Effect of Rhizophagus intraradices on growth and physiological performance of Finger Millet (Eleusine coracana L.) under drought stress
DOI:
https://doi.org/10.14719/pst.2021.8.4.1240Keywords:
Antioxidants, Drought, Finger millet, Mycorrhizal fungi, ROSAbstract
Under abiotic stress conditions, arbuscular mycorrhizal (AM) fungi help plants by improving nutrient and water uptake. Finger millet (Eleusine coracana L.) is an arid crop having soils with poor water holding capacity. Therefore, it is difficult for the plants to obtain water and mineral nutrients from such soil to sustain life. To understand the role of mycorrhizal symbiosis in water and mineral up-take from the soil, we studied the role of Rhizophagus intraradices colonization and its beneficial role for drought stress tolerance in finger millet seedlings. Under severe drought stress condition, AM inoculation led to the significant increase in plant growth (7 %), phosphorus and chlorophyll content (29 %). Also, under drought stress the level of osmolytes such as proline and soluble sugars were found to be increased in AM inoculated seedlings. Under water stress, the lipid peroxidation in leaves of mycorrhized seedlings was reduced by 29 %. The flavonoid content of roots in AM colonized seedlings was found 16 % higher compared to the control, whereas the leaves were accumulated more phenol. Compared to the control, ascorbate level was found to be 25 % higher in leaf tissue of AM inoculated seedlings. Moreover, glutathione (GSH) level was also increased in mycorrhiza inoculated seedlings with a maximum increment of 182 % under severe stress. The results demonstrated that AM provided drought tolerance to the finger millet seedlings through a stronger root system, greater photosynthetic efficiency, a more efficient antioxidant system and improved osmoregulation.
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Upadhyaya HD, Ramesh S, Sharma S, Singh SK, Varshney SK, Sarma ND, Ravishankar CR, Narasimhudu Y, Reddy VG, Sahrawat KL, Dhanalakshmi TN. Genetic diversity for grain nutrients contents in a core collection of finger millet (Eleusine coracana (L.) Gaertn.) germplasm. Field Crops Research. 2011;121(1):42-52. https://doi.org/10.1016/j.fcr.2010.11.017
Kumar A, Gaur VS, Goel A, Gupta AK. De novo assembly and characterization of developing spikes transcriptome of finger millet (Eleusine coracana): a minor crop having nutraceutical properties. Plant Molecular Biology Reporter. 2015;33(4):905-22. https://doi.org/10.1007/s11105-014-0802-5
Rosegrant MW, Cai X, Cline SA. World water and food to 2025: dealing with scarcity. Intl Food Policy Res Inst. 2002.
Kurepin LV, Ivanov AG, Zaman M, Pharis RP, Hurry V, Hüner NP. Interaction of glycine betaine and plant hormones: protection of the photosynthetic apparatus during abiotic stress. In: Photosynthesis: Structures, mechanisms and applications 2017;185-202. Springer, Cham. https://doi.org/10.1007/978-3-319-48873-8_9
Choudhury FK, Rivero RM, Blumwald E, Mittler R. Reactive oxygen species, abiotic stress and stress combination. The Plant Journal. 2017;90(5):856-67. https://doi.org/10.1111/tpj.13299
Noctor G, Mhamdi A, Foyer CH. The roles of reactive oxygen metabolism in drought: not so cut and dried. Plant Physiology. 2014;164(4):1636-48. https://doi.org/10.1104/pp.113.233478
Petrov V, Hille J, Mueller-Roeber B, Gechev TS. ROS-mediated abiotic stress-induced programmed cell death in plants. Frontiers in Plant Science. 2015;18(6):691-96. https://doi.org/10.3389/fpls.2015.00069
