The mutagenic effect of hydroxylamine hydrochloride on the agronomic traits of Sesame (<i>Sesamum indicum</i> L.)

Authors

  • Abraham Birara Biology Department, College of Natural and Computational Sciences (CNCS), Mekelle University, Mekelle, P.O. Box 231, Ethiopia
  • Muthuswamy Manikandan Biology Department, College of Natural and Computational Sciences (CNCS), Haramaya University, Dire Dawa, P.O. Box 138, Ethiopia
  • Mebeaselassie Andargie Biology Department, College of Natural and Computational Sciences (CNCS), Haramaya University, Dire Dawa, P.O. Box 217, Ethiopia

DOI:

https://doi.org/10.14719/pst.2014.1.1.13

Keywords:

Hydroxylamine hydrochloride, Mutation, Sesame

Abstract

The effects of mutation induction through the use of a chemical mutagen as a method of improving few agronomic traits in sesame (Sesamum indicum L.) were investigated. Healthy and dry seeds of sesame varieties (Abasena and Kelafo 74) were treated with hydroxylamine hydrochloride (HA) at six different concentrations (0.01, 0.02, 0.03, 0.04, 0.05 % (w/v) and control) with the aim of improving the growth and yield parameters of the plant. Bioassay studies showed highly significant difference in germination percentage of the two varieties under the treatment of the mutagen compared to the control. The results obtained from the quantitative parameters also revealed highly significant increase (P=0.01) in the plant heights, number of seeds/pod, number of capsules/plant, internode length and capsule length with decrease in the concentration of the mutagen. In addition, days to maturity have shown a negative mean shift in all the treatments and days to flowering showed a significant positive mean shift only at 0.02% concentration of HA. The chemical mutagen was therefore found to improve the quantitative traits associated with growth and yield of sesame. The induced variation can be exploited in the evolution of new varieties of sesame with improved agronomic traits.

Downloads

Download data is not yet available.

References

Adamu, A.K., & Aliyu, H. (2007). Morphological effects of sodium azide on tomato (Lycopersicon esculentum Mill). Science World Journal, 2(4), 9-12.

Adegoke, J.A. (1984). Bridge induction by sodium azide in Allium cepa. Nigeria Journal of Genetics, 5, 86-89.

Ajibolu, T.J. (2005). Effects of Chemical Mutagens on Benni Seed (Sesamum indicum L.) B.Sc. (Hons.) Project). Ambrose Alli University, Ekpoma, Nigeria, p. 49.

Akhaury, S.B., Sinha, A.N., & Sinha, A.K. (1996). The effectiveness and efficiency of chemical mutagens on biological parameters in hull-less variety of Hordeum vulgare (L.) Karan 16. Neo Botanica, 4, 1-5.

Aliero, A.A. (2006). Effect of hydroxylamine on the germination and growth of sesame (Sesamum indicum L.). Journal of Plant Sciences, 1(4), 356-361. http://dx.doi.org/10.3923/jps.2006.356.361

Al-Qurainy, F. (2009). Effects of sodium azide on growth and yield traits of Eruca sativa (L.). World Applied Science Journal, 7, 220-226.

Ananthaswamy, H.M., Vakil, V.K., & Shrinivas, A. (1971). Biological and physiological changes in gamma irradiated wheat during germination. Radiation Botany, 11, 1-12. http://dx.doi.org/10.1016/S0033-7560(71)91257-9

Anilakumar, K.R., Pal, A., Khanum, F., & Bawa, A.S. (2010). Nutritional, medicinal and industrial uses of sesame (Sesamum indicum L.) seeds - an overview. Agriculturae Conspectus Scientificus, 75, 159-168.

Ashri, A. (1998). Sesame breeding. In J. Janick (Ed.), Plant Breeding Reviews (pp.79-228). Oxford: Oxford UK.

Bedigian, D. (2003). Evolution of sesame revisited: domestication, diversity and prospects. Genetic Resources and Crop Evolution, 50, 779-787.

Bedigian, D., Siegler, D.S., & Harlan, J.R. (1985). Sesamine, sesamolin and the origin of sesame. Biochemical Systematics and Ecology, 13, 133-139. http://dx.doi.org/10.1016/0305-1978(85)90071-7

Begum, T., & Dasgupta, T. (2010). A comparison on the effect of physical and chemical mutagenic treatments in sesame (Sesamum indicum). Genetics and Molecular Biology, 33(4), 761-766. http://dx.doi.org/10.1590/S1415-47572010005000090. PMid:21637588 PMCid:PMC3036137

Brar, G.S., & Ahuja, K.L. (1979). Sesame. In C.P. Malik (Ed.), Its culture, genetics, breeding and biochemistry (pp. 245–313). Annual Review of Plant Science, Kalyani publishers, New Delhi, India.

