Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Early Access

Study of in vitro propagation and acclimatisation in Polianthes tuberosa cv. Pink Sapphire

DOI
https://doi.org/10.14719/pst.12743
Submitted
15 November 2025
Published
28-01-2026

Abstract

Polianthes tuberosa cv. Pink Sapphire is one of the beautiful commercial flower varieties newly imported into Vietnam. However, detailed studies on the micropropagation and acclimatisation of this flower species to the local environment are lacking. To meet the production and commercial demand of this flower variety in Vietnams’ An Giang province, the study was conducted to select optimal media for in vitro propagation and suitable environments for seedling acclimatisation under greenhouse conditions. Five experiments were encompassed in this study, consisting of sterilisation of initial explants, in vitro shoot regeneration from bulb scales, in vitro shoot multiplication, complete plantlet generation and acclimatisation of seedlings in the greenhouse. The results demonstrated that the most effective sterilisation method was using 0.2 % HgCl2 for 15 min and then 10 % Ca(OCl)2 for 10 min. Four-scaling bulbs cultured on MS media containing 2.5 mg/L BAP and 0.5 mg/L NAA were recommended for in vitro shoot regeneration. The best medium for in vitro shoot multiplication was MS medium supplemented with 2 mg/L BAP and 0.5 mg/L NAA. The suitable medium for complete plantlet regeneration was MS medium. To acclimatise Pink Sapphire in the greenhouse, the optimal substrate was a mixture of rice husk ash, coconut coir and sand in equal parts (1:1:1). The findings of this study provide a foundation for the rapid development of the P. tuberosa cv. Pink Sapphire variety in An Giang province of Vietnam.

