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Plant Science Today (PST)

Research Articles

Early Access

Comparison of functional properties of commercial plant protein isolates and sonication pretreatment assisted extraction-based plant protein isolate blends

DOI
https://doi.org/10.14719/pst.9360
Submitted
8 May 2025
Published
28-11-2025

Abstract

The current study was conducted at ICAR-Indian Agricultural Research Institute, New Delhi, India, in the year 2023. Plant Protein Isolates (PlPIs) derived from sesame, mung bean, peanut, chickpea and spirulina have attracted widespread attention due to their high nutritional value, the popularization of vegan diets and broad availability. However, their relatively poor functional properties compared to animal proteins limit their wider use in food formulations. This study investigates the effect of sonication pretreatment on the functional properties of lab-made PlPI blends compared to Commercial Plant Protein Isolates (CPlPIs). Functional attributes such as solubility, dispersibility, swelling capacity, foaming and gelation behaviour were evaluated. PlPI blends exhibited significantly higher solubility (up to ~48 % at pH 11) and oil-holding capacity (~339 %) than commercial isolates. Dispersibility in PlPI blends ranged narrowly from 61–63 %, whereas CPlPIs varied widely between 16 % and 100 %, depending on formulation. Swelling capacity was also notably improved in PlPI blends (13.3–426.9 %) compared to CPlPIs (12.1–122.2 %). Rheological analysis (flow properties) indicated shear-thinning behaviour (less viscous when stirred or subjected to force) for both PPI-blends and CPPIs, with PPI-blend 2 showing the highest viscosity due to its greater swelling capacity. PPI-blends demonstrated higher oil-holding capacity (330.6–339.7 %) than CPPIs (66.7–139.5 %). However, unlike CPlPIs, which formed gels at low concentrations (1–3 %), all PlPI blends required a higher concentration (12 %) to gel. This is the first study to systematically compare sonicated PlPI blends with CPlPIs across multiple functional traits using diverse plant sources. Overall, sonication pretreatment improved hydration and functional properties, making sonicated PlPI blends promising ingredients for developing plant-based dairy alternatives, meat analogues and other functional food products requiring improved solubility, foaming and texture.