Burkill, H.M. (1997). Pedaliaceae In The Useful Plants of West Tropical Africa (pp. 414-423). 2nd ed, Vol. 4, Royal Botanic Garden, Kew.

Chrispeels, M.J., & Varner, J.E. (1967). Gibberellic acid induced synthe-sis and release of L- amylase and ribonuclease by isolated barley aleurone layers. Plant Physiology, 42, 396-406.

Dhanavel, D., Pavadai, P., Mullainathan, L., Mohana, D., Raju, G., Girija, M., & Thilagavathi, C. (2008). Effectiveness and efficiency of chemical mutagens in cowpea (Vigna unguiculata(L.) Walp). African Journal of Biotechnology, 7, 4116-4117. http://dx.doi.org/10.1104/pp.42.3.398

Ganapathy, S., Nirmalakumari, A., Senthil, N., Souframanien, J., & Raveendran, T.S. (2008). Isolation of macromutations and mutagenic effectiveness and efficiency in little millet varieties. World Journal of Agricultural Science, 4, 483-486.

Gupta, P.K., & Yashvir, A.N. (1975). Mutagenic effects of individual and combined treatments gamma rays and EMS in Okra (Abelmoscus esculentus (L.) Moench.). Journal of Cytology and Genetics, 9 & 10, 93-97.

Hoballah, A.A. (1999). Selection and Agronomic evaluation of induced mutant lines of sesame. In Induced Mutations for Sesame Improvement (pp.71-84). IAEA-TECDOC, IAEA, Vienna.

Ilbas, A.I., Eroglu, Y., & Eroglu, H.E. (2005). Effects of the application of different concentrations of nan3 for different times on the morphological and cytogenetic characteristics of barley (Hordeum vulgare L.) seedlings. Journal of Integrative Plant Biology, 47, 1101-1106. http://dx.doi.org/10.1111/j.1744-7909.2005.00137.x

Khan, S., & Goyal, S. (2009). Improvement of mungbean varieties through induced mutations. African Journal of Plant Sciences, 3, 174-180.

Khan, S., Wani, M.R., & Parveen, K. (2004). Induced genetic variability for quantitative traits in Vigna radiata (L.) Wilczek. Pakistan Journal of Botany, 36, 845-850.

Konzak, C.F., Nilan R.A., Wagner J., & Foster R.J. (1965). Efficient chemical mutagenesis. Radiation Botany (suppl.) 5, 49-70.

Krishna, G., Shivashankar, G., & Nath, J. (1984). Mutagenic response of rhodes grass (Chloris gayana Kunth.) to gamma rays. Environmental and Experimental Botany, 24(2), 197-205. http://dx.doi.org/10.1016/0098-8472(84)90022-4

Kulkarni, G.B. (2011). Effect of mutagen on Pollen fertility and seed germination in Horse gram [Macrotyloma uniflorum (Verdcourt)]. Bioscience Discovery, 2(1), 146-150.

Kurobane, I., Yamaguchi, H., Sander, C., & Nilan, R. (1979). The effects of gamma irradiation on the production and secretion of enzymes and enzymatic activities in barley. Environmental and Experimental Botany, 19, 75-84. http://dx.doi.org/10.1016/0098-8472(79)90011-X

Lal, G.M., Toms, B., and Lal, S.S. (2009). Mutagenic Sensitivity in Early Generation in Blackgram. Asian Journal of Agricultural Science, 1, 9-11.

Mahandjiev, A., Kosturkova, G., & Mihov, M. (2001). Enrichment of Pisum sativum gene resources through combined use of physical and chemical mutagens. Israel Journal of Plant Sciences, 49(4), 279-284.

Mahla, S.V.S., Mor, B.R., & Yadava, J.S. (1990). Effect of mutagenes on yield and its component characters in mustard. Haryana Agric University Journal of Research, 8(2), 173-173.

Mann, A., Gbate, M., & Umar, A.N. (2003). Medicinal and economic Plants of Nupe land. Jube- evans books and publications, Bida, Niger state.

