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Research Articles

Vol. 12 No. sp4 (2025): Recent Advances in Agriculture by Young Minds - III

Influence of different organic acids on enzymatic activity and postharvest quality in strawberry

DOI
https://doi.org/10.14719/pst.11490
Submitted
27 August 2025
Published
31-10-2025

Abstract

Strawberries are highly perishable fruits that suffer rapid quality loss during postharvest storage due to water loss, softening and microbial decay. Preserving their freshness, texture and nutritional value is essential to reduce postharvest losses and extend shelf life. This study evaluated the effectiveness of organic acids, such as ascorbic acid (AA), salicylic acid (SA) and oxalic acid (OA), at concentrations of 1, 2 and 3 mM in extending postharvest life. The study focused on preserving the quality of ‘Winter Dawn’ strawberries during 15 days of cold storage (5 ± 1 °C; 90-95 % relative humidity (RH)). The findings revealed that applying SA at a 3 mM concentration significantly reduced
physiological weight loss and decay. This treatment also minimized fruit softening by inhibiting cell wall-degrading enzymes, such as polygalacturonase and pectin methyl-esterase. Furthermore, SA at 3 mM enhanced the activity of reactive oxygen species (ROS) scavenging enzymes, including peroxidase and superoxide dismutase (SOD). Moreover, it preserved fruit quality by maintaining higher levels of titratable acidity, total phenolics and antioxidant activity. Additionally, strawberries treated with 3 mM SA retained stable sensory attributes, including color, taste, glossiness and overall acceptability, throughout storage. Therefore, SA at 3 mM is a promising, natural postharvest treatment for maintaining the quality and extending the shelf life of strawberries during cold storage.