References

  1. 1. Shen TM, Huang KL, Shen RS, Du BS. Breeding of dwarf tuberose (Polianthes tuberosa L.). Acta Hortic. 2003;624:73–6. https://doi.org/10.17660/ActaHortic.2003.624.9
  2. 2. Kumar M, Sirohi U, Malik S, Kumar S, Ahirwar GK, Chaudhary V, et al. Methods and factors influencing in vitro propagation efficiency of ornamental tuberose (Polianthes species): A systematic review of recent developments and future prospects. Horticulturae. 2022;8:998. https://doi.org/10.3390/horticulturae8110998
  3. 3. Mandal R, Singh NP, Mukopadayay S. A review on ethnomedicinal properties on Polianthes tuberosa L. Int J Health Sci. 2022;6:S3:9728–43. https://doi.org/10.53730/ijhs.v6nS3.8550
  4. 4. Cheesman L, Finnie JF, Van-Staden J. Eucomis zambesiaca Baker: Factors affecting in vitro bulblet induction. South Afr J Bot. 2010;76:543–9. https://doi.org/10.1016/j.sajb.2010.04.004
  5. 5. Nalousi AM, Hatamzadeh A, Azadi P, Mohsenpour M, Lahiji HS. A procedure for indirect shoot organogenesis of Polianthes tuberosa L. and analysis of genetic stability using ISSR markers in regenerated plants. Sci Hortic. 2019;244:315–21. https://doi.org/10.1016/j.scienta.2018.09.066
  6. 6. Ahmad MS, Ahmad T, Zaidi N, Nasir IA. High frequency in vitro propagation of Polianthes tuberosa. Pak J Sci Ind Res. 2006;49:344–8.
  7. 7. Duyen NTM, Phong TT, Tam BP. Effects of bulb size and fertilizers on growth, yield and color of Polianthes tuberosa Pink Sapphire. Asian J Plant Sci. 2025;24:60–72. https://doi.org/10.3923/ajps.2025.60.72
  8. 8. Taha H, Ghazy UM, Gabr AMM, EL-Kazzaz AAA, Ahmed EAMM, Haggag KM. Optimization of in vitro culture conditions affecting propagation of mulberry plant. Bull Natl Res Cent. 2020;44:60. https://doi.org/10.1186/s42269-020-00314-y
  9. 9. Bello OA, Esan EB, Obembe OO. Establishing surface sterilization protocol for nodal culture of Solanecio biafrae. IOP Conf Ser: Earth Environ Sci. 2018;210:012007. https://doi.org/10.1088/1755-1315/210/1/012007
  10. 10. Gajbhiye SS, Tripathi MK, Vidya-Shankar M, Singh M, Baghel BS, Tiwari S. Direct shoot organogenesis from cultured stem disc explants of tuberose (Polianthes tuberosa Linn.). J Agric Technol. 2011;7:695–709.
  11. 11. Aslam F, Naz S, Tariq A, Ilyas S, Shahzadi K. Rapid multiplication of ornamental bulbous plants of Lilium orientalis and Lilium longiflorum. Pak J Bot. 2013;45:2051–5.
  12. 12. Patil M, Bharathi TU, Usharani TR, Kumar R, Kulkarni BS. Standardization of sterilization protocol for explants and its suitability for direct organogenesis in tuberose cv. Arka Vaibhav. J Hortic Sci. 2023;18:173–80. https://doi.org/10.24154/jhs.v18i1.2160
  13. 13. Nugrahani P, Purnobasuki H, Ansori ANM, Anuchai J, Priyanto AD. Effect of different strengths of MS media and BAP on banana plantlet growth in vitro. Sarhad J Agric. 2024;40:1110–7. https://doi.org/10.17582/journal.sja/2024/40.4.1110.1117
  14. 14. Sugiyono, Dewi PS, Prasetyo R. Banana cultivars microshoot introduction and plantlet formation using cytokinin and auxin. Caraka Tani J Sustain Agric. 2021;36:249–58. https://doi.org/10.20961/carakatani.v36i2.50425
  15. 15. Justine AK, Kaur N, Savita, Pati PK. Biotechnological interventions in banana: Current knowledge and prospects. Heliyon. 2022;8:e11636. https://doi.org/10.1016/j.heliyon.2022.e11636
  16. 16. Sanavy SAMM, Moeini MJ. Effects of different hormone combinations and planting beds on growth of single nodes and plantlets resulted from potato meristem culture. Plant Tissue Cult Biotechnol. 2003;13:145–50.
  17. 17. Wiendi NMA, Putri DD. In vitro adventitious shoot proliferation of three basil species (Ocimum sp. L.) by addition of naphthalene acetic acid (NAA) and benzyl amino purine (BAP). J Trop Crop Sci. 2017;4:108–15. https://doi.org/10.29244/jtcs.4.3.108-115
  18. 18. Naz S, Aslam F, Ilyas S, Shahzadi K, Tariq A. In vitro propagation of tuberose (Polianthes tuberosa). J Med Plants Res. 2012;6:4107-12. https://doi.org/10.5897/JMPR12.647
  19. 19. Ashrafi K, Iqrar S, Saifi M, Khan S, Qamar F, Quadri SN, et al. Influence of plant growth regulators on glandular trichome density and steviol glycosides accumulation in Stevia rebaudiana. ACS Omega. 2022;7:30967–77. https://doi.org/10.1021/acsomega.2c02957
  20. 20. Rezali NI, Sidik NJ, Saleh A, Osman NI, Adam NAM. The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme. Asian Pac J Trop Biomed. 2017;7:151–6. https://doi.org/10.1016/j.apjtb.2016.11.019
  21. 21. Dönmez D, Erol HM, Biçen B, Şimşek Ö, Kaçar-Aka Y. The effects of different strength of MS media on in vitro propagation and rooting of Spathiphyllum. Anadolu J Agric Sci. 2022;37:583–92. https://doi.org/10.7161/omuanajas.1082219
  22. 22. Rodboot N, Te-chato S, Yenchon S. Influence of MS medium strengths and types on in vitro shoot multiplication and development of Nymphaea colorata. ASEAN J Sci Tech Report. 2025;28:e258176. https://doi.org/10.55164/ajstr.v28i4.258176
  23. 23. Asmono SL, Rahmawati, Sjamsijah N. The effect of Murashige and Skoog (MS) modified medium and several types of auxins on the growth of Stevia rebaudiana Bertoni in vitro. IOP Conf Ser: Earth Environ Sci. 2021;672:012001. https://doi.org/10.1088/1755-1315/672/1/012001
  24. 24. Khiem DV, Huyen PX, Hang NTT, Hoang NTP. In vitro regeneration and acclimatization of Anthurium scherzerianum Schott plants. AJB. 2022;44:99–109. https://doi.org/10.15625/2615-9023/16548
  25. 25. Nadal MC, de Assis AM, Schuch MW, de Faria RT. Grape-based residue as a substrate in Oncidium baueri Lindl. acclimation. Ornam Hortic. 2022;28:239–45. https://doi.org/10.1590/2447-536x.v28i2.2477

Downloads

Download data is not yet available.