Oilseeds are vital in India’s agriculture and nutrition, providing essential oils and proteins. They are a cornerstone of the edible oil industry, with India being one of the largest consumers globally and a major importer of edible oils from Indonesia and Malaysia. Investigations were carried out within the fifteen seed sources of Simarouba Glauca, to elucidate information on the best seed source in terms of storage potential and seed source. The results of the study on physical and physiological observations illustrated that Akola seed source performed better for hundred seed weight (98.64g), germination (75.75%), root length (12.42cm), shoot length (9.33cm), dry matter production (0.972g) and vigour index (75). The above results showed that among the seed variation, Akola seeds were resistant to adapt seed storage variability within 4, 8 and 12 months. This study thus provides valuable information for selecting and preserving Simarouba Glauca seeds for future planting and conservation efforts.