Influence of nutrient sources and environmental conditions on vegetative growth of fungus, Erythricium salmonicolor (Berk. & Broome) Burdsall in cocoa
DOI:
https://doi.org/10.14719/pst.1295Keywords:
Corticiaceae, Fungal nutrition, Growth condition, Pink diseaseAbstract
Erythricium salmonicolor is the cause of pink disease in cocoa in the humid tropics. The growth of 4 isolates (ES-WR006, ES-WR008, ES-ER009 and ES-ER010) of the fungus on different sources of nutrient (carbohydrate, amino acid and vitamin) and environmental conditions (temperature, pH and light) were evaluated in the laboratory. The pink disease isolates utilised the nutrients to different extent, with the best radial mycelial growth rate achieved with isolate ES-WR008 followed by ES-WR006. None of the isolates produced spores in the laboratory. The isolates utilised polysaccharides as best as disaccharides and monosaccharaides. Among 7 amino acids assayed, aspartic acid was the most promising for vegetative growth. The best radial mycelial growth on vitamins was attained on folic acid and riboflavin. The minimum, optimum and maximum temperatures for growing the fungus were 18 °C, 28 °C and 34 °C respectively. Survival of E. salmonicolor isolates within the temperature range of 18-34 °C is consistent with temperatures for growing cocoa in Ghana. The isolates also grew well within a pH range of 4-8, with the best growth at pH 6. Light quality and duration influenced the vegetative growth of the isolates. Pink disease isolates maintained in 24 hr darkness or 24 hr light performed better than in alternating 12 hr darkness and 12 hr light. There was a significant (p<0.05) repressive effect of the alternating dark and light exposures on vegetative growth of the fungus. The implications of the results for efficient management of the cocoa disease are discussed.
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References
Old KM, Davison EM. Canker diseases of Eucalyptus. In: Keane PJ, Kile GA, Podger FD, Brown BN, Editors. Diseases and Pathogens of Eucalypts. CSIRO Publishing, Collingwood, Australia. 2000; p. 245-46.
Kwarteng FG, Cornelius E, Acquah KK, Asare-Kumi E. Morphological and Molecular Identification of the fungus associated with Pink Disease of cocoa (Theobroma cacao L.) in the Eastern Region of Ghana. International Journal of Pathogen Research. 2018;1(1):1-8. https://doi.org/10.9734/ijpr/2018/v1i11161
Akrofi AY, Amoako-Attah I, Assuah MK, Kumi-Asare E. Pink Disease caused by Erythricium salmonicolor (Berk. & Broome) Burdsall: an epidemiological assessment of its potential effect on cocoa production in Ghana. Journal of Plant Pathology and Microbiology. 2014; 5:215. https://doi.org/10.4172/2157-7471.1000215.
Roux J, Coetzee MPA. First report of pink disease on native trees in South Africa and phylogenetic placement of Erythricium salmonicolor in the Homobasidiomycetes. Plant Disease. 2005;89:1158-63. https://doi.org/10.1094/PD-89-1158.
Moraes SRG, Furtado GQ, Scaloppi ÉAG, Barreto M, Júnior M, Sidnei N. Sporulation of Erythricium salmonicolor and its anamorphic stage Necator decretus, causal agent of citrus pink disease in Brazil. Fitopatologia Brasileira. 2006; 31(5):519. https://doi.org/10.1590/S0100-41582006000500016.
Opoku IY, Akrofi AY, Osei-Bonsu K, Acheampong K. An outbreak of pink disease in Ghana. In: Proc. of the 13th Int. Cocoa Res. Conf, Kota Kinabalu, Sabah, Malaysia. 2001; p. 761-67.
Akrofi AY, Amoako-Attah I, Acheampong K, Assuah MK, Melnick RL. Fruit and canopy pathogens of unknown potential risk. In: (Bailey B, Meinhardt L editors. Cacao diseases: a history of old enemies and new encounters. Springer International Publishing, Switzerland. 2016; p. 361-82. https://doi.org/10.1007/978-3-319-24789-2_11.
Schneider-Christians J, Fliege F, Schlosser E. On the release, survival and importance of basidiospores of Corticium salmonicolor, the pathogen causing pink disease. J Plant Dis Prot. 1986;93:397-403.
Castro-Moretti FR, Gentzel IN, Mackey D, Alonso AP. Metabolomics as an emerging tool for the study of plant-pathogen interactions. Metabolites. 2020;10(2):52. doi: 10.3390/metabo10020052. PMID: 32013104; PMCID: PMC7074241. https://doi.org/10.3390/metabo10020052
Gao L. Optimization of culture medium for sporulation and biomass production of a nematophagous fungus: consideration of nutritional and environmental conditions, Journal of Phytopathology. 2015;163:536-42. https://doi.org/10.1111/jph.12351
Kavanagh K. Fungi: Biology and Applications, 3rd Edition. John Wiley & Sons, Inc. 2018. 35p.
