Cropping systems used in agriculture impact soil health and quality and were initially designed to maximise yield from agricultural systems. However, modern agriculture has become increasingly concerned about the environmental sustainability of these systems. Soil samples were collected from different crop fields under conservation agriculture ecology at Bihar Agricultural University, Sabour, Bhagalpur. The soil samples were collected to a depth of 15 cm and then stored in small, sterile polythene bags for further laboratory studies. During the investigation period, 168 fungal colonies out of 13 fungal species and 6 genera of fungi were recorded. The highest number of fungal species recorded belongs to the phylum Deuteromycotina (159 colonies) and Zygomycotina (7 colonies). The isolation of soil mycoflora was carried out by using methodology, viz., the soil dilution technique and the soil plate technique on Potato dextrose agar (PDA) media and Czapeks’ Dox agar (CZA) media supplemented with suitable antibiotics. Identification and characterisation of the mycoflora were made by microscopic morphological characters with the help of a microscope. The fungi identified, isolated and characterised were Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Curvularia clavata, Curvularia lunata, Fusarium oxysporum, Fusarium solani, Penicillium chrysogenum, Penicillium frequentans, Penicillium funiculosum, Trichoderma harzianum, Trichoderma viride and Rhizopus stolonifer. The variations in soil mycoflora and percentage frequency were recorded.