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Plant Science Today (PST)

Research Articles

Vol. 13 No. 2 (2026)

Antioxidant, anticoagulant and antihyperlipidemic activities of Thymus willdenowii Boiss. collected from three different harvesting sites in Eastern High Atlas

DOI
https://doi.org/10.14719/pst.10621
Submitted
13 July 2025
Published
14-05-2026 — Updated on 23-05-2026
Versions

Abstract

Thyme species are commonly used in Moroccan folk medicine to prevent and treat cardiovascular diseases and their risk factors. In this respect, Thymus willdenowii Boiss. collected from 3 different harvesting site in Eastern High Atlas (Outerbate, Tissila and Aït Ali Ou Ikkou) was evaluated and compared for its antioxidant, anticoagulant and antihyperlipidemic activities.The antioxidant activity was assessed through 2,2-diphenyl-1-picrylhydrazyle (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing ability of plasma (FRAP) methods. The anticoagulant activity was evaluated based on prothrombin time (PT) and activated partial thromboplastin time (aPTT). While the antihyperlipidemic effect was examined using Triton-1339-induced hyperlipidemia in rats. The Tissila extract had the most phenolic (116.74 ± 0.52 mg GAE/g) and flavonoid (16.73 ± 0.9 mg RE/g) content, which was linked to better antioxidant activity, with IC50 values of 66.45 µg/mL for DPPH and 20.53 µg/mL for ABTS.  In coagulation assays, the Tissila extract exhibited the most pronounced effect, extending PT to 25.13 sec at 16.29 µg/mL, in contrast to 12.37 sec in the control.  Additionally, in hyperlipidemic rats, the Tissila extract (400 mg/kg) elicited the most significant biological response, decreasing total cholesterol by 63 %, LDL-C by 62 %  and the atherogenic index by 84 %, while elevating HDL-C levels by 121 %. On the other hand, the Outerbate extract did not have a strong effect on lowering blood lipids. Findings suggest the potential of T. willdenowii as a source of natural antioxidant and cardioprotective agents.

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