Phenotypic and genetic characterization of date palm cultivars resistant to bayoud disease

Authors

  • Said Boudeffeur Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria https://orcid.org/0000-0002-3226-5578
  • Abdelkhader Ameur Ameur Department of Agronomical sciences, University of Abou Bekr Belkaid, Tlemcen 13000, Algeria https://orcid.org/0000-0002-7450-4311
  • M M Aci Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria
  • Hafida Khelafi Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria https://orcid.org/0000-0002-9848-8424
  • Abdelkader Laaboudi Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria https://orcid.org/0000-0003-3697-028X
  • M Khersi Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria https://orcid.org/0000-0002-9182-7235
  • Abdullah Makhzoum Department of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, Palapye, Botswana
  • L Khelifi Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria

DOI:

https://doi.org/10.14719/pst.2021.8.4.1105

Keywords:

Algeria, Taqerbucht cultivars, Date morphological traits, SSR markers, Sequence comparisons

Abstract

Taqerbucht cultivars of date palm are well known by their natural resistance against devastating fungus Bayoud disease. In order to know, if these accessions have the same genetic and morphological profile or each of them constitutes a separate cultivar, we carried out a morphological and molecular characterization and we compared four Taqerbucht (Tq.) date palm cultivars from the southwestern region of Algeria: Tq.hamra cultivar (red fruits), Tq. safra cultivar (yellow fruits), Tq.beïda (white fruits) and Tq.kahla cultivar (black fruits). Seventy one phenotypic characteristics, including 33 quantitative and 38 qualitative traits, have been selected for comparison. Principal component analysis (PCA) and multi-component clustering were used to analyze and compare the data. The results suggest that the four cultivars can be classified into distinct groups. One group contains one cultivar, the Tq.kahla and another group contains the three other cultivars (Tq.safra, Tq.beïda and Tq. hamra). Based on phylogenetic analyses and sequence comparisons, the cultivar Tq. kahla seems to be divergent from the cultivar Tq.hamra, whereas the two cultivars Tq.Safra and Tq.beïda are close to each other. Using 16 Simple Sequence Repeat (SSR) genetic markers to analyze genetic diversity among the cultivars, we found that 13 markers were detectable in 31 allele's loci, and the number of alleles per locus varied from 1–4 with an average of 2.38 alleles per locus. Expected heterozygosity (He) values ranged from 0.375–0.500 and observed heterozygosity (Ho) values from 0.750–1.000.

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Author Biographies

Said Boudeffeur, Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria

Researcher at the National Institute of Agronomic Research of Algeria, specialized in phytopathology and plant improvement.

Abdelkhader Ameur Ameur, Department of Agronomical sciences, University of Abou Bekr Belkaid, Tlemcen 13000, Algeria

He is a teacher rearcher at the university of Tlemcen, Algeria and is an expert in animal genetics and breeding. His work is based on study of structure and diversity of animal populations using markers genetic such as SSR, MtDNA, SNP, and also some bioinformatics tools NGS.

M M Aci, Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria

He teaches at Mediterranean University of Reggio Calabria in Italy. His domain is Phytogenetic resources, agrobiodiversity, sustainable agriculture, molecular markers, etc.

Hafida Khelafi , Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria

He a researcher in the field of date palm. He has several contributions in the field of phytopathology, molecular and in vitro culture.

Abdelkader Laaboudi , Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria

He is a researcher at Algerian institute for researcher in agronomy. The research field is irrigation, bioclimatology and statistical analysis.

M Khersi, Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria

He an agronomic technician and laboratory technician. He is a specialist in plant biometrics.

Abdullah Makhzoum, Department of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, Palapye, Botswana

Associate Professor in plant biotechnology focusing on all plant improvments and plant biotechnolgy applications such as medicinal plants, plant molecular pharming, phytoremediation and plant nanopartciles.

L Khelifi , Laboratoire des Ressources génétiques et Biotechnologies, National Higher School of Agronomy (ENSA: ES1603), El-Harrach Algiers, Algeria

Doctorat d’Etat en Sciences de la nature et de la vie.

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Published

20-08-2021 — Updated on 01-10-2021

How to Cite

1.
Boudeffeur S, Ameur Ameur A, Aci MM, Khelafi H, Laaboudi A, Khersi M, Makhzoum A, Khelifi L. Phenotypic and genetic characterization of date palm cultivars resistant to bayoud disease. Plant Sci. Today [Internet]. 2021 Oct. 1 [cited 2024 Nov. 4];8(4):804–814. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1105

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