. J Nematol. 1996;28(2):159.
  7. Hewlett TE, Gerber JF, Smith KS. In vitro culture of Pasteuria penetrans. Proceedings of the Fourth International Congress of Nematology, 8–13 June 2002, Tenerife, Spain; 2004. https://doi.org/10.1163/9789004475236_020
  8. Vagelas I, Leontopoulos S. Modeling the over dispersion of Pasteuria penetrans Endospores. Parasitologia. 2022;2(3):206–27. https://doi.org/10.3390/parasitologia2030018
  9. Senthamarai M, Poornima K, Subramanian S. Pathogenicity of Meloidogyne incognita on Coleus forskohlii Briq. Indian J Nematol. 2006;36(1):123–25.
  10. Bhandari S, Harsh NSK, Singh P. First report of Meloidogyne arenaria on Coleus forskohlii. Indian Forester. 2007;133:1709–10.
  11. Senthamarai K, Poornima K, Subramanian, Sudheer J. Nematode-fungal disease complex involving Meloidogyne incognita and Macrophomina phaseolina on medicinal Coleus, Coleus forskohlii Briq. Indian J Nematol. 2008;38(1):30–33.
  12. Zhou H, Yue Y, Liu G, Li Y, Zhang J, Gong Q, et al. Preparation and characterization of a lecithin nano OD as a topical delivery system. Nanoscale Res Lett. 2010;5(1):224–30. https://doi.org/10.1007/s11671-009-9469-5
  13. Cobb NA. Estimating the nematode population of the soil. United States Department of Agricultural Technology Circular, Washington. 1918;(1):48.
  14. Schindler AF. A simple substitute for Baermann funnel. Plant Dis Rep. 1961;45:747–48.
  15. McBeth CW, Taylor AL, Smith AL. Note on staining nematodes in root tissues. Proc Helminthol Soc Wash. 1941;8:26.
  16. Bridge J, Page SLJ. Estimation of root knot nematode infestation levels in roots using a rating chart. Trop Pest Manag. 1980;26:296–98. https://doi.org/10.1080/09670878009414416.
  17. Castagnone-Sereno P, Esparrago G, Abad P, Fredéric L, Michel B. Satellite DNA as a target for PCR-specific detection of the plant-parasitic nematode Meloidogyne hapla. Curr Genet. 1995;28:566–70. https://doi.org/10.1007/BF00518170
  18. Panse VG, Sukhatme PV. Statistical methods for agricultural workers. Indian Council of Agricultural Research, Publication, New Delhi; 1967
  19. Weibelzahl-Fulton E, Dickson D, Whitty E. Suppression of Meloidogyne incognita and M. javanica by Pasteuria penetrans in field soil. J Nematol. 1996;28(1):43–49.
  20. Mukhtar T, Arshad HM, Zameer KM. Biocontrol potential of Pasteuria penetrans, Pochonia chlamydosporia, Paecilomyces lilacinus and Trichoderma harzianum against Meloidogyne incognita in okra. Phytopathol Mediterr. 2013;52(1):66–76.
  21. Kamran M, Javed N, Khan SA, Jaskani MJ, Ullah I. Efficacy of Pasteuria penetrans on Meloidogyne incognita reproduction and growth of tomato. Pak J Zool. 2014;46(6):1651–55.
  22. Kumari NS. Role of bacterial bioagent, Pasteuria penetrans in the management of root-knot nematode, Meloidogyne incognita by altering the lifecycle. Pest Manag Hortic Ecosyst. 2017;23(1):76–79.
  23. Mhatre PH, Eapen SJ, Chawla G, Pervez R, Agisha V, Tadigiri S, Nagesh M. Isolation and characterization of Pasteuria parasitizing root-knot nematode, Meloidogyne incognita, from black pepper fields in India. Egypt J Biol Pest Control. 2020;30(1):1–7. https://doi.org/10.1186/s41938-020-00296-z
  24. Tzortzakakis E, Channer A, Gowen S, Ahmed R. Studies on the potential use of Pasteuria penetrans as a biocontrol agent of root?knot nematodes (Meloidogyne spp.). Plant Pathol. 1997;46(1):44–55. https://doi.org/10.1046/j.1365-3059.1997
  25. Chaudhary K, Kaul R. Efficacy of Pasteuria penetrans and various oil seed cakes in management of Meloidogyne incognita in Chilli pepper (Capsicum annuum L.). J Agric Sci Technol. 2013;15:617–26.
  26. Jena R, Basumatary B, Mohanta B, Pradhan S. Comparative efficacy of bio control agents against root knot nematode (Meloidogyne incognita) infecting brinjal. J Entomol Zool Stud. 2017;5(6):254–57.

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