Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Early Access

Influence of light emitting diodes as alternative lighting for cabbage (Brassica oleracea var. capitata) microgreens grown under ambient conditions

DOI
https://doi.org/10.14719/pst.10659
Submitted
15 July 2025
Published
08-01-2026

Abstract

Artificial lighting is essential in indoor farming, wherein light quality is considered in crop production. In this study, light-emitting diodes (LEDs) using different colours (red (R), blue (B), RB and white (as control) were used to determine the impact on the growth of cabbage microgreens under ambient conditions. A completely randomised design with 4 replications was used. Microgreens were exposed to LEDs for 10 hr and 14 hr in darkness. The microgreens were harvested 10 days after emergence by cutting them from the surface of vermicast (as the substrate) using a sterilised pair of scissors. Results showed that white LED had shorter microgreens and hypocotyls than the other LEDs. Whereas the leaves were longer by exposing the microgreens to blue and RB LEDs than the white LED, the former LEDs showed a similarity with the red LED. A high root count was observed in microgreens with blue and RB LEDs, which was higher than red LEDs. But the white LED showed a similar root count to the other LEDs. Moreover, white LED produced a higher chlorophyll content based on its high SPAD index. Red and RB LEDs produced a higher fresh weight of microgreens than white LEDs. Fresh weight per microgreen had a positive relationship with the fresh weight yield of microgreens. Likewise, microgreen height, hypocotyl length and leaf length displayed a positive and significant association with a high yield of cabbage microgreens. Either red or RB LED is recommended to obtain a higher yield of microgreens for ambient indoor growing conditions.