Ma D, Sun D, Wang C, Li Y, Guo T. Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress. Plant Physiology and Biochemistry. 2014;(1):80-60-66. https://doi.org/10.1016/j.plaphy.2014.03.024
Das K, Roychoudhury A. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science. 2014;(2):531-5313. https://doi.org/10.3389/fenvs.2014.00053
Augé RM, Toler HD, Saxton AM. Arbuscular mycorrhizal symbiosis alters stomatal conductance of host plants more under drought than under amply watered conditions: a meta-analysis. Mycorrhiza. 2015;25(1):13-24. https://doi.org/10.1007/s00572-014-0585-4
Dastogeer KM, Wylie SJ. Plant-fungi association: role of fungal endophytes in improving plant tolerance to water stress. In: Plant-microbe interactions in agro-ecological perspectives. 2017; pp.143-159. Springer, Singapore. https://doi.org/10.1007/978-981-10-5813-4_8
Miransari M. Contribution of arbuscular mycorrhizal symbiosis to plant growth under different types of soil stress. Plant Biology. 2010;12(4):563-69. https://doi.org/10.1111/j.1438-8677.2009.00308.x
Gonzalez-Dugo V, Durand JL, Gastal F. Water deficit and nitrogen nutrition of crops. A review. Agronomy for sustainable development. 2010;30(3):529-44. https://doi.org/10.1051/agro/2009059
Zou YN, Wang P, Liu CY, Ni QD, Zhang DJ, Wu QS. Mycorrhizal trifoliate orange has greater root adaptation of morphology and phytohormones in response to drought stress. Scientific Reports. 2017;7(1):1-10. https://doi.org/10.1038/srep41134
Karasawa T, Takebe M. Temporal or spatial arrangements of cover crops to promote arbuscular mycorrhizal colonization and P uptake of upland crops grown after nonmycorrhizal crops. Plant and Soil. 2012;353(1):355-66. https://doi.org/10.1007/s11104-011-1036-z
Ramakrishnan K, Bhuvaneswari G. Effect of inoculation of am fungi and beneficial microorganisms on growth and nutrient uptake of Eleusine coracana (L.) Gaertn. (Finger millet). International Letters of Natural Sciences. 2014;8(2):59-69. https://doi.org/10.18052/www.scipress.com/ILNS.13.59
Kamal R, Gusain Y.S, Sharma I.P, Sharma S. and Sharma A.K., 2015. Impact of arbuscular mycorrhizal fungus, Glomus intraradices, Streptomyces and Pseudomonas spp. strain on finger millet (Eleusine coracana L.) cv Korchara under water deficit condition. African Journal of Biotechnology. 2015; 14(48):3219-27. https://doi.org/10.5897/AJB2015.14479
Tisserant E, Malbreil M, Kuo A, Kohler A, Symeonidi A, Balestrini R, Charron P, Duensing N, dit Frey NF, Gianinazzi-Pearson V, Gilbert LB. Genome of an arbuscular mycorrhizal fungus provides insight into the oldest plant symbiosis. Proceedings of the National Academy of Sciences. 2013; 110(50):20117-22. https://doi.org/10.1073/pnas.1313452110
Colman EA. A laboratory procdure for determining the field capacity of soils. Soil Science. 1947;1;63(4):277-84. https://doi.org/10.1097/00010694-194704000-00003
Nouri E, Breuillin-Sessoms F, Feller U, Reinhardt D. Phosphorus and nitrogen regulate arbuscular mycorrhizal symbiosis in Petunia hybrida. PloS one. 2014;7;9(3):e90841. https://doi.org/10.1371/journal.pone.0090841
Giovannetti M, Mosse B. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist. 1980;(84):489-500. https://doi.org/10.1111/j.1469-8137.1980.tb04556.x
Hiscox JD, Israelstam GF. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany. 1979;57(12):1332-4. https://doi.org/10.1139/b79-163
Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant physiology. 1949;24(1):1. https://doi.org/10.1104/pp.24.1.1
Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant and Soil. 1973;39(1):205-7. https://doi.org/10.1007/BF00018060