References

  1. 1. Annual Report. National Horticulture Board, Director of Horticulture, Government of India 2023. Available from: https://www.nhb.gov.in
  2. 2. Seeram NP, Adams LS, Zhang Y, Lee R, Scheuller HS, Heber D. Blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. J Agric Food Chem. 2006;54:9329-39. https://doi.org/10.1021/jf061750g
  3. 3. Gol NP, Patel PR, Rao TVR. Improvement of quality and shelf-life of strawberries with edible coatings enriched with chitosan. Postharvest Biol Technol. 2013;85:185-95. https://doi.org/10.1016/j.postharvbio.2013.06.008
  4. 4. Caleb J, Ilte K, Herppich WB, Geyer M, Mahajan PV. Impacts of minimal processing and hot water dipping of ‘Sonata’ strawberries on volatiles emitted during storage. Sci Hortic. 2019;243:385-91. https://doi.org/10.1016/j.scienta.2018.08.054
  5. 5. Almenar E, Munoz PH, Lagaron JM, Catala R, Gavara R. Controlled atmosphere storage of wild strawberry fruit (Fragaria vesca L.). J Agric Food Chem. 2006;54:86-91. https://doi.org/10.1021/jf0517492
  6. 6. Khreba AH, Hassan AH, Emam MS, Atala SA. Effect of some pre and postharvest treatments on quality and storability of strawberry fruits. J Amer Sci. 2014;10:239-48. http://doi.org/10.36632/mejar/2023.12.4.46
  7. 7. Asghari M, Aghdam MS. Impact of salicylic acid on post-harvest physiology of horticultural crops. Trend Food Sci Technol. 2010;21:502-9. https://doi.org/10.1016/j.tifs.2010.07.009
  8. 8. Zhang H, Jiang S, Lin H, Zhang X, Ge L, Xu Z. Enhancement of biocontrol efficacy of Rhodotorula glutinis by salicylic acid against gray mold spoilage of strawberries. Int J Food Microbiol. 2010;141:122-5. https://doi.org/10.1016/j.ijfoodmicro.2010.04.022
  9. 9. Beckers GJM, Spoel SH. Fine-tuning plant defense signaling: salicylate versus jasmonate. Plant Biol. 2006;8:1-10. https://doi.org/10.1055/s-2005-872705
  10. 10. Lolaei A, Kaviani B, Rezaei MA, Raad MK, Mohammadipour R. Effect of pre- and postharvest treatment of salicylic acid on ripening of fruit and overall quality of strawberry (Fragaria × ananassa Duch cv. Camarosa) fruit. Sci Hortic. 2012;32:4680-4.
  11. 11. Zhang Y, Chen K, Zhang S, Ferguson I. The role of salicylic acid in postharvest ripening of kiwi fruit. Postharvest Biol Technol. 2003;28:67-74. https://doi.org/10.1016/S0925-5214(02)00172-2
  12. 12. Wang L, Chen S, Kong W, Li S, Archbold DD. Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during cold storage. Postharvest Biol Technol. 2006;41:244-51. https://doi.org/10.1016/j.postharvbio.2006.04.010
  13. 13. Ranjbaran E, Sarikhani H, Bakhshi D, Mehrdad P. Investigation of salicylic acid application to reduce postharvest losses in stored ‘Bidaneh Ghermez’ table grapes. Int J Fruit Sci. 2011;11:430-9. https://doi.org/10.1080/15538362.2011.630591
  14. 14. Xu X, Tian S. Salicylic acid alleviated pathogen-induced oxidative stress in harvested sweet cherry fruit. Postharvest Biol Technol. 2008;49:379-85. https://doi.org/10.1016/j.postharvbio.2008.02.003
  15. 15. Srivastava MK, Dwivedi UN. Delayed ripening of banana fruit by salicylic acid. Plant Sci. 2000;158:87-96. https://doi.org/10.1016/S0168-9452(00)00304-6
  16. 16. Babalar M, Asghari M, Talaei A, Khosroshahi A. Effect of pre- and postharvest salicylic acid treatment on ethylene production, fungal decay and overall quality of Selva strawberry fruit. Food Chem. 2007;105:449-53. https://doi.org/10.1016/j.foodchem.2007.03.021
  17. 17. Ren YY, Sun PP, Wang XX, Zhu ZY. Degradation of cell wall polysaccharides and change of related enzyme activities with fruit softening in Annona squamosa during storage. Postharvest Biol Technol. 2020;166:111-203. https://doi.org/10.1016/j.postharvbio.2020.111203
  18. 18. Hangermann AE, Austin PJ. Continuous spectrophotometric assay for plant pectin methyl-esterase. J Agric Food Chem. 1986;34:440-4. https://doi.org/10.1021/JF00069A015
  19. 19. Kaur A, Grewal SK, Singh R, Kaur J. Defense system in chickpea genotypes differing in tolerance to Helicoverpa armigera infestation. Indian J Plant Physiol. 2017;22:324-34. https://doi.org/10.1007/s40502-017-0310-3
