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Plant Science Today (PST)

Research Articles

Vol. 12 No. 4 (2025)

Bulb maturity and storage effect on total phenolic and flavonoid contents and antioxidant activity of freeze-dried garlic

DOI
https://doi.org/10.14719/pst.10952
Submitted
29 July 2025
Published
10-11-2025 — Updated on 27-11-2025
Versions

Abstract

A study on the morphological characterization of one local garlic variety was conducted.  The effects of various maturity levels and storage were evaluated via measurement of parameters such as Total Phenolic Content (TPC), Total Flavonoid Contents (TFC) and antioxidant activity. The assessments were done in plants divided into four groups depending upon the period of maturity i.e. 90, 100, 110 and 120 Days After Planting (DAP) and three storage periods i.e. 30, 60 and 90 days under ambient conditions. Results showed that bulb weight, bulb diameter and clove weight increased as the garlic matured. These morphological characteristics showed highest values at maturity achieved at 120 DAP. Before storage, TPC and TFC were not affected by maturity, irrespective of the group. The antioxidant activity was higher at 90 and 110 DAP.  After storage, maturity group and storage period showed a significant effect on the TPC, TFC and antioxidant activity. TPC was significantly decreased after storage as compared to without storage. Values of the parameters were highest at maturity achieved at 100 and 120 DAP. Whereas storage exhibited a decline in contents across maturity groups in the case of TFC. In 90 to 120 DAP, a decreasing trend of the TFC was observed from 30 to 90 days of storage. A 120 DAP maturity and storage for 90 days is recommended for the farmers to obtain bigger bulbs and cloves due to its higher weight and higher TPC and TFC and comparable antioxidant activity with the other maturity groups. Analysis of other secondary metabolites is recommended. 

References

  1. 1. Tresina P, Selvam M, Doss A, Mohan V. Antidiabetic bioactive natural products from medicinal plants. Stud Nat Prod Chem. 2022;75:75-118. https://doi.org/10.1016/B978-0-323-91250-1.00004-5
  2. 2. Castaneda W, Igarta S, Baligat J, Aragon C, Billedo N, Paller R. Technology and investment profile of improved native garlic. SEARCA & DA-BAR; 2020.
  3. 3. Shang A, Xu X, Gan R, Tang G, Corke H, Mavumengwana V, et al. Bioactive compounds and biological functions of garlic (Allium sativum L.). Foods. 2019;8:246. https://doi.org/10.3390/foods8070246
  4. 4. Ren F, Zhou S. Phenolic components and health beneficial properties of onions. Agriculture. 2021;11:872. https://doi.org/10.3390/agriculture11090872
  5. 5. Ghimire B, Seo J, Kim S, Gimire B, Lee J, Yu C, et al. Influence of harvesting time on phenolic and mineral profiles and their association with the antioxidant and cytotoxic effects of Atractylodes japonica Koidz. Agronomy. 2021;11:1327. https://doi.org/10.3390/agronomy11071327
  6. 6. Skoczylas J, Jedrszczyk E, Dziadek K, Dacewicz E, Kopec A. Basic chemical composition, antioxidant activity and selected polyphenolic compounds profile in garlic leaves and bulbs collected at various stages of development. Molecules. 2023;28:6653. https://doi.org/10.3390/molecules28186653
  7. 7. Bhatta S, Janezic T, Ratti C. Freeze-drying of plant-based foods. Foods. 2020;9:87.https://doi.org/10.3390/foods9010087
  8. 8. Gong H, Wang T, Wang W, Shi C, Xu H, Li L, et al. Garlic varieties and drying methods affected the physical properties, bioactive compounds and antioxidant capacity of dried garlic powder. CYTA J Food. 2022;20:111-9. https://doi.org/10.1080/19476337.2022.2093400
  9. 9. Tedeschi P, Brugnoli F, Merighi S, Grassilli S, Nigro M, Catani M, et al. The effect of different storage conditions in phytochemical composition, shelf life and bioactive compounds of Voghiera garlic PDO. Antioxidants. 2023;12:499. https://doi.org/10.3390/antiox12020499
  10. 10. Ladeska V, Elya B, Hanafi M. Antioxidants, total phenolic and flavonoid content and toxicity assay of ampleas (Tetracera macrophylla Wall. ex Hook. f. & Thoms) from Kalimantan-Indonesia. Pharmacogn J. 2022;14:642-8. https://doi.org/10.5530/pj.2022.14.147
  11. 11. Desta B, Woldetsadik K, Ali W. Effect of harvesting time, curing and storage methods on storability of garlic bulbs. Open Biotechnol J. 2021;15:36-45. https://doi.org/10.2174/1874070702115010036
  12. 12. Lu Q, Li L, Xue S, Yang D, Wang S. Stability of flavonoid, carotenoid, soluble sugar and vitamin C in 'Cara Cara' juice during storage. Foods. 2019;8:417. https://doi.org/10.3390/foods8090417
  13. 13. Pudzianowska M, Gajewski M, Przybyl J, Buraczynska A, Gaczkowska O, Matuszczak M, et al. Influence of storage conditions on flavonoids content and antioxidant activity of selected shallot (Allium cepa var. ascalonicum Backer) hybrid cultivars. Veg Crops Res Bull. 2012;77:101-11.https://doi.org/10.2478/v10032-012-0019-9
  14. 14. Senami N, Bedouhene S, Rekeb T, Bouadjela L. Effect of freezing and drying on the bioactive compounds and antioxidant potential of garlic. Bulg J Agric Sci. 2024;30:1128-35.
  15. 15. Al-Dabbas M, Joudeh K, Abughoush M, Al-Dalali S. Effect of processing and preservation methods on total phenolic contents and antioxidant activities of garlic (Allium sativum). J Chil Chem Soc. 2023;68:5822-26. https://doi.org/10.4067/s0717-97072023000205822
  16. 16. Zhang X, Li N, Lu X, Liu P, Qiao X. Effects of temperature on the quality of black garlic. J Sci Food Agric. 2016;96:2366-72. https://doi.org/10.1002/jsfa.7351
  17. 17. Sunanta P, Kontogiorgos V, Pankasemsuk T, Jantanasakulwong K, Rachtanapun P, Seesuriyachan P, et al. The nutritional value, bioactive availability and functional properties of garlic and its related products during processing. Front Nutr. 2023;10:1142784. https://doi.org/10.3389/fnut.2023.1142784
  18. 18. Wilson D, Nash P, Buttar H, Griffiths K, Singh R, De Meester F, et al. The role of food antioxidants, benefits of functional foods and influence of feeding habits on the health of the older person: an overview. Antioxidants. 2017;6:81. https://doi.org/10.3390/antiox6040081

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