Formela-Luboinska M., Remlein-Starosta D, Wa´skiewicz A, Karolewski Z, Bocianowski J, Stepie´n ? et al. The role of saccharides in the mechanisms of pathogenicity of Fusarium oxysporum f. sp. lupini in Yellow Lupine (Lupinus luteus L.). Int J Mol Sci. 2020; 21:7258. https://doi.org/10.3390/ijms21197258
Li Y, Yang C, Ahmad H, Maher M, Fang C, Luo J. Benefiting others and self: Production of vitamins in plants. J Integr Plant Biol. 2021;63:210-27. https://doi.org/10.1111/jipb.13047
Sun Y, Wang M, Mur LAJ, Shen Q, Guo S. Unravelling the roles of nitrogen nutrition in plant disease defences. Int J Mol Sci. 2020;21(2):572. doi: 10.3390/ijms21020572. PMID: 31963138; PMCID: PMC7014335. https://doi.org/10.3390/ijms21020572
Bani M; Pérez-De-Luque A; Rubiales D; Rispail N. Physical and chemical barriers in root tissues contribute to quantitative resistance to Fusarium oxysporum f. sp. pisi in pea. Front. Plant Sci. 2018; 9:199. https://doi.org/10.3389/fpls.2018.00199
García-Guzmán G. Environmental factors associated with disease incidence in plant species from a Mexican seasonal tropical dry forest. J Torrey Bot Soc. 2016; 143(3):254-64. https://doi.org/10.3159/TORREY-D-15-00055.1
Pokhrel B. Effects of environmental factors on crop diseases. J Plant Pathol Microbiol. 2021; 12:553.
Adebayo-Tayo BC, Ugwu EE, Musa H. Physiological requirement for growth and extracellular polysaccharides (EPS) production by Marasmius sp. and Fomes sp. (A comparative study). J Microbiol Biotech Res. 2013;3(5):1-11.
Gomori G. Preparation of buffers for use in enzyme studies. 2008. Available from: http://2008.igem.org/wiki/images/8/84/Protein_Buffers.pdf (cited: 2018 July 3)
Manjunathan J. and Kaviyarasan V. Studies on the growth requirements of Lentinus tuberregium (Fr.), an edible mushroom. Middle-East Journal of Scientific Research. 2010;5(2):81-85.
Gbolagade JS, Fasidi IO, Ajayi EJ, Sobowale AA. Effect of physico-chemical factors and semi-synthetic media on vegetative growth of Lentinus subnudus (Berk.), an edible mushroom from Nigeria. Food Chemistry. 2016; 99:742-47. (Britain) https://doi.org/10.1016/j.foodchem.2005.08.052
Sánchez ÓJ and. Montoya S. Assessment of polysaccharide and biomass production from three white-rot fungi by solid-state fermentation using wood and agro-industrial residues: A Kinetic Approach. Forests. 2020;11:1055. https://doi.org/10.3390/f11101055
Aneja KR, Mehrotra RS. Fungal Diversity and Biotechnology. New Age international Publishers, India. 2011; p. 93-100.
Lu WY, GQ Lin, Hi Yu, AM Tong, JH Xu. Practical Methods for Biocatalysis and Biotransformations. Whittall J, Sutton PW. Editors. John Wiley & Sons. 2009; p. 236-39. ISBN 978-0-470-74859-6.
Lilly VG, Barnett HL. The utilisation of sugars by fungi. West Virginia. University Agricultural Experimental Station Bulletin. 1953;262:1-58. https://doi.org/10.33915/agnic.362T
Adebayo-Tayo BC, Ugwu EE. Influence of different nutrient sources on exopolysaccharide production and biomass yield by submerged culture of Trametes versicolor and Coprinus sp. Assumption University Journal of Technology. 2011;15(2):63-69.
Lazarevi? J, Stoji?i? D, Ke?a N. Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrhizal isolates from Pinus heldreichii forest. Forest Systems. 2016;25(1):48-58. https://doi.org/10.5424/fs/2016251-07036.
Kemmitt SJ, Wright D, Jones DL. Soil acidification used as a management strategy to reduce nitrate losses from Agricultural lands. Soil biology and Biochemistry. 2005;37:867-75 https://doi.org/10.1016/j.soilbio.2004.10.001
Luz EDMN. Comparacao Morfologica e Patologica de Isolados de Corticium salmonicolor Berk and Br. de Cacaueiro na Bahia, Brasil (morphological and pathological comparison of corticium salmonicolor Berk and Br, isolates from cacao in Bahia, Brazil). Revista-Theobroma. 1983;13(3):165.
Verma KS, Munjal RL. Studies on the toxicity of various fungicidal sprays and paints on the control of pink disease (Corticium salmonicolor Ber. and Br.) of apple in Rajgarh area of Himachal Pradesh. Pesticides. 1980;14:25.
Shamsuri MH, Omar M, Napi D. Studies on epidemiology of pink disease and the effect of temperature on mycelial growth of Corticium salmonicolor of Hevea Rubber. Journal of Natural Rubber Research. 1997;12(1):58-66.
Anim-Kwapong GJ, Frimpong EB. Vulnerability of agriculture to climate change: Impact of climate change on cocoa production. Vulnerability and Adaptation Assessment under the Netherlands Climate Change Studies Assistance Programme Phase 2 (NCCSAP). New-Tafo Akim: Cocoa Research Institute of Ghana. 2004.
Sanchez F, Honrubia M, Torres P. Effects of pH, water stress and temperature on in vitro growth of ectomycorrhizal fungi from Mediterranean forests. Cryptog Mycol. 2001;22(4):243-58. https://doi.org/10.1016/S0181-1584(01)01076-4
Richie BJ. Mycological media and methods In: Waller, IM, Lenne JM, Waller SJ, Editors. Plant Pathologist Pocketbook, 3rd Edition, CABI Publishing, Wallingford. 2002; p. 516.
Bermudes D, Gerlach VL, Nealson KH. Effects of culture conditions on mycelial growth and luminescence in Panellus stypticus. Mycologia. 1990;82:295-305. https://doi.org/10.1080/00275514.1990.12025884
Corrochano LM. Fungal photobiology: A synopsis. IMA Fungus. 2011;2:25-28. https://doi.org/10.5598/imafungus.2011.02.01.04
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