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Plant Science Today (PST)

Research Articles

Vol. 12 No. sp4 (2025): Recent Advances in Agriculture by Young Minds - III

GC-MS based phytochemical profiling of bhendi hybrid CO4 leaves under Nano DAP treatment

DOI
https://doi.org/10.14719/pst.10793
Submitted
21 July 2025
Published
17-10-2025

Abstract

Abelmoschus esculentus L. (bhendi or okra) is widely cultivated for its nutritional, economic and therapeutic value. This study investigates the phytochemical composition of bhendi hybrid CO4 leaves treated with Nano diammonium phosphate (Nano DAP) using Gas Chromatography-Mass Spectrometry (GC-MS). Comparative profiling identified 44 metabolites in Nano DAP-treated samples compared with 37 metabolites in untreated control. The identified compounds include hydrocarbons, esters, alcohols, diterpenes and fatty acid methyl esters, reflecting diverse metabolic activities. Notably, compounds such as neophytadiene (13.93 %), isopropyl myristate (13.63 %), squalene (7.23 %) and 9, 12, 15-octadecatrienoic acid methyl ester (5.16 %) were detected in high abundance, known for their antioxidant, anti-inflammatory and antimicrobial properties. The nano DAP application triggered the biosynthesis of several unique metabolites do not present in control samples, indicating enhanced secondary metabolism. The enriched terpenoid and lipid profiles suggest a shift in biochemical pathways associated with nutrient assimilation, oxidative stress mitigation and defence mechanisms. These findings highlight the potential of nano-based phosphorus delivery to modulate metabolic dynamics and promote crop resilience in bhendi. This study establishes a foundation for further research into nano-fertilizer-mediated metabolomic shifts and their role in improving vegetable crop physiology and phytochemical richness.

References

  1. 1. Kah M, Tufenkji N, White JC. Nano-enabled strategies to enhance crop nutrition and protection. Nature nanotechnology. 2019;14(6):532-40. https://doi.org/10.1038/s41565-019-0439-5
  2. 2. Tarafdar J, Raliya R, Rathore I. Microbial synthesis of phosphorous nanoparticle from tri-calcium phosphate using Aspergillus tubingensis TFR-5. J Bionanosci. 2012;6(2):84-9. https://doi.org/10.1166/jbns.2012.1077
  3. 3. ul Ain Q, Hussain HA, Zhang Q, Rasheed A, Imran A, Hussain S, et al. Use of nano-fertilizers to improve the nutrient use efficiencies in plants. In: Sustainable plant nutrition. Elsevier. 2023. p. 299-321. https://doi.org/10.1016/B978-0-443-18675-2.00013-4
  4. 4. Kumar A, Ram H, Kumar S, Kumar R, Yadav A, Gairola A, et al. A comprehensive review of nano-urea vs. conventional urea. Int J Plant Soil Sci. 2023;35:32-40. https://doi.org/10.9734/ijpss/2023/v35i153070
  5. 5. Zhao L, Hu J, Huang Y, Wang H, Adeleye A, Ortiz C, et al. 1H NMR and GC–MS based metabolomics reveal nano-Cu altered cucumber (Cucumis sativus) fruit nutritional supply. Plant Physiol Biochem. 2017;110:138-46. https://doi.org/10.1016/j.plaphy.2016.02.010
  6. 6. Ohlrogge J, Browse J. Lipid biosynthesis. The plant cell. 1995;7(7):957. https://doi.org/10.1105/tpc.7.7.957
  7. 7. Bhardwaj M, Sali VK, Mani S, Vasanthi HR. Neophytadiene from Turbinaria ornata suppresses LPS-induced inflammatory response in RAW 264.7 macrophages and Sprague Dawley rats. Inflammation. 2020;43(3):937-50. https://doi.org/10.1007/s10753-020-01179-z
  8. 8. Wang X, Kong L, Zhi P, Chang C. Update on cuticular wax biosynthesis and its roles in plant disease resistance. Int J Mol Sci. 2020;21(15):5514. https://doi.org/10.3390/ijms21155514
  9. 9. Reddy LH, Couvreur P. Squalene: A natural triterpene for use in disease management and therapy. Advanced drug delivery reviews. 2009;61(15):1412-26. https://doi.org/10.1016/j.addr.2009.09.005
  10. 10. Mène-Saffrané L. Vitamin E biosynthesis and its regulation in plants. Antioxidants. 2017;7(1):2. https://doi.org/10.3390/antiox7010002
  11. 11. Abd-Elsalam KA, Mohamed HI. Plant growth regulators to manage biotic and abiotic stress in agroecosystems: CRC Press Boca Raton. 2024. https://doi.org/10.1201/9781003389507
  12. 12. Wasternack C, Strnad M. Jasmonates are signals in the biosynthesis of secondary metabolites-Pathways, transcription factors and applied aspects-A brief review. New biotechnology. 2019;48:1-11. https://doi.org/10.1016/j.nbt.2017.09.007
  13. 13. Kumar S, Abedin MM, Singh AK, Das S. Role of phenolic compounds in plant-defensive mechanisms. Plant phenolics in sustainable agriculture: volume 1. Springer. 2020. p. 517-32. https://doi.org/10.1007/978-981-15-4890-1_22

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