The aim of evaluating the mycelial development and production of Pleurotus spp. to various botanical extracts in the laboratory. The effects of the botanicals evaluated on Petri plates on Pleurotus sapidus and Pleurotus flabellatus fruiting body formation and spawn growth. All plant extracts were found to be supportive of mushroom mycelial growth. P. sapidus showed the highest yield, 861.25 g/kg dry substrate at 86.12 % biological efficiency (BE) with minimum days for spawn run were observed (14.25 days), minimum days for first harvesting (21.25 days) and maximum numbers of fruiting bodies (65.75) were observed in wheat straw + Lantana camara foliage powder at 4.0 %. In case of P. flabellatus, the highest yield of 878.25 g/kg dry matter at 87.82 % BE with the minimum number of days for spawn run was recorded in 13.50 days. The minimum days for first harvesting were 20.50 days and the highest number of fruiting bodies (65.50) was noticed in the medium of wheat straw + L. camara leaf powder at 4.0 %. Eucalyptus was found to be comparatively less effective for Pleurotus spp. The L. camara foliage extract and powder were the highest growth promoters of mycelium and increasing BE and can be adopted to enhance the production of oyster mushroom cultivation. The botanicals used in the study are easily available for the growers and directly impact to their economy.