Avirulence gene based RFLP and rep-PCR distinguish the genetic variation of Xanthomonas oryzae pv. oryzae pathotypes in Bangladesh

Authors

  • Mohammad Mahbubul Haque Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh-2202, Bangladesh
  • Md. Mostafa Masud Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0000-0002-1378-6642
  • Samrin Bashar Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Muhammad Iqbal Hossain Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Md. Zahangir Alam Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0000-0002-2574-4394
  • Md. Rashidul Islam Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0000-0002-0790-6521

DOI:

https://doi.org/10.14719/pst.1369

Keywords:

RFLP, Rep-PCR, X. oryzae pv. oryzae, Pathotypes

Abstract

Bacterial blight (BB) caused by X. oryzae pv. oryzae is one of the devastating diseases of rice mostly in Asia. Genomes of X. oryzae pv. oryzae is highly variable due to rearrangement of the large contents of transposable elements and dynamic changes of X. oryzae pv. oryzae population regulated efficiency of the control measures used for BB management of rice worldwide. In this study, genetic variation of X. oryzae pv. oryzae pathotypes of Bangladesh was studied using aviruelnce gene based RFLP and rep-PCR techniques aimed to formulate pathogen targeted effective control measures against BB of rice. Eight pathotypes of X. oryzae pv. oryzae field isolates were identified based on their reactions against 10 Near Isogenic Lines (NILs). Among eight pathotypes, pathotypes IV and V contained higher number of isolates which were 30.13% and 23.01% respectively while pathotype VIII revealed as minimum containing only 2.51% of total isolates. These eight pathotypes were studied for their genetic variation by RFLP using avrBs3 repeat domain as probe. The results conceded that Bangladeshi X. oryzae pv. oryzae strains seem carrying a minimum of two and maximum of nine avrBs3 family genes homologs. The resistance phenotype on IRBB7 and IRBB10 NILs also indicated presence of two major avrBs3 family genes viz. avrxa7 and avrXa10 in some pathotypes. Relationship of phylogenicity exhibited that X. oryzae pv. oryzae pathotypes assorted into two RFLP haplotypes as well as these haplotypes are largely distributed in Bangladesh. Phylogenetic analyses carried out by (REP, ERIC), rep-PCR and BOX depicted the presence of two main molecular haplotypes of X. oryzae pv. oryzae pathotypes. The relationship between pathotypes and molecular haplotypes of X. oryzae pv. oryzae in Bangladesh indicated that the same lineage possesses different pathotypes and different lineage possesses different pathotypes. The results indicated that eight different pathotypes might have originated from common inherited haplotypes with a wide genetic variation.

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Author Biographies

Mohammad Mahbubul Haque, Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh-2202, Bangladesh

Scientific Officer,

Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture (BINA)

Md. Mostafa Masud, Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Masters student,

Department of Plant Pathology, Bangladesh Agricultural University(BAU).

Samrin Bashar, Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Masters student

Department of Plant Pathology, Bangladesh Agricultural Unversity (BAU)

Muhammad Iqbal Hossain, Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

PhD student

Department of Plant Pathology, Bangladesh Agricultural University(BAU)

Md. Zahangir Alam , Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Assistant Professor,

Department of Plant Pathology, Bangladesh Agricultural university (BAU)

Md. Rashidul Islam, Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Professor

Department of Plant Pathology, Bangladesh Agricultural University (BAU)

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Published

01-01-2022

How to Cite

1.
Haque MM, Masud MM, Bashar S, Hossain MI, Alam MZ, Islam MR. Avirulence gene based RFLP and rep-PCR distinguish the genetic variation of Xanthomonas oryzae pv. oryzae pathotypes in Bangladesh. Plant Sci. Today [Internet]. 2022 Jan. 1 [cited 2024 Nov. 4];9(1):29-40. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1369

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