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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Effect of biofertilizers on the survival and growth of air-layered saplings of West Indian cherry (Malpighia glabra L.)

DOI
https://doi.org/10.14719/pst.12595
Submitted
5 November 2025
Published
04-02-2026

Abstract

This study evaluated the efficacy of air layering in combination with biofertilizer treatments for the successful propagation of West Indian cherry (Malpighia glabra L.) during 2022–2023 at the North Farm, School of Agricultural Sciences, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India. Air layering was performed on a 9-year-old tree using 1-year-old, pencil-thick shoots of 60 cm length. Once rooted, the air layers were gradually detached from the mother plant and transplanted into polybags. Biofertilizer treatments were applied to the potting medium and as soil drenches, including Trichoderma viride (3 g L-1), vermiwash (1 %), humic acid (20 g L-1), Azospirillum brasilense (2 g kg-1 planting medium), plant growth-promoting rhizobacteria (PGPR; 10 g plant-1) and vesicular-arbuscular mycorrhiza (VAM; 100 g plant-1), along with an untreated control. Data recorded at 60 days after detachment (DAD) showed that vermiwash @ 1 % (T3) significantly enhanced shoot and root growth parameters. It resulted in the highest plant height (45.23 cm), number of leaves (38.22), number of shoots (6.45), survival percentage (81.04 %), root length (13.80 cm), primary roots (26.31), secondary roots (78.23) and root diameter (4.88 mm). The improved performance under vermiwash treatment is attributed to its rich content of plant growth regulators, enzymes, micronutrients and beneficial microbes, which positively influenced both vegetative and root development. The results underscore the potential of integrating air layering with nutrient-enriched organic treatments like vermiwash to enhance the propagation efficiency and field establishment of West Indian cherry. The objective of this study was to identify an effective biofertilizer-based strategy to enhance survival, growth and nursery establishment of air-layered West Indian cherry saplings.

