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Plant Science Today (PST)

Research Articles

Early Access

Study on micropropagation and acclimatization of hippeastrum 'Double king' flowers in An Giang province of Vietnam

DOI
https://doi.org/10.14719/pst.9261
Submitted
2 May 2025
Published
27-08-2025
Versions

Abstract

Hippeastrum 'Double King' is a beautiful flower with high economic value and has recently been introduced to Vietnam; therefore, the process of producing this flower has not been systematically established. The study was conducted to find out the suitable media for the micropropagation process of the hippeastrum 'Double King' flowers and the multiplication of tissue-cultured plants in greenhouse conditions. In this study, four experiments were carried out, including shoot regeneration from bulb scales, in-vitro shoot multiplication, complete plantlet generation and evaluation of the adaptability and development of tissue-cultured seedlings in greenhouses. The result showed that MS medium supplemented with 2 mg/L BAP was the best medium for shoot regeneration and the two-scale explants produced the most shoots in this medium. For the shoot multiplication experiment, the quarter-bulb explants cultured in the MS medium supplemented with 0.5 mg/L NAA and 2 mg/L BAP produced the greatest number of shoots, the best plant height and the highest number of leaves per bulb explant. The in-vitro complete plantlet generation experiment showed that MS medium supplemented with 2 mg/L NAA, 0.5 g/L activated carbon and 40 g/L sucrose was the most suitable for rooting seedlings. The substrate of 75 % sand + 25 % rice husk
ash was suitable for the growth and development of seedlings in the nursery. The results of this study initially help to optimize the culture and production of 'Double King' hippeastrum flowers in An Giang province, Vietnam.

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