References

  1. 1. Tang J, Yao D, Xia S, Cheong LZ, Tu M. Recent progress in plant-based proteins: from extraction and modification methods to applications in the food industry. Food Chem X. 2024;23:101540. https://doi.org/10.1016/j.fochx.2024.101540
  2. 2. Gao K, Rao J, Chen B. Unraveling the mechanism by which high intensity ultrasound improves the solubility of commercial pea protein isolates. Food Hydrocoll. 2022;131:107823. https://doi.org/10.1016/j.foodhyd.2022.107823
  3. 3. Fatima A, Singh P, Pandey VK, Singh R, Rustagi S. Exploring the significance of protein concentrate: a review on sources, extraction methods and applications. Food Chem Adv. 2024;5:100771. https://doi.org/10.1016/j.focha.2024.100771
  4. 4. Byanju B, Rahman MM, Hojilla-Evangelista MP, Lamsal BP. Effect of high-power sonication pretreatment on extraction and some physicochemical properties of proteins from chickpea, kidney bean and soybean. Int J Biol Macromol. 2020;145:712-21. https://doi.org/10.1016/j.ijbiomac.2019.12.118
  5. 5. Zhu X, Das RS, Bhavya ML, Garcia-Vaquero M, Tiwari BK. Acoustic cavitation for agri-food applications: mechanism of action, design of new systems, challenges and strategies for scale-up. Ultrason Sonochem. 2024:106850. https://doi.org/10.1016/j.ultsonch.2024.106850
  6. 6. Malik MA, Sharma HK, Saini CS. High intensity ultrasound treatment of protein isolate extracted from dephenolized sunflower meal: effect on physicochemical and functional properties. Ultrason Sonochem. 2017;39:511-19. https://doi.org/10.1016/j.ultsonch.2017.05.026
  7. 7. Naik M, Natarajan V, Modupalli N, Thangaraj S, Rawson A. Pulsed ultrasound assisted extraction of protein from defatted Bitter melon seeds (Momardica charantia L.) meal: kinetics and quality measurements. LWT. 2022;155:112997. https://doi.org/10.1016/j.lwt.2021.112997
  8. 8. Youshanlouei YA, Kiani H, Mousavi M, Mousavi ZE. Grass pea (Lathyrus sativus L.) protein yield and functionality as affected by extraction method: alkaline, ultrasound-assisted and ultrasound pretreatment extraction. Cereal Chem. 2022;99(4):931-46. https://doi.org/10.1002/cche.10549
  9. 9. Zou Y, Li PP, Zhang K, Wang L, Zhang MH, Sun ZL, et al. Effects of ultrasound-assisted alkaline extraction on the physiochemical and functional characteristics of chicken liver protein isolate. Poult Sci. 2017;96(8):2975-85. https://doi.org/10.3382/ps/pex049
  10. 10. Akbarbaglu Z, Ayaseh A, Ghanbarzadeh B, Sarabandi K. Techno-functional, biological and structural properties of Spirulina platensis peptides from different proteases. Algal Res. 2022;66:102755. https://doi.org/10.1016/j.algal.2022.102755
  11. 11. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-54. https://doi.org/10.1006/abio.1976.9999
  12. 12. Liu J, Shim YY, Shen J, Wang Y, Reaney MJ. Whey protein isolate and flaxseed (Linum usitatissimum L.) gum electrostatic coacervates: turbidity and rheology. Food Hydrocoll. 2017;64:18-27. https://doi.org/10.1016/j.foodhyd.2016.10.006
  13. 13. Kulkarni KD, Kulkarni DN, Ingle UM. Sorghum malt-based weaning food formulations: preparation, functional properties and nutritive value. Food Nutr Bull. 1991;13(4):1-7. https://doi.org/10.1177/156482659101300401
  14. 14. Jia W, Rodriguez-Alonso E, Bianeis M, Keppler JK, van der Goot AJ. Assessing functional properties of rapeseed protein concentrate versus isolate for food applications. Innov Food Sci Emerg Technol. 2021;68:102636. https://doi.org/10.1016/j.ifset.2021.102636
  15. 15. Lawal OS, Adebowale KO, Ogunsanwo BM, Sosanwo OA, Bankole SA. On the functional properties of globulin and albumin protein fractions and flours of African locust bean (Parkia biglobassa). Food Chem. 2005;92(4):681-91. https://doi.org/10.1016/j.foodchem.2004.08.043
  16. 16. Okezie BO, Bello AB. Physicochemical and functional properties of winged bean flour and isolate compared with soy isolate. J Food Sci. 1988;53(2):450-54. https://doi.org/10.1111/j.1365-2621.1988.tb07728.x
  17. 17. Venktesh A, Prakash V. Functional properties of the total proteins of sunflower (Helianthus annuus L.) seed-effect of physical and chemical treatments. J Agric Food Chem. 1993;41(1):18-23. https://doi.org/10.1021/jf00025a005