References

  1. 1. Dey K, Ghosh A, Bauri FK, Bhowmick N, Dey AN, Mishra DK, et al. Barbados cherry: agriculture, breeding, utilization and role in nutritional and economic security – a review. Agric Rev. 2018;39(2):144–50. https://doi.org/10.18805/ag.R-1764
  2. 2. Kirker CL, Newman M. Cherry. London: Reaktion Books; 2021.
  3. 3. Cruz RG, Beney PS, Lira TM, Vieira S. Comparison of the antioxidant property of acerola extracts with synthetic antioxidants using an in vivo method with yeasts. Food Chem. 2019;277:698–705. https://doi.org/10.1016/j.foodchem.2018.10.099
  4. 4. Prakash A, Baskaran R. Acerola, an untapped functional superfruit: a review on latest frontiers. Int J Food Sci Technol. 2018;55:3373–84. https://doi.org/10.1007/s13197-018-3309-5
  5. 5. Hazarika J, Hazarika DN, Langthasa S. Standardization of propagation method of custard apple by air layering. J Agri Search. 2021;8(3):222–28.
  6. 6. Hazarika BN, Ansari S. Biofertilizers in fruit crops – a review. Agric Rev. 2007;28(1):69–74.
  7. 7. Eşitken A, Yıldız HE, Ercisli S, Dönmez MF, Turan M, Güneş A. Effects of plant growth promoting bacteria on yield, growth and nutrient contents of organically grown strawberry. Sci Hortic. 2010;124(1):62–66. https://doi.org/10.1016/j.scienta.2009.12.012
  8. 8. Verma S, Babu A, Patel A, Singh SK, Pradhan SSP, Verma SK, et al. Significance of vermiwash on crop production: a review. J Pharmacogn Phytochem. 2018;7(2):297–301.
  9. 9. Sharma BL, Singh SP, Sharma ML. Bio-degradation of crop residues by Trichoderma species vis-à-vis nutrient quality of the prepared compost. Sugar Tech. 2012;14(2):174–80. https://doi.org/10.1007/s12355-011-0125-x
  10. 10. Vázquez MB, Barrera V, Bianchinotti V. Molecular identification of Trichoderma harzianum isolates and their ability to detoxify metsulfuron methyl. Botany. 2015;93(11):793–800. https://doi.org/10.1139/cjb-2015-0085
  11. 11. Piccolo A. The nature of soil organic matter and innovative soil managements. In: Carbon sequestration in agricultural soils. Berlin: Springer; 2012. p. 1–19. https://doi.org/10.1007/978-3-642-23385-2_1
  12. 12. Kumar R. Off-season air layering in litchi: a prudent and efficient approach. Technical Bulletin No. 6. Muzaffarpur: NRC for Litchi; 2012.
  13. 13. Domenico P. Trichoderma viride inoculation increases vitamin C in Kalanchoe leaves and defense against Pythium sp. GSC Adv Res Rev. 2020;5(2):89–96. https://doi.org/10.30574/gscarr.2020.5.2.0108
  14. 14. Rajasooriya APS, Karunarathna B. Application of vermiwash on growth and yield of green gram. AGRIEAST J Agric Sci. 2020;14(2):31–37. https://doi.org/10.4038/agrieast.v14i2.95
  15. 15. Chakraborty S, Rajkumar M. Effect of growth regulators and organic substances on rooting of grape cuttings. Asian J Sci Technol. 2018;9(7):8418–21.
  16. 16. Singh DI, Sharma TR, Nagar OP, Pal S. Influence of PGRs and growing media on marcottage in pomegranate. Asian J Sci Technol. 2021;9(3):255–60.
  17. 17. Hemant R, Kirti S, Manju N. Effect of organic manure and biofertilizers on growth and yield of sweet orange. Asian J Sci Technol. 2020;9(4):2064–70. https://doi.org/10.20546/ijcmas.2020.904.247
  18. 18. Hakim A, Jaganath S, Honnabyraiah MK, Mohan Kumar S, Anil Kumar S, Dayamani KJ. Influence of biofertilizer and auxin on growth and rooting of pomegranate cuttings. Int J Curr Microbiol Appl Sci. 2018;7(2):1187–93. https://doi.org/10.20546/ijcmas.2018.702.146
  19. 19. Makkar C, Singh J, Parkash C. Vermicompost and vermiwash as supplements to improve plant growth. Int J Recycl Org Waste Agric. 2017;6(3):203–18. https://doi.org/10.1007/s40093-017-0168-4
  20. 20. Bashir MA, Mushtaq A, Muhammad AA. Effect of potting media on growth of rooted jojoba cuttings. Int J Agric Biol. 2007;9(2):147–51.
  21. 21. Hiradeve PN, Deotale RD, Deogirkar MS, Gaikwad SB. Effect of vermicompost wash on groundnut. J Soils Crops. 2011;21(2):266–72.
  22. 22. Jaybhaye MM, Bhalerao SA. Influence of vermiwash on seedling growth of green gram and black gram. Int J Curr Microbiol Appl Sci. 2015;4(9):635–43.
  23. 23. Vijantie R, Awadhpersad R, Ori L, Ansari AA. Effect of vermiwash on tomato productivity. Asian J Agric. 2021;5(1):29–34. https://doi.org/10.13057/asianjagric/g050105
  24. 24. Sundararasu K, Jeyasankar A. Effect of vermiwash on growth and yield of brinjal. Asian J Sci Technol. 2014;5(3):171–73.
  25. 25. Taleshi K, Shokohfar A, Rafiee M, Noormahamadi G, Sakinejhad T. Effect of vermicompost on safflower. Int J Agron Plant Prod. 2011;2(1):15–22.
  26. 26. Murugesan MP. Influence of vermiwash on cowpea and rice. IOSR J Pharm. 2012;2(6):31–34. https://doi.org/10.9790/3013-26403134
  27. 27. Kumar P, Shekhar C, Basoli M, Kumar V. Sequential vermiwash spray in gladiolus. Ann Hortic. 2013;6(1):71–75.
  28. 28. Kapase PV, Deotale RD, Sawant PP, Sahane AN, Banginwar AD. Effect of humic acid and vermiwash on chickpea. J Soils Crops. 2014;24(1):107–14.
  29. 29. Sathe TV, Patil SS. Vermiwash for mango growth and yield. Indian J Appl Res. 2014;4(6):535–36.

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