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Plant Science Today (PST)

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Early Access

Evaluation of salt tolerance in cotton (Gossypium hirsutum L.) seedlings using the multitrait genotype-ideotype distance index (MGIDI)

DOI
https://doi.org/10.14719/pst.12364
Submitted
20 October 2025
Published
07-01-2026

Abstract

Cotton is a vital agricultural crop and a primary source of natural fibres, linters and oil. However, soil salinity poses a major abiotic threat to crop productivity worldwide, particularly in arid and semi-arid regions. Although cotton is considered moderately salt-tolerant, its early growth stages are highly sensitive to stress. This study evaluated the morphological response of 28 cotton cultivars to controlled salt stress conditions. Results revealed a clear, dose-dependent reduction in seedling growth under increasing salinity levels. Analysis of variance (ANOVA) confirmed a significant cultivar × treatment interaction (PC × PT), highlighting substantial genetic variability and contrasting responses of cultivars to salinity. Pearson correlation analysis indicated stronger relationships between linear growth traits and biomass accumulation under saline conditions, suggesting a coordinated adaptive modulation of growth mechanisms. Principal component analysis (PCA) confirmed the dominant effect of salinity on phenotypic variability and distinguished distinct adaptive growth strategies among cultivars. The multitrait genotype-ideotype distance index (MGIDI) effectively identified the cultivars Bukhoro-14 (C5), C-4727 (C7), Kelajak (C14) and Nasaf (C19) as genotypes with enhanced salt tolerance at specific levels of induced salt stress. These genotypes represent valuable genetic resources for breeding programs aimed at developing salt-tolerant cotton cultivars suited to arid environments.   

References

  1. 1. Khan MA, Wahid A, Ahmad M, Tahir MT, Ahmed M, Ahmad S, et al. World cotton production and consumption: An overview. In: Ahmad S, Hasanuzzaman M, editors. Cotton production and uses: agronomy, crop protection and postharvest technologies. Singapore: Springer Singapore; 2020. p. 1-7. https://doi.org/10.1007/978-981-15-1472-2_1
  2. 2. Maryum Z, Luqman T, Nadeem S, Khan SMUD, Wang B, Ditta A, et al. An overview of salinity stress, mechanism of salinity tolerance and strategies for its management in cotton. Front Plant Sci. 2022; 13:907937. https://doi.org/10.3389/fpls.2022.907937
  3. 3. Ijaz B, Zhao N, Kong J, Hua J. Fiber quality improvement in upland cotton (Gossypium hirsutum L.): Quantitative trait loci mapping and marker assisted selection application. Front Plant Sci. 2019;10:1585. https://doi.org/10.3389/fpls.2019.01585
  4. 4. Singh A. Soil salinity: A global threat to sustainable development. Soil Use Manag. 2022;38:39-67. https://doi.org/10.1111/sum.12772
  5. 5. Hopmans JW, Qureshi AS, Kisekka I, Munns R, Grattan SR, Rengasamy P, et al. Critical knowledge gaps and research priorities in global soil salinity. Adv Agron. 2021;169:1-191. https://doi.org/10.1016/bs.agron.2021.03.001
  6. 6. Chaudhary MT, Majeed S, Rana IA, Ali Z, Jia Y, Du X, et al. Impact of salinity stress on cotton and opportunities for improvement through conventional and biotechnological approaches. BMC Plant Biol. 2024;24:1-13. https://doi.org/10.1186/s12870-023-04558-4
  7. 7. Ahmed N, Chaudhry UK, Ali MA, Ahmad F, Sarfraz M, Hussain S. Salinity tolerance in cotton. In: Ahmad S, Hasanuzzaman M, editors. Cotton production and uses: agronomy, crop protection and postharvest technologies. Singapore: Springer Singapore; 2020. p. 367-91. https://doi.org/10.1007/978-981-15-1472-2_19
  8. 8. Zafar MM, Shakeel A, Haroon M, Manan A, Sahar A, Shoukat A, et al. Effects of salinity stress on some growth, physiological and biochemical parameters in cotton (Gossypium hirsutum L.) germplasm. J Nat Fibers. 2022;19:8854-86. https://doi.org/10.1080/15440478.2021.1975596
  9. 9. Munawar W, Hameed A, Khan MKR. Differential morphophysiological and biochemical responses of cotton genotypes under various salinity stress levels during early growth stage. Front Plant Sci. 2021;12:622309. https://doi.org/10.3389/fpls.2021.622309
  10. 10. Dong H, Kong X, Luo Z, Li W, Xin C. Unequal salt distribution in the root zone increases growth and yield of cotton. Eur J Agron. 2010;33:285-92. https://doi.org/10.1016/j.eja.2010.08.002
  11. 11. Gul N, Khan Z, Shani MY, Hafiza BS, Saeed A, Khan AI, et al. Identification of salt-resilient cotton genotypes using integrated morpho-physiological and biochemical markers at the seedling stage. Sci Rep. 2025;15:1-14. https://doi.org/10.1038/s41598-025-89582-0
  12. 12. Rodriguez-Uribe L, Higbie SM, Stewart JMD, Wilkins T, Lindemann W, Sengupta-Gopalan C, et al. Identification of salt responsive genes using comparative microarray analysis in upland cotton (Gossypium hirsutum L.). Plant Sci. 2011;180:461-69. https://doi.org/10.1016/j.plantsci.2010.10.009
  13. 13. Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9:671-75. https://doi.org/10.1038/nmeth.2089
  14. 14. Olivoto T, Lúcio ADC. metan: an R package for multi-environment trial analysis. Methods Ecol Evol. 2020;11:783-89. https://doi.org/10.1111/2041-210X.13384
  15. 15. Zeeshan M, Lu M, Sehar S, Holford P, Wu F. Comparison of biochemical, anatomical, morphological and physiological responses to salinity stress in wheat and barley genotypes deferring in salinity tolerance. Agronomy. 2020;10:127. https://doi.org/10.3390/agronomy10010127
  16. 16. Hasanuzzaman M, Raihan MRH, Masud AAC, Rahman K, Nowroz F, Rahman M, et al. Regulation of reactive oxygen species and antioxidant defense in plants under salinity. Int J Mol Sci. 2021;22:9326. https://doi.org/10.3390/ijms22179326
  17. 17. Li H, Testerink C, Zhang Y. How roots and shoots communicate through stressful times. Trends Plant Sci. 2021;26:940-52. https://doi.org/10.1016/j.tplants.2021.03.005
  18. 18. Zhang M, Pan M, Li H, Liu B, Qiao S, Ma CM, et al. Plant survival strategies under heterogeneous salt stress: remodeling of root architecture, ion dynamic balance and coordination of metabolic homeostasis. Plant Soil. 2025;1-40. https://doi.org/10.1007/s11104-025-07760-5
  19. 19. Acharya R, Gangopadhyay D, Bhattacharyya P, Ghosh A. Evaluation of salt-tolerant germplasm of mulberry (Morus L.) through in vitro and field experiments under different salinity stresses. Heliyon. 2024;10:e35868. https://doi.org/10.1016/j.heliyon.2024.e35868
  20. 20. Arshad I, Saleem M, Akhtar M, Shani MY, Farid G, Jarecki W, et al. Enhancing fruit retention and juice quality in 'Kinnow' (Citrus reticulata) through the combined foliar application of potassium, zinc and plant growth regulators. Horticulturae. 2024;10:1245. https://doi.org/10.3390/horticulturae10121245

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