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Plant Science Today (PST)

Research Articles

Vol. 13 No. 3 (2026)

Characterisation of advanced breeding lines for bacterial leaf blight resistance by morpho-molecular screening in rice (Oryza sativa L.)

DOI
https://doi.org/10.14719/pst.13103
Submitted
7 December 2025
Published
18-08-2026 — Updated on 31-08-2026
Versions

Abstract

Sixty advanced breeding lines of rice were evaluated for resistance to bacterial leaf blight (BLB) both phenotypically and genotypically at Regional Agricultural Research Station (RARS), Maruteru, Andhra Pradesh, India during kharif, 2023. Phenotypic screening was performed following the International Rice Research Institute (IRRI), standard evaluation system (SES) scale. Among the sixty entries, seven genotypes, 251-3-3-2, MS-1, NLR-163, NLR-164, NLR-945, CM-469 and BM-587, exhibited a highly resistant (HR) reaction with a score of 1, comparable to the resistant check RP Bio-226. Twenty-two lines expressed BLB severity of 13–25 % (score 5) and were categorised as moderately resistant (MR). Molecular profiling using gene-specific simple sequence repeats (SSR) markers revealed significant variation in resistance gene
distribution. The presence of xa5, xa13, Xa21, Xa4 and Xa10 was detected in 9, 4, 23, 44 and 20 lines respectively. Two-gene combinations were identified in 20 genotypes, whereas 11 genotypes possessed three-gene combinations, highlighting the effectiveness of gene stacking for broad-spectrum BLB resistance. Notably, lines 251-3-3-2, MS-1, NLR-164, NLR-945 and BM-587 exhibited congruence between phenotypic resistance (score 1) and the presence of multiple resistance genes, indicating their potential as elite donors for BLB resistance introgression. Yield performance coupled with gene pyramiding further strengthened the value of several genotypes. The genotypes VP-R-157 (Xa4+xa13) recorded 22.3 g/plant, BM-588 (xa5+Xa21) yielded 21.2 g/plant, while CM-467 (Xa4+xa5+Xa10+Xa21) produced 25.1 g/plant. The genotypes CM-470, CM-446 and CM-449 yielded 26.3 g, 21.0 g and 21.5 g/plant respectively, each carrying the Xa4+xa5 gene combination. These genotypes hold substantial promise for direct varietal release or utilisation in hybridisation programs aimed at developing high-yielding, BLB-resistant rice cultivars.

