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Plant Science Today (PST)

Research Articles

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In vitro regeneration potential of Tecomella undulata using nodal and shoot tip explants

DOI
https://doi.org/10.14719/pst.8048
Submitted
3 March 2025
Published
04-08-2025
Versions

Abstract

Tecomella undulata (Sm.) is an important tree species widely used in India. The present study developed an efficient in vitro mass propagation protocol for Rohida. The study evaluated sterilization agents, media and plant growth regulators for the clonal propagation of Rohida using nodal and shoot-tip explants. The most successful sterilization treatment involved HgCl2 (0.1 %) for 3 min, followed by Bavistin (0.2 %) and Streptomycin (0.2 %) for 15 min. This protocol achieved 100 % explant survival. Among different media compositions, nodal explants showed the highest callus formation (73.3 ± 12.48) in EM7 (MS + BAP 2.0 mg/L + NAA 0.01 mg/L), while the control showed no callus formation. For shoot-tip explants, EM7 also resulted in the highest callus formation (86.6 ± 8.18 %) and the control produced no callus. EM10 (MS + BAP 2.5 mg/L + NAA 0.1 mg/L) led to the highest shoot initiation (86.7 ± 13.34 %) in nodal explants, with an average of 19 days for shoot induction. Similarly, EM10 produced the highest shoot initiation (93.3 ± 6.68) in shoot tip explants, with an average induction time of 11 days. The highest rooting percentage (66.6 %) was observed in EM4 (Half MS+ IBA 2.5 mg/L) with average of 40 days required for root induction. The survival rate of plantlets transferred to soil + vermiculite (1:1) potting mix under greenhouse conditions was 48 %. These findings provide valuable insights into optimizing propagation techniques for Tecomella undulata. In the current study, shoot tip explants proved to be superior to nodal explants for regeneration.

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