Nanotechnology holds significant potential in agriculture, contributing to various applications such as nanofertilizers, nanopesticides, nanoherbicides, nanosensors and more recently, electrospun nanofibers. Among such advancements, electrospinning has emerged as a versatile and cost-effective technique for fabricating nanofibers, offering significant potential to enhance sustainability in agricultural practices. Recent applications of electrospun fibers have primarily focused on drug delivery, wound dressings and seed coatings infused with growth hormones. These nanofibers exhibit notable properties such as a high surface-area-to-volume ratio, excellent porosity and the ability to facilitate controlled release of active compounds. Encapsulating microorganisms and agrochemicals within electrospun fibers offers an environmentally friendly approach to improve soil health. By forming a protective layer, the electrospun nanofibers help safeguard seeds against abiotic factors such as drought and temperature fluctuations, as well as biotic threats like pathogens and pests. Furthermore, embedding natural or synthetic antimicrobial agents into electrospun films offers an eco-friendly solution for post-harvest protection by effectively minimizing spoilage and the risk of pathogen invasion. This review emphasizes the diverse roles of electrospun nanofibers in sustainable agriculture, including soil enhancement, seed improvement and post-harvest protection, while also promoting eco-friendly practices using biodegradable polymers and bioactive agents.