Mensah, J.K., & Akomeah, P.A. (1992). Mutagenic effects of hydroxylamine and streptomycin on the growth and seed yield of cowpea (Vigna unguiculata (L.) Walp). Legume Research, 15(1), 39-44.

Mensah, J.K., Eruotor, P.G., Iyeke, J.E., & Ekpekurede, E.O. (2003). Mutagenic effect of hydroxylamine, streptomycin and urea on the agronomic characters of rice (Oryza sativa L. CV ITA 150). Indian Journal of Agricultural Research Karnal-India, 37(2), 88 – 93.

Mensah, J.K., Okooboh, G.O., & Osagie, I.P. (2013). Mutagenic effects of hydroxylamine and hydroquinone on some agronomic and yield characters of soyabean (Glycine max (L.) Merr.). International Journal of Modern Botany, 3(2), 20-24.

Mostafa, G.G. (2011). Effect of sodium azide on the growth and variability induction in Helianthus annuus L. International Journal of Plant Breeding and Genetics, 5, 76-85. http://dx.doi.org/10.3923/ijpbg.2011.76.85

Nerker, Y.S. (1976). Mutation studies in Lathyrus sativus. Indian Journal of Genetics, 36, 323-329.

Oplinger, E.S., Putnam, D.H., Kaminski, A.R., Hanson, C.V., Oelke, E.A., Schulte, E.E., & Doll, J.D. (1990). Sesame. University of Minnesota, USA.

Rachovska, G., & Dimova, D. (2000). Effect of sodium azide and gamma rays on M1 quantitative characteristics of the productivity and their connection with M2 mutation changes in winter common wheat. Rasteniev dni- Nauki, 37, 413-419.

Roychowdhury, B., Dasgupta, T., Das, A., & Das, P.K. (2004). Gamma ray and ethyl methane sulphonate induced chlorophyll mutations in mungbean [Vigna radiata (L.) Wilczek]. Bangladesh Journal of Genetics and Biotechnology, 5, 23-26.

Roychowdhury, R., Datta, S., Gupta, P., & Tah, J. (2012). Analysis of genetic parameters on mutant populations of Mungbean (Vigna radiata L.) after ethyl methane sulphonate treatment. Notulae Scientia Biologicae, 4(1), 137-143.

Sato, M., & Gaul, H. (1967). Effect of ethyle methane sulphonate on the fertility of barley. Radiation Botany, 7, 7-15. http://dx.doi.org/10.1016/0033-7560(67)90028-2

Shah, S.A., Ali, I., & Rahman, K. (1990). Induction and selection of superior genetic variables of oilseed Rape (Brassica napus L.). The Nucleus, 27(1-4), 37-40.

Sharma, S.K. (1990). Mutagenic effectiveness and efficiency of EMS, DES and Gamma rays in lentil. Cytologia, 55, 243-247. http://dx.doi.org/10.1508/cytologia.55.243

Sharma, S.K., Ritu, S., & Pandey, D.P. (2005). Studies on mutagen sensitivity, effectiveness and efficiency in urdbean [Vigna mungo (L.) Hepper]. Indian Journal of Genetics, 65, 20-22.

Siddiq, E.A., & Swaminathan, M.S. (1968). Enhanced mutation induction and recovery caused by NG in Oryza sativa L. Indian Journal of Genetics, 28, 297-300.

Solanki, I.S., & Sharma, B. (1999). Induction and isolation of morphological mutations in different mutagenic damage groups in lentil. Indian Journal of Genetics, 59, 479-485.

Sureja, A.K., & Sharma, R.R. (2000). Genetic variability and heritability studies in garden pea (Pisum sativum L.). Indian Journal of Horticulture, 57, 243 - 47.

Thilagavathi, C., & Mullainathan, L. (2009). Isolation of macro mutants and mutagenic effectiveness, efficiency in black gram [Vigna mungo (L) Hepper]. Global Journal of Molecular Sciences, 4, 76-79.

Uno, G., Storey, R., & Moore, R. (2001). Principles of Botany. Mc Graw Hill New York.

Downloads

Published

24-02-2014

How to Cite

1.
Birara A, Manikandan M, Andargie M. The mutagenic effect of hydroxylamine hydrochloride on the agronomic traits of Sesame (<i>Sesamum indicum</i> L.). Plant Sci. Today [Internet]. 2014 Feb. 24 [cited 2024 Nov. 21];1(1):33-8. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/13

Issue

Section

Research communications

Similar Articles

You may also start an advanced similarity search for this article.