References

  1. 1. Bhaswant M, Shandmugam D, Miyazawa T, Abe C, Miyazawa T. Microgreens-a comprehensive review of bioactive molecules and health benefits. Molecules. 2023;28:867. https://doi.org/10.3390/molecules28020867
  2. 2. Zhang Y, Xiao Z, Ager E, Kong L, Tan L. Nutritional quality and health benefits of microgreens, a crop of modern agriculture. J Future Foods. 2021;1:58-66. https://doi.org/10.1016/j.jfutfo.2021.07.001
  3. 3. Chunthawodtiporn J, Kuengsaard T, Manochai B, Pornwongthong P. Nutritional properties of nine microgreens consumed in Thailand. Appl Sci Eng Prog. 2023;16:1-9.
  4. 4. Chen M, Chory J, Fankhauser C. Light signal transduction in higher plants. Ann Rev Genet. 2004;38:87-117. https://doi.org/10.1146/annurev.genet.38.072902.092259
  5. 5. Kreslavskii V, Los D, Schmitt F, Zharmukhamedov S, Kuznetsov V, Allakhverdiev S. The impact of the phytohormones on photosynthetic processes. Biochimica et Biophysica Acta. 2018;1859:400-8. https://doi.org/10.1016/j.bbabio.2018.03.003
  6. 6. Kong Y, Zheng Y. Magic blue light: a versatile mediator of plant elongation. Plants. 2023;13:115. https://doi.org/10.3390/plants13010115
  7. 7. Fukuda N, Ajima C, Yukawa T. Antagonistic action of blue and red light on shoot elongation in petunia depends on gibberellin, but the effects on flowering are not generally linked to gibberellin. Environ Exp Bot. 2016;121:102-11. https://doi.org/10.1016/j.envexpbot.2015.06.014
  8. 8. Lu N, Maruo T, Johkan M, Hohjo M, Tsukakoshi S, Ito Y, et al. Effects of supplemental lighting with light-emitting diodes (LEDs) on tomato yield and quality of single-truss tomato plants grown at high planting density. Environ Control Biol. 2012;50:63-74. https://doi.org/10.2525/ecb.50.63
  9. 9. Wang H, Gu M, Cui J, Shi K, Zhou Y, Yu J. Effects of light quality on CO₂ assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J Photochem Photobiol B: Biology. 2009;96:30-7. https://doi.org/10.1016/j.jphotobiol.2009.03.010
  10. 10. Son K, Oh M. Leaf shape, growth and antioxidant phenolic compounds of two lettuce cultivars grown under various combinations of blue and red light-emitting diodes. HortScience. 2013;48:988-95. https://doi.org/10.21273/HORTSCI.48.8.988
  11. 11. Izzo L, Mele B, Vitale L, Vitale E, Arena C. The role of monochromatic red and blue light in tomato early photomorphogenesis and photosynthetic traits. Environmental and Experimental Botany. 2020;179:104195. https://doi.org/10.1016/j.envexpbot.2020.104195
  12. 12. Association of Official Analytical Chemists. Official methods of analysis AOAC International. 17th ed. Maryland: Association of Official Analytical Chemists; 2000.
  13. 13. Spaninks K, van Lieshout J, van Leperen W, Offringa R. Regulation of early plant development by red and blue light: a comparative analysis between Arabidopsis thaliana and Solanum lycopersicum. Front Plant Sci. 2020;11. https://doi.org/10.3389/fpls.2020.599982
  14. 14. Ponno J, Hoecker U. Signaling mechanisms by Arabidopsis cryptochromes. Front Plant Sci. 2022;13:844714. https://doi.org/10.3389/fpls.2022.844714
  15. 15. Ohashi-Kaneki K, Takase M, Kon N, Fujiwara K, Kurata K. Effect of light quality on growth and vegetable quality in leaf lettuce, spinach and komatsuna. Environ Control Biol. 2007;45:189-98. https://doi.org/10.2525/ecb.45.189
  16. 16. Huche-Thelier L, Crespel J, Le Gourriecrec J, Morel P, Sakr S, Leduc N. Light signaling and plant responses to blue and UV radiations-perspectives for applications in horticulture. Environ Exp Bot. 2016;121:22-38. https://doi.org/10.1016/j.envexpbot.2015.06.009
  17. 17. Gomez C, Izzo L. Increasing efficiency of crop production with LEDs. AIMS Agri Food. 2018;3:135-53. https://doi.org/10.3934/agrfood.2018.2.135
  18. 18. Kusuma P, Pattison P, Bugbee B. From physics to fixtures to food: current and potential LED efficacy. Hort Res. 2020;7. https://doi.org/10.1038/s41438-020-0283-7
  19. 19. Kong Y, Kamath D, Zheng Y. Blue versus red light can promote elongation growth independent of photoperiod: a study in four Brassica microgreens species. HortSci. 2019;54:1955-61. https://doi.org/10.21273/HORTSCI14286-19
  20. 20. Fukuda N, Yoshida T, Olsen J, Senaha C, Jikukaru Y, Kamiya Y. Short main shoot length and inhibition of floral bud development under red light can be recovered by application of gibberellin and cytokinin. Acta Hort. 2013;956:215-22. https://doi.org/10.17660/ActaHortic.2012.956.23
  21. 21. Wang S, Liu X, Liu X, Xue J, Ren X, Zhai Y, Zhang X. The red/blue light ratios from light-emitting diodes affect growth and flower quality of Hippeastrum hydridum ‘Red Lion’. Front Plant Sci. 2022;13. https://doi.org/10.3389/fpls.2022.1048770
  22. 22. Toscano S, Cavallaro V, Ferrante A, Romano D, Patane C. Effects of different light spectra on final biomass production and nutritional quality of two microgreens. Plants. 2021;10. https://doi.org/10.3390/plants10081584
  23. 23. Fermin J, Rosales R, Gregorio-Balbas M, Pungtilan A, Balbas C. Carbonized rice hull and vermiculite as substrates for indoor farming of pechay microgreens. Eco Envi Conserv. 2025;31:27-32. https://doi.org/10.53550/EEC.2025.v31i01.004
  24. 24. Rabago A, Rosales R, Gregorio-Balbas M, Pungtilan A. Utilization of locally available substrates and their effect on the growth and yield of cauliflower (Brassica oleracea botrytis group) microgreens. Basrah J Agric Sci. 2024;37:276-87. https://doi.org/10.37077/25200860.2024.37.2.21

Downloads

Download data is not yet available.