References

  1. 1. Tannidi B, VGIL, MSA, Laha GS, Sundaram RM, Senguttuvel P, et al. Identification of novel sources of bacterial leaf blight resistance in wild species of rice. Plant Genet Resour. 2025;23(2):129–37. https://doi.org/10.1017/S1479262124000601
  2. 2. Samal P, Babu SC, Mondal B, Mishra SN. The global rice agriculture towards 2050: An inter-continental perspective. Outlook on Agriculture. 2022;51(2):164–72. https://doi.org/10.1177/00307270221088338
  3. 3. Patil B, Jagadeesh GB, Karegowda C, Naik S, Revathi RM. Management of bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae under field condition. J Pharmacogn Phytochem. 2017;6(6):244–6.
  4. 4. Lu Y, Zhong Q, Xiao S, Wang B, Ke X, Zhang Y, et al. A new NLR disease resistance gene Xa47 confers durable and broad-spectrum resistance to bacterial blight in rice. Front Plant Sci. 2022;13:1037901. https://doi.org/10.3389/fpls.2022.1037901
  5. 5. Kauffman HE, Reddy APK, Hsieh S, Merca SD. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Reptr. 1973;57:537–41.
  6. 6. Dellaporta SL, Wood J, Hicks JB. A plant DNA minipreparation: Version II. Plant Mol Biol Rep. 1983;1(4):19–21. https://doi.org/10.1007/BF02712670
  7. 7. Acharya A, Adhikari NR, Amgain RB, Poudel A, Yadav R, Poudyal K. Identification of rice genotypes resistant to bacterial leaf blight disease using SSR markers. J Inst Agric Anim Sci. 2018;35(1):113–20. https://doi.org/10.3126/jiaas.v35i1.22521
  8. 8. Patel AA, Jadeja GC, Subhash N, Shukla YM. Molecular characterization of bacterial leaf blight resistance near isogenic line of rice (Oryza sativa L.) using RAPD and SSR markers. Int J Agric Environ Biotechnol. 2014;7:427–38.
  9. 9. Kushwaha UK, Deo I. Identification of bacterial leaf blight (Xanthomonas oryzae pv. oryzae) resistant genes in rice plants and its validation through molecular markers. J Biotechnol Crop Sci. 2023;11(1–2):16–28.
  10. https://doi.org/10.5958/2582-5089.2023.00003.8
  11. 10. Chukwu SC, Rafii MY, Ramlee SI, Ismail SI, Oladosu Y, Kolapo K, et al. Marker-assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in Putra-1 rice variety. Agronomy. 2020;10(1):42. https://doi.org/10.3390/agronomy10010042
  12. 11. Ashiba R, Aiyanathan KE, Kannan R, Pillai MA. Genotypic assessment of bacterial leaf blight resistance in indigenous rice (Oryza sativa L.) germplasm. Int J Curr Microbiol Appl Sci. 2020;9(7):210–27. https://doi.org/10.20546/ijcmas.2020.907.024
  13. 12. Sahoo SK, Deo I, Prashad H, Kumar A, Kumawat S. Evaluation of rice (Oryza sativa L.) pre-breeding genotypes for resistance to bacterial leaf blight (Xanthomonas oryzae pv. oryzae) disease under field condition. Pharma Innovation. 2023;12(5):1093–5.
  14. 13. Srujana V, Balram M, Srinivas B, Balram N. Phenotypic evaluation of advanced breeding lines for resistance against bacterial leaf blight disease in rice (Oryza sativa L.). Int J Environ Clim Change. 2021;11(12):305–9. https://doi.org/10.9734/ijecc/2021/v11i1230581
  15. 14. Alekya K, Prasanna BL, Balram M, Balram N, Nayak PG, Srinivas B, et al. Phenotypic screening of F4 breeding lines against bacterial blight disease in rice. Int J Environ Clim Change. 2021;11(12):299–304. https://doi.org/10.9734/ijecc/2021/v11i1230580
  16. 15. Sinha S, Sinha S, Kumar M, Singh SP, Kumar A, Kumar A. Screening of restorable WA-CMS rice genotypes for bacterial blight (BB) resistance. Curr J Appl Sci Technol. 2020;39(12):45–52. https://doi.org/10.9734/cjast/2020/v39i1230662
  17. 16. Thanh T, Ton PH, Hai VD, Luong NT, Hai TV, Hein PB, et al. Detection of bacterial leaf blight resistance genes in indigenous glutinous rice landraces. J Hortic Plant Res. 2018;2:1–9.
  18. 17. Khan A, Khare U, Singh S. Molecular marker assisted selection of introgressed four bacterial blight resistance genes (Xa4, Xa7, Xa13 and Xa21) in the genetic background of Mahamaya. Int J Adv Biochem Res. 2023;7(2S):424–27. https://doi.org/10.33545/26174693.2023.v7.i2Sf.246
  19. 18. Song WY, Wang GL, Chen LL, Kim HS, PI LY, Holsten T, et al. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science. 1995;270(5243):1804–6. https://doi.org/10.1126/science.270.5243.1804
  20. 19. Singh S, Sidhu JS, Huang N, Vikal Y, Li Z, Brar DS, et al. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor Appl Genet. 2001;102(6):1011–5. https://doi.org/10.1007/s001220000495
  21. 20. Gu K, Yang B, Tian D, Wu L, Wang D, Sreekala C, et al. R gene expression induced by a type-III effector triggers disease resistance in rice. Nature. 2005;435(7045):1122–5. https://doi.org/10.1038/nature03630
  22. 21. Kim KY, Shin MS, Kim WJ, Mo YJ, Nam JK, Noh TH, et al. Effective combination of resistance genes against rice bacterial blight pathogen. Korean J Breed Sci. 2009;41(3):244–51.
  23. 22. Mishra D, Vishnupriya MR, Anil MG, Konda K, Raj Y, Sonti RV. Pathotype and genetic diversity amongst Indian isolates of Xanthomonas oryzae pv. oryzae. PLoS One. 2013;8(11):e81996. https://doi.org/10.1371/journal.pone.0081996
  24. 23. Indian Institute of Rice Research (ICAR-IIRR). All India Coordinated Rice Improvement Programme (AICRIP): Progress Report 2019–2020—Plant Pathology. Hyderabad: ICAR-IIRR; 2020. p. 3.63–3.84.

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