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Plant Science Today (PST)

Research Articles

Early Access

Standardization of an efficient protocol for rapid in vitro micropropagation of hydrangea (Hydrangea macrophylla Thunb. Ser)

DOI
https://doi.org/10.14719/pst.11685
Submitted
8 September 2025
Published
18-11-2025
Versions

Abstract

Micropropagation is a valuable tool for producing large numbers of clonal plants via tissue culture through morphogenesis induction. This work aimed to obtain an efficient protocol for in vitro regeneration and micropropagation of Hydrangea macrophylla. Organogenesis in H. macrophylla was achieved on Murashige and Skoog (MS) medium fortified with 2.5 ppm 6-benzylamino purine (BAP), where the minimum number of days for shoot initiation (17.33), maximum number of shoots per explant (2.30), highest number of leaves per explant (8.37) and highest fresh weight (6.26 g) was recorded. Multiple shoot production with combination of MS with 3.5 ppm BAP gave the maximum number of shoots initiated per explant (3.82), maximum number of leaves per explant (16.26) during first sub culturing and during second sub culturing same media combination gave the maximum number of shoots per explant (4.32), maximum number of leaves per explant (21.13) and shoot length (3.48 cm) was recorded. Regenerated shoots rooted best on MS medium fortified with 0.5 ppm naphthaleneacetic acid (NAA), which produced the maximum number of roots (4.54), maximum rooting percent (92.53 %) and early root initiation in (16.24 days). The longest root length (3.91 cm) was recorded on MS medium with 1.0 ppm NAA.

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