  20. 20. Adhikary T, Gill PPS, Jawandha SK, Bhardwaj RD, Anurag RK. Efficacy of postharvest sodium nitroprusside application to extend storability by regulating physico-chemical quality of pear fruit. Food Chem. 2021;346:128-34. https://doi.org/10.1016/j.foodchem.2020.128934
  21. 21. Barnes M, Patchett B. Cell wall degrading enzymes and the softening of senescent strawberry fruit. J Food Sci. 2006;41:1392-5. https://doi.org/10.1111/j.1365-2621.1976.tb01179.x
  22. 22. Saleem MS, Anjum MA, Naz S, Ali S, Hussain S, Azam M, Ejaz S. Incorporation of ascorbic acid in chitosan-based edible coating improves postharvest quality and storability of strawberry fruits. Int J Biol Macromol. 2021;189:160-9. https://doi.org/10.1016/j.ijbiomac.2021.08.051
  23. 23. Geransayeh M, Sepahvand S, Abdossi V. Extending postharvest longevity and improving quality of strawberry (Fragaria × ananassa Duch Cv. ‘Gaviota’) fruit by postharvest salicylic acid treatment. J Agric Stud. 2015;3:17-9. http://doi.org/10.5296/jas.v3i2.7274
  24. 24. Alali AA, Awad MA, Al-Qurashi AD, Mohamed SA. Postharvest gum Arabic and salicylic acid dipping affect quality and biochemical changes of ‘Grand Nain’ bananas during shelf life. Sci Hortic. 2018;237:51-8. https://doi.org/10.1016/j.scienta.2018.03.061
  25. 25. Abu-Goukh AA, Bashir HA. Changes in pectic enzymes and cellulase activity during guava fruit ripening. Food Chem. 2003;83:213-8. https://doi.org/10.1016/S0308-8146(03)00067-0
  26. 26. Brummell DA, Harpster MH. Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol Biol. 2001;47:31-40. https://doi.org/10.1023/A:1010656104304
  27. 27. Singh P, Dwivedi UN. Purification and characterization of multiple forms of polygalacturonase from mango (Mangifera indica cv. Dashehari) fruit. Food Chem. 2008;111:345-9. https://doi.org/10.1016/j.foodchem.2008.03.072
  28. 28. Li Y, Hou He H, Kelimu Y, Wu FA, Zhao Y, Shi L, Zhu X. Salicylic acid treatment delays apricot (Prunus armeniaca L.) fruit softening by inhibiting ethylene biosynthesis and cell wall degradation. Sci Hortic. 2022;300:11061. https://doi.org/10.2139/ssrn.4017119
  29. 29. Shi Z, Yang H, Jiao J, Wang F, Lu Y, Deng J. Effects of graft copolymer of chitosan and salicylic acid on reducing rot of postharvest fruit and retarding cell wall degradation in grapefruit during storage. Food Chem. 2019;283:92-100. https://doi.org/10.1016/j.foodchem.2018.12.078
  30. 30. Zhang Y, Zhang M, Yang H. Postharvest chitosan-g-salicylic acid application alleviates chilling injury and preserves cucumber fruit quality during cold storage. Food Chem. 2015;174:558-63. http://doi.org/10.1016/j.foodchem.2014.11.106
  31. 31. Wang H, Li Y, Wassie M, Huo L, Shi H. Salicylic acid spray delays sand pear fruit senescence during room temperature shelf life by regulating antioxidant capacity and senescence-related genes. Plants (Basel). 2024;15:13:848. https://doi.org/10.3390/plants13060848
  32. 32. Campos-Vargas R, Saltveit ME. Involvement of putative chemical wound signals in the induction of phenolic metabolism in wounded lettuce. Physiol Plant. 2002;114:73-84. https://doi.org/10.1034/j.1399-3054.2002.1140111.x
  33. 33. Shahzad S, Ahmad S, Anwar R, Ahmad R. Pre-storage application of calcium chloride and salicylic acid maintain the quality and extend the shelf life of strawberry. Pak J Agric Sci. 2020;57:102-9. https://doi.org/10.21162/PAKJAS/20.8953
  34. 34. Kim Y, Kim S, Shim IS. Exogenous salicylic acid alleviates salt-stress damage in cucumber under moderate nitrogen conditions by controlling endogenous salicylic acid levels. Hortic Environ Biotechnol. 2017;58:247-53. https://doi.org/10.3390/ijms26094447
  35. 35. Racchi M. Antioxidant defences in plants with attention to Prunus and Citrus spp. Antioxid. 2013;2:340-69. https://doi.org/10.3390/antiox2040340
  36. 36. Ayub M, Ayub Q, Khan M, Khan A, Muhammad A, Sohail H, et al. Prestorage application of ascorbic acid and salicylic acid to preserve quality of peach fruits during cold storage. J Pure Appl Agri. 2022;7(4). https://doi.org/10.36632/jpaa/903
  37. 37. Cao S, Hu Z, Zheng Y, Lu B. Synergistic effect of heat treatment and salicylic acid on alleviating internal browning in cold-stored peach fruit. Postharvest Biol Technol. 2010;58:93-7. https://doi.org/10.1016/j.postharvbio.2010.05.010

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