Phenotypic and genetic characterization of date palm cultivars resistant to bayoud disease
DOI:
https://doi.org/10.14719/pst.2021.8.4.1105Keywords:
Algeria, Taqerbucht cultivars, Date morphological traits, SSR markers, Sequence comparisonsAbstract
Taqerbucht cultivars of date palm are well known by their natural resistance against devastating fungus Bayoud disease. In order to know, if these accessions have the same genetic and morphological profile or each of them constitutes a separate cultivar, we carried out a morphological and molecular characterization and we compared four Taqerbucht (Tq.) date palm cultivars from the southwestern region of Algeria: Tq.hamra cultivar (red fruits), Tq. safra cultivar (yellow fruits), Tq.beïda (white fruits) and Tq.kahla cultivar (black fruits). Seventy one phenotypic characteristics, including 33 quantitative and 38 qualitative traits, have been selected for comparison. Principal component analysis (PCA) and multi-component clustering were used to analyze and compare the data. The results suggest that the four cultivars can be classified into distinct groups. One group contains one cultivar, the Tq.kahla and another group contains the three other cultivars (Tq.safra, Tq.beïda and Tq. hamra). Based on phylogenetic analyses and sequence comparisons, the cultivar Tq. kahla seems to be divergent from the cultivar Tq.hamra, whereas the two cultivars Tq.Safra and Tq.beïda are close to each other. Using 16 Simple Sequence Repeat (SSR) genetic markers to analyze genetic diversity among the cultivars, we found that 13 markers were detectable in 31 allele's loci, and the number of alleles per locus varied from 1–4 with an average of 2.38 alleles per locus. Expected heterozygosity (He) values ranged from 0.375–0.500 and observed heterozygosity (Ho) values from 0.750–1.000.
Downloads
References
Djaafri M, Kalloum S, Kaidi K, Salem F, Balla S, Meslem D and Iddou. Enhanced Methane Production from Dry Leaflets of Algerian Date Palm (Phoenix dactylifera L.) Hmira Cultivar, by Alkaline Pretreatment. Waste and Biomass Valorization 2020;11:2661–71. https://doi.org/10.1007/s12649-018-00574-w
Hannachi, S. Ressources génétiques du palmier dattier (Phoenix dactylifera L.) en Algérie : Analyse de la variabilité inter et intra des principaux cultivars. Thèse Magister. ENSA Alger; 2012. Available from: http://hdl.handle.net/123456789/1889
Farooq S, Maqbool MM, Bashir MA et al. Production suitability of date palm under changing climate in a semi-arid region predicted by CLIMEX model. Journal of King Saudi University – Science 2021;33(3):101394. https://doi.org/10.1016/j.jksus.2021.101394.
Bahriz, H, Bouras, N. Etude de la Maladie du Bayoud, le Comportement Variétal du Palmier Dattier vis-à-vis du Fusarium oxysporum f. sp. albedinis dans la Vallée du M’Zab. African Review of Science, Technology and Development 2020;5(1):41-60.
Chibane, E, Essarioui A, Ouknin M, Boumezzourh A, Bouyanzer A, Majidi L. Antifungal activity of Asteriscus graveolens (Forssk.) Less essential oil against Fusarium oxysporum f. sp. albedinis, the causal agent of “Bayoud” disease on date palm. Mor J Chem. 2020;8(2):456-65. http://revues.imist.ma/?journal=morjchem&page=login
Benzohra IE, Megateli M, Elayachi BA, Zekraoui M, Djillali K, Bouafia A, Benouis S, Benaziza A and A Rekis. Integrated management of Bayoud disease on date palm (Phoenix dactylifera L.) caused by Fusarium oxysporum f. sp. albedinis in Algeria. Journal Algérien des Régions Arides. 2017;14:93-100. https://www.researchgate.net/publication/315047463
Boudeffeur S, Selection of some cultivars of date palm in south of Algeria resistant to bayoud disease. the first international conference of date palm integrated crop management of date palm and its impacts for producing clean and safety dates; Cairo, Giza, Egypt; 2007 Sept 2-4.
