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Research Articles

Vol. 13 No. 3 (2026)

Phytochemical screening and antioxidant activity of ethanolic extracts of Homalomena cochinchinensis Engl.

DOI
https://doi.org/10.14719/pst.12274
Submitted
15 October 2025
Published
02-07-2026 — Updated on 09-07-2026
Versions

Abstract

This study aimed to investigate the phytochemical composition and antioxidant activity of ethanolic extracts from the rhizome and root of Homalomena cochinchinensis Engl., a rare plant species from Vietnam. Phytochemical screening was conducted to identify major bioactive compounds and antioxidant activity was evaluated using the DPPH radical-scavenging assay. Both extracts were found to contain phenolics, tannins, terpenoids, steroids, flavonoids, coumarins, alkaloids and saponins. Quantitative analysis showed that the rhizome extract exhibited higher contents of triterpenes (3.77 mg Oleuropein aglycone equivalent (OAE)/g dry weight (DW)), total polyphenols (29.85 mg Quercetin acid equivalent (GAE)/g DW) and flavonoids (20.94 mg Quercetin equivalents (QE)/g DW) compared to the root extract (0.86 mg OAE/g DW, 8.73 mg GAE/g DW and 10.77 mg QE/g DW respectively). The rhizome and root extracts also demonstrated antioxidant activity with IC₅₀ values of 6.32 and 6.54 mg/mL respectively. These findings indicate that H. cochinchinensis, particularly its rhizome, is a promising natural source of antioxidant compounds.

