Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 12 No. 4 (2025)

Seed priming enhances chickpea (Cicer arietinum L.) germination and seedling establishment under varying thermal and moisture conditions

DOI
https://doi.org/10.14719/pst.7881
Submitted
22 February 2025
Published
06-10-2025 — Updated on 17-10-2025
Versions

Abstract

Seed priming is a unique technique used to improve seed performance in the field. It can overcome the major constraint of chickpea production in areas where farmers do not have enough resources to accelerate the seedbed preparation. This experiment investigated how different priming approaches; soil moisture regimes and soaking temperature affect the germination and seedling growth of chickpea. It was a three factorial experiment laid out in Completely Randomized Design (CRD) with three replications. Experimental factors were three priming conditions (non-priming, hydro-priming and osmo-priming), two soaking temperatures (21°C and 27 °C) and three soil moisture regimes [50 % field capacity (FC), 75 % FC and 90 % FC]. Among the three moisture regimes tested, osmo-primed chickpea seeds at 90 % FC achieved the highest germination (99.33 %), whereas non-primed seeds at 50 % FC showed the lowest germination (78.50 %). Osmo-priming at 21°C resulted in the highest germination percentage (87.89 %) and significantly increased root (12.8 cm) and shoot lengths (15.4 cm), as well as root (9.56 mg plant-1) and shoot weight (9.96 mg plant-1), whereas non-primed seeds at 27 °C showed the poorest performance across these parameters. The findings indicate that seed priming, especially osmo-priming at 21 °C under 90 % field capacity, is a promising strategy to improve germination efficiency and seedling establishment in chickpea.  

