Generative Pre-trained Transformers (GPTs) are transforming agricultural science education by enhancing students' critical thinking, creativity and decision-making skills. However, concerns exist regarding over-reliance and diminished independent analytical thinking. This study investigated the impact of GPT usage among undergraduate and postgraduate students at Tamil Nadu Agricultural University. A structured questionnaire was employed to collect primary data from 164 students (122 GPT users and 42 non-users). The research adopts a descriptive design and utilizes statistical tools such as percentage analysis, Garrett ranking, Relative Importance Index (RII) and exploratory factor analysis to assess the influence of GPTs on cognitive skills. The findings indicate that the GPTs enhance efficiency, creativity and problem-solving, they additionally possess the risk of overreliance, reducing independent analytical thinking. Factor analysis revealed key dimensions, including decision-making enhancement, overreliance on AI and the impact on problem solving of agricultural science students. The study concludes that balanced AI integration is essential for maximizing GPT's advantages while minimizing dependency. Agricultural institutions should promote critical engagement strategies to ensure responsible AI use and foster an optimal AI-human synergy in the academic learning process of agricultural students.