Oihabi A. Technical report: Date palm genetic resources in North Africa. In:Proc. of the Date Palm International Symposium. Namibia, 22-25 February. 2000:333-335.
Moussouni S, Pintaud JC, Vigouroux Y, Bouguedoura N. Diversity of Algerian oases date palm (Phoenix dactylifera L., Arecaceae): Heterozygote excess and cryptic structure suggest farmer management had a major impact on diversity. PLOS ONE. 2017;12(4):0175232. https://doi.org/10.1371/journal.pone.0175232
Lemine FMM, Samb A, ZeinelAbidine OB, Mohamd Ahmed MVO, Taleb-khyar OD, Boukhari, AO. Assessment of physicochemical diversity in fruit of Mauritanian date palm (Phoenix dactylifera L.) cultivars. Afr J Agric Res. 2014;9:2167-76.
Ouafi S, Bounaga N, Lebreton PH, Bouguedoura N. Contribution à l’étude des hétérosides flavoniques du palmier dattier. Recherche de marqueurs des cultivars Algériens. Revue des régions Arides. 2008;2:379-85.
Abdulla M, Gamal O. Investigation on molecular phylogeny of some date palm (Phoenix dactylifera L.) cultivars by protein, RAPD and ISSR markers in Saudi Arabia. Aust J Crop Sci. 2010;4:23–28.
Khouane AC, Akkak A, Benbouza H. Molecular identification of Date palm (Phoenix dactylifera L.) "Deglet noor” pollinator through analysis of genetic diversity of Algerian male and female ecotypes using SSRs markers. Scientia Horticulturae. 2020;274:109668 https://doi.org/10.1016/j.scienta.2020.109668
Soumaya RC, Ghada B, Sonia DD, Salwa ZA, Mokhtar TJAJoB. Molecular research on the genetic diversity of Tunisian date palm (Phoenix dactylifera L.) using the random amplified microsatellite polymorphism (RAMPO) and amplified fragment length polymorphism (AFLP) methods. 2011;10:10352-65. https://doi.org/10.5897/AJB10.2242
Akkak A, Scariot V, Torello Marinoni D, Boccacci P, Beltramo C, Botta R. Development and evaluation of microsatrllite markers in Phoenix dactylifera L. and their transferability to other Phoenix species. Biol Plant. 2009;53(1):164-66.
Billotte N, Marseillac N, Brottier P, Noyer JL, Jacque moud-Collet JP, Moreau C. Nuclear microsatellite markers for the date palm (Phoenix dactylifera L.): characterization and utility across the genus Phoenix and in their palm genera. Molecular Ecology Notes. 2004;4:256-58. https://doi.org/10.1111/j.1471-8286.2004.00634.x
Nemati Z, Zeinalabedini M, Majidian P, Eftekharian Jahromi A, Kiani D. Phylogenetic relationships among Iranian and Spanish date palms (Phoenix dactylifera L.) revealed by microsatellite markers. The Journal of Horticultural Science and Biotechnology. 2014;89(2):115-20. https://doi.org/10.1080/14620316.2014.11513056
Ould safi M, Makhlouf L, Nedjahi A, Tolba K. Introduction de certaines espèces Forestières dans la région d’Adrar. La forêt Algérienne. 2015; N°10.
StatSoft, Inc. Electronic Statistics Textbook. Tulsa. 2012; OK: StatSoft. WEB: http://www.statsoft.com/textbook/ (references liste).
Peakall R, Smouse, PE GENALEX 6. Genetic Analysis in Excel, Population genetic software for teaching and research. Molecular Ecology Notes. 2006;6:288–95.