References

  1. 1. Jukanti AK, Gaur PM, Gowda CLL, Chibbar RN. Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review. British Journal of Nutrition. 2012;108:11-26. https://doi.org/10.1017/S0007114512000797
  2. 2. Food and Agriculture Organization (FAO). FAOSTAT Statistical Database. FAO; 2024.
  3. 3. Food and Agriculture Organization (FAO). Chickpea status of Bangladesh. Rome: Food and Agriculture Organization of United Nations; 2023.
  4. 4. Jisha VN, Smith RB, Pradeep S, Sreedevi S, Vnni KM, Sajith S, et al. Versatility of microbial proteases. Advances in Enzyme Research. 2013;1(3):39-51. https://doi.org/10.4236/aer.2013.13005
  5. 5. Lin JM, Sung JM. Pre-sowing treatment for improving emergence of bitter gourd seedling under optimal temperatures. Seed Science and Technology. 2001;29(1):39-50.
  6. 6. Basra SMA, Farooq M, Tabassum R, Ahmad N. Physiological and biochemical aspects of pre-sowing seed treatments in fine rice (Oryza sativa L.). Seed Science and Technology. 2005;33:623-8. https://doi.org/10.15258/sst.2005.33.3.09
  7. 7. Khalil SK, Mexal JG, Rehman A, Khan AZ, Wahab S, Zubair M, et al. Soybean mother plant exposure to temperature stress and its effect on germination under osmotic stress. Pakistan Journal of Botany. 2010;42(1):213-25.
  8. 8. Jeong H, Tombor B, Albert R, Oltvai ZN, Barabasi AL. The large-scale organization of metabolite networks. Nature. 2000;407(6804):651-4. https://doi.org/10.1038/35036627
  9. 9. Patade VY, Sujata B, Penna S. Halopriming imparts tolerance to salt & PEG induced drought stress in sugarcane. Agriculture, Ecosystems & Environment. 2009;134(1-2):24-8. https://doi.org/10.1016/j.agee.2009.07.003
  10. 10. Sammis TW, Al-Jamal MS, Ball S, Smeal D. Crop water use of onion. In: Proceedings of 6th International Micro Irrigation Congress. Micro 2000, South Africa; 2000. p. 1-9.
  11. 11. Kamithi DK, Kibe AM, Akuja TE. The effect of nitrogen and plant population on growth, yield and harvest index (HI) of chickpea (Cicer arietinum L.) under dry land condition of Kenya. Journal of Applied Biosciences. 2009;22:1359-67.
  12. 12. Hosseini NM, Palta JA, Berger JD, Siddique KHM. Sowing soil water content effects on chickpea (Cicer arietinum L.): seedling emergence and early growth interaction with genotype and seed size. Agricultural Water Management. 2009;96(12):1732-6. https://doi.org/10.1016/j.agwat.2009.07.010
  13. 13. Finch-Savage WE, Dent KC, Clark LJ. Soak conditions and temperature following sowing influence the response of maize (Zea mays L.) seeds to on-farm priming (pre-sowing seed soak). Field Crops Research. 2004;90(2-3):361-74. https://doi.org/10.1016/j.fcr.2004.04.006
  14. 14. Sehan-Gurtas F, Mehmet MAK, Evranuz EO. Water diffusion coefficients of selected legumes grown in Turkey as affected by temperature and variety. Turkish Journal of Agriculture. 2001;25(5):297-304.
  15. 15. Kader MA, Jutzi SC. Temperature, osmotic pressure and seed treatment influence imbibition rates in sorghum seeds. Journal of Agronomy and Crop Science. 2002;188(4):286-90. https://doi.org/10.1046/j.1439-037X.2002.00581.x
  16. 16. Rahman MM, Ahammad KU, Alam MM. Effect of soaking condition and temperature on imbibition rate of maize and chickpea seeds. Research Journal of Seed Science. 2011;4(2):117-24. https://doi.org/10.3923/rjss.2011.117.124
  17. 17. ISTA. Viability and vigour: evaluation and impact. 27th ISTA Congress, Seed Symposium, Budapest, Hungary; 2004.
  18. 18. Fiala F. Handbook of vigour test methods. Zurich: International Seed Testing Association; 1987. p. 28-36.
  19. 19. Duncan DB. Multiple range and multiple F tests. Biometrics. 1955;11:1-42. https://doi.org/10.2307/3001478
  20. 20. Ahammad KU, Rahman MM, Ali MR. Effect of hydropriming method on maize (Zea mays) seedling emergence. Bangladesh Journal of Agricultural Research. 2014;39(1):143-50. https://doi.org/10.3329/bjar.v39i1.20164
  21. 21. Lemmens E, Deleu LJ, De Brier N, De Man WL, De Proft M, Prinsen E, et al. The impact of hydro-priming and osmo-priming on seedling characteristics, plant hormone concentrations, activity of selected hydrolytic enzymes and cell wall and phytate hydrolysis in sprouted wheat (Triticum aestivum L.). ACS Omega. 2019;4(26):22089-100. https://doi.org/10.1021/acsomega.9b03210
  22. 22. Corbineau F, Taskiran-Özbingöl N, El-Maarouf-Bouteau H. Improvement of seed quality by priming: concept and biological basis. Seeds. 2023;2(1):101-15. https://doi.org/10.3390/seeds2010008
  23. 23. Gholami A, Shahsavani S, Nezarat S. The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. International Journal of Agricultural and Biosystems Engineering. 2009;3:9-14.
  24. 24. Beedi S, Macha SI, Gowda B, Savitha AS, Kurnallikar V. Effect of seed priming on germination percentage, shoot length, root length, seedling vigour index, moisture content and electrical conductivity in storage of kabuli chickpea cv. MNK-1 (Cicer arietinum L.). Journal of Pharmacognosy and Phytochemistry. 2018;7(1):2005-10.
  25. 25. Ren M, Tan B, Xu J, Yang Z, Zheng H, Tang Q, et al. Priming methods affected deterioration speed of primed rice seeds by regulating reactive oxygen species accumulation, seed respiration and starch degradation. Frontiers in Plant Science. 2023;14:1267103. https://doi.org/10.3389/fpls.2023.1267103
  26. 26. Fu JR, Lu RZ, Chen BZ, Zhang ZS, Lieu ZS, Li ZS, et al. Osmo conditioning of peanut seeds with PEG to improve vigor and some biochemical activities. Seed Science and Technology. 1988;16:197-212.
  27. 27. Afzal I, Basra SMA, Nazir A, Cheema MA, Warraich EA, Abdul K. Effect of priming and growth regulator treatment on emergence of seedling growth of hybrid maize (Zea mays L.). International Journal of Agricultural Biology. 2002;4(2):303-6.
  28. 28. Hardegree SP, Jones TA, Vactor SSV. Variability in thermal response of primed and non primed seeds of squirreltail [Elymus elymoides (Raf.) Swezey and Elymus multisetus (J.G. Smith) M.E. Jones]. Annals of Botany. 2002;89(3):311-9. https://doi.org/10.1093/aob/mcf043
  29. 29. Elkoca E, Haliloglu K, Esitken A, Ercisli S. Hydro- and osmopriming improve chickpea germination. Acta Agriculturae Scandinavica, Section B-Soil & Plant Science. 2007;57(3):193-200. https://doi.org/10.1080/09064710600914087
  30. 30. Al-Karaki GN. Response of wheat and barley during germination to seed osmopriming at different water potentials. Journal of Agronomy and Crop Science. 1998;181:229-35. https://doi.org/10.1111/j.1439-037X.1998.tb00422.x
  31. 31. Ahmadi A, Mardeh ASS, Poustini K, Jahromi ME. Influence of osmo and hydopriming on seed germination and seedling growth in wheat (Triticum aestivum L.) cultivars under different moisture and temperature conditions. Pakistan Journal of Biological Sciences. 2007;10:4043-9. https://doi.org/10.3923/pjbs.2007.4043.4049

Downloads

Download data is not yet available.