  18. 18. Guldiken B, Stobbs J, Nickerson M. Heat induced gelation of pulse protein networks. Food Chem. 2021;350:129158. https://doi.org/10.1016/j.foodchem.2021.129158
  19. 19. Li M, Wu J, Chen Z, Wu T. Improving the solubility of myofibrillar proteins (MPs) by mixing with sodium alginate: effects of pH, mixing ratios and preheating of MPs. Food Biophys. 2020;15:113-21. https://doi.org/10.1007/s11483-019-09605-y
  20. 20. Loveday SM, Halim C. Protein isolates made in the laboratory versus the factory: a case study with mung bean and chickpea. Sustain Food Proteins. 2024;2(2):89-98. https://doi.org/10.1002/sfp2.1027
  21. 21. Linke C, Drusch S. Turbidity in oil-in-water-emulsions - key factors and visual perception. Food Res Int. 2016;89:202-10. https://doi.org/10.1016/j.foodres.2016.07.019
  22. 22. Zhu Z, Bassey AP, Cao Y, Ma Y, Huang M, Yang H. Food protein aggregation and its application. Food Res Int. 2022;160:111725. https://doi.org/10.1016/j.foodres.2022.111725
  23. 23. Kinsella JE. Functional properties of soy proteins. J Am Oil Chem Soc. 1979;56:242-58. https://doi.org/10.1007/BF02671468
  24. 24. Manzocco L, Plazzotta S, Powell J, de Vries A, Rousseau D, Calligaris S. Structural characterisation and sorption capability of whey protein aerogels obtained by freeze-drying or supercritical drying. Food Hydrocoll. 2022;122:107117. https://doi.org/10.1016/j.foodhyd.2021.107117
  25. 25. Żmudziński D, Goik U, Ptaszek P. Functional and rheological properties of Vicia faba L. protein isolates. Biomolecules. 2021;11(2):178. https://doi.org/10.3390/biom11020178
  26. 26. Webb D, Dogan H, Li Y, Alavi S. Physico-chemical properties and texturization of pea, wheat and soy proteins using extrusion and their application in plant-based meat. Foods. 2023;12(8):1586. https://doi.org/10.3390/foods12081586
  27. 27. Stone AK, Avarmenko NA, Warkentin TD, Nickerson MT. Functional properties of protein isolates from different pea cultivars. Food Sci Biotechnol. 2015;24:827-33. https://doi.org/10.1007/s10068-015-0107-y
  28. 28. Mahomud MS, Katsuno N, Nishizu T. Formation of soluble protein complexes and yoghurt properties influenced by the addition of whey protein concentrate. Innov Food Sci Emerg Technol. 2017;42:173-80. https://doi.org/10.1016/j.ifset.2017.05.010
  29. 29. Yan J, Zhao S, Xu X, Liu F. Enhancing pea protein isolate functionality: a comparative study of high-pressure homogenization, ultrasonic treatment and combined processing techniques. Curr Res Food Sci. 2024;8:100653. https://doi.org/10.1016/j.crfs.2023.100653
  30. 30. Xu X, Tao J, Wang Q, Ge J, Li J, Gao F, et al. A comparative study: functional, thermal and digestive properties of cereal and leguminous proteins in ten crop varieties. LWT. 2023;176:115288. https://doi.org/10.1016/j.lwt.2023.115288
  31. 31. Cano-Medina A, Jiménez-Islas H, Dendooven L, Herrera RP, González-Alatorre G, Escamilla-Silva EM. Emulsifying and foaming capacity and emulsion and foam stability of sesame protein concentrates. Food Res Int. 2011;44(3):684-92. https://doi.org/10.1016/j.foodres.2010.12.015
  32. 32. Ma Y, Fu S, Yang S, Chen F, Cheng KW, Liu B. Nutritional and functional properties of photosynthetic microorganism proteins: a comparison with soy protein isolate and wheat protein. LWT. 2024;177:115920. https://doi.org/10.1016/j.lwt.2024.115920
  33. 33. Delahaije RJ, Wierenga PA. Hydrophobicity enhances the formation of protein-stabilized foams. Molecules. 2022;27(7):2358. https://doi.org/10.3390/molecules27072358
  34. 34. Moreno HM, Dominguez-Timon F, Díaz MT, Pedrosa MM, Borderías AJ, Tovar CA. Evaluation of gels made with different commercial pea protein isolate: rheological, structural and functional properties. Food Hydrocoll. 2020;106:105375. https://doi.org/10.1016/j.foodhyd.2019.105375
  35. 35. Idowu AO, Alashi AM, Nwachukwu ID, Fagbemi TN, Aluko RE. Functional properties of sesame (Sesamum indicum Linn) seed protein fractions. Food Prod Process Nutr. 2021:1-16. https://doi.org/10.1186/s43014-020-00047-5
  36. 36. Lawal OS. Functionality of African locust bean (Parkia biglobossa) protein isolate: effects of pH, ionic strength and various protein concentrations. Food Chem. 2004:345-55. https://doi.org/10.1016/j.foodchem.2003.09.036

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