This study evaluated the impact of various organic amendments on yield, nutrient uptake and protein content in mung bean (Vigna radiata L.) during the Kharif 2022 in a randomized block design (RBD) with four replications and eleven treatments. The results indicated that the application of TRDF + R + J (Recommended dose of fertilizers + Rhizobium + Jeevamrutam) significantly increased the grain yield compared to TRDF (Recommended dose of fertilizers) and was statistically at par with TRDF + R (Recommended dose of fertilizers + Rhizobium). However, the treatment TRDF + V + R + J (Recommended dose of fertilizers + Vermicompost + Rhizobium + Jeevamrutam) resulted in a significantly higher grain yield compared to TRDF + V + R (Recommended dose of fertilizers + Vermicompost + Rhizobium). The combined application of organic manures specifically a mixture of vermicompost, Rhizobium and Jeevamrutam (TV+R+J) significantly enhanced nitrogen (N) and phosphorus (P) uptake in both grain and straw compared to individual applications. Notably, the application of recommended doses of fertilizers (TRDF) yielded higher N uptake in grain relative to Jeevamrutam and vermicompost alone, but did not surpass the combined organic amendment application. Enhanced N and P uptake was also observed with (TRDF) in conjunction with organic amendments, particularly when combined with Rhizobium and vermicompost. Furthermore, protein content in mung bean grains significantly increased with the application of (TV+R+J), outperforming single applications of Rhizobium, Jeevamrutam and vermicompost. While (TRDF) alone raised protein levels compared to Jeevamrutam, it was not significantly different from (TV+R+J). The study concludes that integrated organic amendments provide superior benefits in nutrient uptake and protein enhancement in mung bean, suggesting a sustainable approach to improve crop yield and nutritional quality. These findings underscore the importance of combining organic and inorganic fertilizers for optimal mung bean cultivation.