Wang S, Zhu Y, Jiang H, Cao W. Positional differences in nitrogen and sugar concentrations of upper leaves relate to plant N status in rice under different N rates. Field Crops Research. 2006;96(2-3):224-34. https://doi.org/10.1016/j.fcr.2005.07.008
Li Y, Zhao H, Duan B, Korpelainen H, Li C. Effect of drought and ABA on growth, photosynthesis and antioxidant system of Cotinus coggygria seedlings under two different light conditions. Environmental and Experimental Botany. 2011; 1;71(1):107-13. https://doi.org/10.1016/j.envexpbot.2010.11.005
Junglee S, Urban L, Sallanon H, Lopez-Lauri F. Optimized assay for hydrogen peroxide determination in plant tissue using potassium iodide. American Journal of Analytical Chemistry. 2014;5(11):730-36. https://doi.org/10.4236/ajac.2014.511081
Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA)-general subjects. 1979;582(1):67-78. https://doi.org/10.1016/0304-4165(79)90289-7
Mukherjee SP, Choudhuri MA. Implications of water stress?induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiologia Plantarum. 1983;58(2):166-70. https://doi.org/10.1111/j.1399-3054.1983.tb04162.x
Bettaieb I, Hamrouni-Sellami I, Bourgou S, Limam F, Marzouk B. Drought effects on polyphenol composition and antioxidant activities in aerial parts of Salvia officinalis L. Acta Physiologiae Plantarum. 2011;33(4):1103-11. https://doi.org/10.1007/s11738-010-0638-z
Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture. 1977;28(1):49-55.
Verma JP. Data Analysis in Management with SPSS Software. Springer Science and Business Media. 2012 Dec 13. https://doi.org/10.1007/978-81-322-0786-3
Quiroga G, Erice G, Aroca R, Chaumont F, Ruiz-Lozano JM. Enhanced drought stress tolerance by the arbuscular mycorrhizal symbiosis in a drought-sensitive maize cultivar is related to a broader and differential regulation of host plant aquaporins than in a drought-tolerant cultivar. Frontiers in Plant Science. 2017;19(8):1056. https://doi.org/10.3389/fpls.2017.01056
Khan N, Bano A, Ali S, Babar MA. Crosstalk amongst phytohormones from planta and PGPR under biotic and abiotic stresses. Plant Growth Regulation. 2020;90(2):189-203. https://doi.org/10.1007/s10725-020-00571-x
Chandra D, Srivastava R, Glick BR, Sharma AK. Rhizobacteria producing ACC deaminase mitigate water-stress response in finger millet (Eleusine coracana (L.) Gaertn.). 3 Biotech. 2020;10(2):1-5. https://doi.org/10.1007/s13205-019-2046-4
Abbaspour H, Saeidi-Sar S, Afshari H, Abdel-Wahhab MA. Tolerance of mycorrhiza infected pistachio (Pistacia vera L.) seedling to drought stress under glasshouse conditions. Journal of Plant Physiology. 2012;1;169(7):704-49. https://doi.org/10.1016/j.jplph.2012.01.014
Bárzana G, Aroca R, Bienert GP, Chaumont F, Ruiz-Lozano JM. New insights into the regulation of aquaporins by the arbuscular mycorrhizal symbiosis in maize plants under drought stress and possible implications for plant performance. Molecular Plant-Microbe Interactions. 2014;27(4):349-63. https://doi.org/10.1094/MPMI-09-13-0268-R
Ashraf MH, Harris PJ. Photosynthesis under stressful environments: an overview. Photosynthetica. 2013;1;51(2):163-90. https://doi.org/10.1007/s11099-013-0021-6
Yooyongwech S, Samphumphuang T, Tisarum R, Theerawitaya C, Cha-Um S. Arbuscular mycorrhizal fungi (AMF) improved water deficit tolerance in two different sweet potato genotypes involves osmotic adjustments via soluble sugar and free proline. Scientia Horticulturae. 2016;(26)198:107-17. https://doi.org/10.1016/j.scienta.2015.11.002
Burghelea C, Zaharescu DG, Dontsova K, Maier R, Huxman T, Chorover J. Mineral nutrient mobilization by plants from rock: influence of rock type and arbuscular mycorrhiza. Biogeochemistry. 2015;124(1):187-203. https://doi.org/10.1007/s10533-015-0092-5