Kalinowski ST, Taper ML, Marshall TC. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology. 2007;6:1099-06. https://doi.org/10.1111/j.1365-294X.2007.03089.x
Marshall TC, CERVUS, 3,0, Cervus is a computer program for assignment of parents to their offspring using genetic markers, Cervus, a Windows package for parentage analysis using likelihood approach, CERVUS was written by Tristan Marshall 2006; http://www,fieldgenetics,com (Eri?imtarihi: 02,07,2008).
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6, 0, Molecular Biology and Evolution. 2013;30:2725-29. https://doi.org/10.1093/molbev/mst197
Nei M. Genetic distance between populations. Am Nat. 1972;160:283-92. http://dx.doi.org/10.1086/282771
Wright S. Evolution in Mendelian populations. Genetics. 1931;16:97–159.
Weir BS, Cockerham CC. Estimating F-statistics for the analysis of population structure, Evolution. 1984;38:1358–70.
Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX. POPGENE: The User-Friendly Shareware for Population Genetic Analysis, Edmonton, AB, Canada: University of Alberta; 1997.
Bedjaoui H, Benbouza H. Assessment of phenotypic diversity of local Algerian date palm (Phoenix dactylifera L.) cultivars. Journal of the Saudi Society of Agricultural Sciences. 2018; 19(1):65-75. https://doi.org/10.1016/j.jssas.2018.06.002
Amari I. Etude de la variabilité génétique des cultivars de palmier dattier (Phoenix dactylifera L.) et de leur agent causal Fusarium oxysporum f. sp. albedinis (Killian et Maire) Gordon dans les régions du Touat, Tidikelt et Gourara. Mémoire Master II; ENSA Alger; 2017.
Hamza H, BenAbderrahim MA, Elbekkay M, Ferdaous G, Triki T, Ferchichi A. Investigation of genetic variation in Tunisian date palm (Phoenix dactylifera L.) cultivars using ISSR marker systems and their relation with fruit characteristics. Turk J Biol. 2012;36:449-58. https://doi.org/10.3906/biy-1107-12
Bodian A, El Houmazi MA, Ndoye-Ndir K, Hasnaoui A, Nachtigall M. Genetic diversity analysis of date palm (Phoenix dactylifera L.) cultivars from Figuig oasis (Morocco) using SSR markers. International Journal of Science and Advanced Technology. 2012;2:96-104.
Zehdi S, Sakka H, Rhouma A, Ould Mohamed Salem A, Marrakchi M, Trifi M. Analysis of Tunisian date palm germplasm using simple sequence repeat primers. African journal of Biotechnology. 2004;3:215-19. https://doi.org/10.5897/AJB2004.000-2040
Hamza H, Elbekkay M, Benabderrahim MA, Ferchichi A. Molecular and morphological analyses of date palm (Phoenix dactylifera L.) subpopulations in southern Tunisia. Arid and oases cropping laboratory. Arid area institute. Medenine. Span J Agric Res Des. 2011;9(2):484-93. https://doi.org/10.5424/sjar/20110902-271-10
Elshibli S, Korpolainen H. Microsatelitte markers reveal diversity high genetic in date palm germplasm from Sudan. Genetica. 2008;134:251-60. https://doi.org/10.1007/s10709-007-9232-8
Bennaceur M, Lanaud C, Chevalier MH, Bounaga N. Genetic diversity of date palm (Phoenix dactylifera L.) from Algeria revealed by enzyme markers. Plant breed. 1991;107:56-69. https://doi.org/10.1111/j.1439-0523.1991.tb00528.x
Salem AOM, Trifi M, Sakka H, Rhouma A, Marrakchi MJGR. Genetic inheritance analysis of four enzymes in date-palm (Phoenix dactylifera L.). Genetic Resources and Crop Evolution. 2001;48:361-68.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Said Boudeffeur, Abdelkhader Ameur Ameur, M M Aci, Hafida Khelafi , Abdelkader Laaboudi , M Khersi, Abdullah Makhzoum, L Khelifi
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).