References

  1. 1. Hay A. Revision of Homalomena (Araceae-Homalomeneae) in New Guinea, the Bismarck Archipelago and Solomon Islands. Blumea. 1999;44:41–71.
  2. 2. Kiaw NK, Othman S, Boyce BC, Yeng WS. Studies on Homalomeneae (Araceae) of Borneo VIII: Delimitation of additional informal suprageneric taxa for Sundaic Homalomena. Webbia. 2011;66(1):21–8. https://doi.org/10.1080/00837792.2011.10670880
  3. 3. Van HT. Building phylogenetic trees for the Araceae in southern Vietnam based on morphological and molecular markers [dissertation]. Vietnam: Graduate University of Science and Technology, Vietnam Academy of Science and Technology; 2017.
  4. 4. Sung TV, Steffan B, Steglich W, Klebe G, Adam G. Sesquiterpenoids from the roots of Homalomena aromatica. Phytochemistry. 1992;31(10):3515–20. https://doi.org/10.1016/0031-9422(92)83719-F
  5. 5. Schultes RE. Psychoactive plants in need of chemical and pharmacological study. Proc Indian Acad Sci. 1984;93(3):281–304. https://doi.org/10.1007/BF03053083
  6. 6. Habibullah Khan M, Singh Yadava P. Herbal remedies of asthma in Thoubal District of Manipur in North East India. Indian J Nat Prod Resour. 2009;1(1):80–4.
  7. 7. Dam SM, Van HT. Chemical profiles and biological activities of essential oils of Arisaema and Homalomena species (Araceae): A review. J Phytol. 2022;14:41–9. https://doi.org/10.25081/jp.2022.v14.7444
  8. 8. Le TT, Le LS, Nguyen VD, Ho XAV, Nguyen QM, Tran TM, et al. In vitro antioxidant activity and content of bioactive compounds from Homalomena occulta. Hue Univ J Sci Nat Sci. 2024;133(1B):79–87. https://doi.org/10.26459/hueunijns.v133i1B.7256
  9. 9. Van HT, Le NT, Huynh NTA, Vo HS, Le TT, Van Chu H, et al. Chemical composition and antibacterial activities of essential oils from rhizomes and aerial parts of Homalomena cochinchinensis (Araceae). Nat Prod Res. 2022;36(12):3129–32. https://doi.org/10.1080/14786419.2021.1939333
  10. 10. Nguyen LTK, Doan TQ, Nguyen PQD, Nguyen CBH, Tran LTT, Tran TVA, et al. Phytochemical composition and bioactivities of essential oils from rhizomes of Homalomena pendula and Homalomena cochinchinensis. Nat Prod Commun. 2023;18(5). https://doi.org/10.1177/1934578X231175263
  11. 11. Van HT, Le NT, Nguyen DL, Tran GB, Huynh NTA, Vo HS, et al. Chemical profile and antibacterial activity of acetone extract of Homalomena cochinchinensis Engl. (Araceae). Plant Sci Today. 2021;8(1):58–65. https://doi.org/10.14719/pst.2021.8.1.971
  12. 12. Nguyen LTK, Nguyen PQD, Doan NAT, Nguyen CBH, Doan TQ, Tran LTT, et al. Volatile components and biological activities of n-hexane extract from rhizomes of Homalomena cochinchinensis. Nat Prod Commun. 2023;18(4). https://doi.org/10.1177/1934578X231168481
  13. 13. Zeng LB, Zhang ZR, Luo ZH, Zhu JX. Antioxidant activity and chemical constituents of essential oil and extracts of Rhizoma Homalomenae. Food Chem. 2011;125(2):456–63. https://doi.org/10.1016/j.foodchem.2010.09.029
  14. 14. Ali S, Sayem SAJS, Habibullah, Quah Y, Lee EB, Birhanu BT, et al. Investigation of potential antioxidant, thrombolytic and neuropharmacological activities of Homalomena aromatica leaves using experimental and in silico approaches. Molecules. 2021;26(4):975. https://doi.org/10.3390/molecules26040975
  15. 15. Vo T, Nguyen T, Nguyen T, Tran H, Nguyen D. Investigation of total phenolic content, total flavonoids, antioxidant activity and antibacterial activity of ethanol and methanol extracts of leaves and rhizomes of Imperata cylindrica. Can Tho Univ J Sci. 2017;52:16–22.
  16. 16. Llauradó G, Morris HJ, Lebeque Y, Gutiérrez A, Fontaine R, Bermúdez RC, et al. Phytochemical screening and effects on cell-mediated immune response of Pleurotus fruiting bodies powder. Food Agric Immunol. 2013;24(3):295–304. https://doi.org/10.1080/09540105.2012.686988
  17. 17. Shaikh JR, Patil M. Qualitative tests for preliminary phytochemical screening: An overview. Int J Chem Stud. 2020;8(2):603–8. https://doi.org/10.22271/chemi.2020.v8.i2i.8834
  18. 18. Nguyen D, Vo N, Nguyen V, Phan T, Nguyen T, Huynh N. Investigation of biochemical properties and antibacterial properties of extracts from Colocasia esculenta. J Agric Sci Technol Hue Univ Agric For. 2017;1(2):265–74.
  19. 19. Bodi D, Ronczka S, Gottschalk C, Behr N, Skibba A, Wagner M, et al. Determination of pyrrolizidine alkaloids in tea, herbal drugs and honey. Food Addit Contam Part A. 2014;31(11):1886–95. https://doi.org/10.1080/19440049.2014.964337
  20. 20. Deka AC, Sarma I, Dey S, Sarma TC. Antimicrobial properties and phytochemical screening of some wild macrofungi of Rani-Garbhanga Reserve Forest area of Assam, India. Adv Appl Sci Res. 2017;8(3):17–22.
  21. 21. Kadarani DK, Setyadjit S, Seno DSH, Sukasih E. Total phenol and antioxidant from seed and peel of ripe and unripe Indonesian sugar apple (Annona squamosa L.) extracted with various solvents. IOSR J Pharm. 2015;5(10):20–5.
  22. 22. Chen Y, Xie MY, Gong XF. Microwave-assisted extraction used for the isolation of total triterpenoid saponins from Ganoderma atrum. J Food Eng. 2007;81(1):162–70. https://doi.org/10.1016/j.jfoodeng.2006.10.018
  23. 23. Sen S, De B, Devanna N, Chakraborty R. Total phenolic, total flavonoid content and antioxidant capacity of the leaves of Meyna spinosa Roxb., an Indian medicinal plant. Chin J Nat Med. 2013;11(2):149–57. https://doi.org/10.1016/S1875-5364(13)60042-4
  24. 24. Li S, Li SK, Gan RY, Song FL, Kuang L, Li HB. Antioxidant capacities and total phenolic contents of infusions from 223 medicinal plants. Ind Crops Prod. 2013;51:289–98. https://doi.org/10.1016/j.indcrop.2013.09.017
  25. 25. Kumari M, Kalita R, Dey BK, Choudhury A. Evaluation of preliminary phytochemical and in vitro antioxidant activity of Homalomena aromatica (tuber). J Appl Pharmacogn Phytochem. 2021;1(1):15–21.
  26. 26. Roy SJ, Baruah PS, Lahkar L, Gurung L, Saikia D, Tanti B. Phytochemical analysis and antioxidant activities of Homalomena aromatica Schott. J Pharmacogn Phytochem. 2019;8(1):1379–85.

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