Morte A, Navarro-Ródenas A, Nicolás E. Physiological parameters of desert truffle mycorrhizal Helianthemun almeriense plants cultivated in orchards under water deficit conditions. Symbiosis. 2010;52(2):133-39. https://doi.org/10.1007/s13199-010-0080-4
Augé RM. Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza. 2001; 1;11(1):3-42. https://doi.org/10.1007/s005720100097
Amiri R, Nikbakht A, Etemadi N. Alleviation of drought stress on rose geranium [Pelargonium graveolens (L.) Herit. ] in terms of antioxidant activity and secondary metabolites by mycorrhizal inoculation. Scientia Horticulturae. 2015;14(197):373-80. https://doi.org/10.1016/j.scienta.2015.09.062
Wu QS, Zou YN, Huang YM. The arbuscular mycorrhizal fungus, Diversispora spurca ameliorates effects of waterlogging on growth, root system architecture and antioxidant enzyme activities of citrus seedlings. Fungal Ecology. 2013;6(1):37-43. https://doi.org/10.1016/j.funeco.2012.09.002
Sperdouli I, Moustakas M. Interaction of proline, sugars and anthocyanins during photosynthetic acclimation of Arabidopsis thaliana to drought stress. Journal of Plant Physiology. 2012;169(6):577-85. https://doi.org/10.1016/j.jplph.2011.12.015
Sharma S, Villamor JG, Verslues PE. Essential role of tissue-specific proline synthesis and catabolism in growth and redox balance at low water potential. Plant Physiology. 2011;157(1):292-304. https://doi.org/10.1104/pp.111.183210
Aroca R, Porcel R, Ruiz-Lozano JM. Regulation of root water uptake under abiotic stress conditions. Journal of Experimental Botany. 2012;1;63(1):43-57. https://doi.org/10.1093/jxb/err266
Zhang Z, Zhang J, Huang Y. Effects of arbuscular mycorrhizal fungi on the drought tolerance of Cyclobalanopsis glauca seedlings under greenhouse conditions. New Forests. 2014;45(4):545-56. https://doi.org/10.1007/s11056-014-9417-9
Bacon CW, White JF. Functions, mechanisms and regulation of endophytic and epiphytic microbial communities of plants. Symbiosis. 2016;68(1-3):87-98. https://doi.org/10.1007/s13199-015-0350-2
Huang YM, Zou YN, Wu QS. Alleviation of drought stress by mycorrhizas is related to increased root H 2 O 2 efflux in trifoliate orange. Scientific Reports. 2017 Feb 8;7(1):1-9. https://doi.org/10.1038/srep42335
Akram NA, Shafiq F, Ashraf M. Ascorbic acid-a potential oxidant scavenger and its role in plant development and abiotic stress tolerance. Frontiers in Plant Science. 2017 Apr 26;8:613. https://doi.org/10.3389/fpls.2017.00613
Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany. 2012;2012. https://doi.org/10.1155/2012/217037
Li J, Wang Y, Pritchard HW, Wang X. The fluxes of H 2 O 2 and O 2 can be used to evaluate seed germination and vigor of Caragana korshinskii. Planta. 2014;239(6):1363-73. https://doi.org/10.1007/s00425-014-2049-7
Ruiz-Sánchez M, Aroca R, Muñoz Y, Polón R, Ruiz-Lozano JM. The arbuscular mycorrhizal symbiosis enhances the photosynthetic efficiency and the antioxidative response of rice plants subjected to drought stress. Journal of Plant Physiology. 2010;167(11):862-69. https://doi.org/10.1016/j.jplph.2010.01.018
Gharibi S, Tabatabaei BE, Saeidi G, Goli SA. Effect of drought stress on total phenolic, lipid peroxidation and antioxidant activity of Achillea species. Applied Biochemistry and Biotechnology. 2016;178(4):796-809. https://doi.org/10.1007/s12010-015-1909-3
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Copyright (c) 2021 Jaagriti Tyagi, Neeraj Shrivastava, AK Sharma, Ajit Varma, Ramesh Pudake
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