Gynostemium morphology and floral biology of Thottea duchartrei Sivar., A.Babu & Balach. (Aristolochiaceae), an endemic species from Western Ghats, India

Authors

DOI:

https://doi.org/10.14719/pst.2021.8.4.1315

Keywords:

Endemic species, Gynostemium, morphology, Staminode, Western Ghats, Thottea duchartrei

Abstract

Gynostemium is a putative floral structure formed by the fusion of the male and female reproductive parts (stamens and carpels) through a process during its development called synorganization. Most of the Indian species of Thottea Rottb. have a prominent gynostemium in their floral architecture, with varying degrees of adnation and complexity. Even though the gynostemium is a significant taxonomic marker for the inter-specific systematics in Thottea, the scepticism and arguments on deciphering its morphology and functional parameters, including the existence of staminodes, makes it imperative to be examined in detail. The present study is the first attempt in this line which aims to explore the morphological characters of gynostemium and reproductive biology of Thottea duchartrei Sivar., A.Babu and Balach., an endemic species in the Western Ghats. Stereo zoom microscopy, SEM and reproductive biological studies were conducted providing special emphasis to the gynostemium. Detailed analysis revealed the structural and functional diversity of gynostemium with regard to its components. The study also helped to recognize the columnar outgrowths on the gynostemium as staminodes. In total, the present study helps to solve the confusions regarding the functional identity of the gynostemium T. duchartrei with regard to its morphology.

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References

Endress PK. Development and evolution of extreme synorganization in angiosperm flowers and diversity: a comparison of Apocynaceae and Orchidaceae. Ann Bot (Oxford). 2016;117(5):749-67. https://doi.org/10.1093/aob/mcv119

Gonzalez F, Stevenson DW. Gynostemium development in Aristolochia (Aristolochiaceae). Bot Jahrb Syst. 2000;122(2):249-91.

Gonzalez F, Stevenson DW. Perianth development and systematics of Aristolochia. Flora. 2000;195(4):370-91. https://doi.org/10.1016/S0367-2530(17)30995-7

Rudall PJ, Bateman RM. Roles of synorganisation, zygomorphy and heterotopy in floral evolution: the gynostemium and labellum of orchids and other lilioid monocots. Biol Rev Cambridge Philos Soc. 2002;77(3):403-41. https://doi.org/10.1017/S1464793102005936

Wagstaff SJ, Wege J. Patterns of diversification in New Zealand Stylidiaceae. Amer J Bot. 2002;89(5):865-74. https://doi.org/10.3732/ajb.89.5.865

Yao TL. Aristolochiaceae. In: Kiew R, Chung RCK, Saw LG, Soepadmo E, (eds). Flora of Peninsular Malaysia, Series II: seed plants, vol 5. Kepong: Forest Research Institute Malaysia; 2015. p. 5-46.

Shaiju PN, Omanakumari N. Floral morphology and systematics of the genus Thottea Rottb. (Aristolochiaceae) from Western Ghats, India. Pl Syst Evol. 2010;288:213-25. https://doi.org/10.1007/s00606-010-0326-x

Murugan C. A review of the genus Thottea (Aristolochiaceae) from the Andaman and Nicobar Islands, including a new record for India. Rheedea. 2011;2:149-52.

Sunil CN, Kumar VVN. Thottea adichilthottiana (Aristolochiaceae), a new species from Ernakulam, Western Ghats, India. Webbia. 2014;69(2):239–42. https://doi.org/10.1080/00837792.2014.951205

Robi AJ, Jose PA, Udayan PS. Thottea sasidharaniana sp. nov. (Aristolochiaceae) from Peninsular India. Nordic Journal of Botany. 2014 Feb;32(1):11-14. https://doi.org/10.1111/j.1756-1051.2013.01577.x

Kumar ESS, Mathew SP, Jabbar MA, Krishnan SG, Murugesan, K. Rediscovery of Thottea dalzellii (Hook.f.) Karthik and Moorthy (Aristolochiaceae) from the Western Ghats. Int J Advanced Res. 2015;3(4):1-4.

Shaiju PN, Omanakumari N. Inflorescence morphology and systematics of the genus Thottea Rottb. (Aristolochiaceae) from the Western Ghats, India. Systematics and Biodiversity. 2009;7(4):445–51. https://doi.org/10.1017/S1477200009990181

Shaiju PN. Pollen morphology of the genus Thottea. Phytomorphology: An International Journal of Plant Morphology. 2012;62(3):125-35.

Shaiju PN, Omanakumari N. Chromosomal evolution in the genus Thottea Rottb. (Aristolochiaceae) from the Western Ghats, India. Nucleus. 2013;56(3):179-82. https://doi.org/10.1007/s13237-013-0095-3

Yigit N. Micromorphological Studies on Plants and Their Importance. In: Efe R, Matchavariani L, Yaldir A, Levai L. (eds). Developments in Science and Engineering. 2016. p.113-24.

Silva ALE, de Lima Terceiro LE, de Lima MF, Costa-Silva R, Dos Santos EAV, Agra MF. Leaf and stem micromorphology of Byrsonima sericea DC. by light and scanning electron microscopy. Microscopy Research and Technique. 2020;83(3):287-96. https://doi.org/10.1002/jemt.23412

Song JH, Yang S, Choi G. Taxonomic implications of leaf micromorphology using microscopic analysis: A tool for identification and authentication of Korean Piperales. Plants. 2020;9(5):566. https://doi.org/10.3390/plants9050566

Konarska A. Comparative micromorphology and anatomy of flowers and floral secretory structures in two Viburnum species. Protoplasma. 2017;254(1):523-37.https://doi.org/10.1007/s00709-016-0972-0

Giuliani CL, Foggi BR, Lippi MM. Floral morphology, micromorphology and palinology of selected Sedum s.l. species (Crassulaceae). Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 2018;152(3):1-16. https://doi.org/10.1080/11263504.2016.1271056

Lubna, Zafar M, Ahmad M, Shaheen S, Sultana S, Rehman SU, et al. Micromorphological investigation of leaf epidermis and seeds of Vitaceae from Pakistan using light microscopy and scanning electron microscopy. Microscopy Research and Technique. 2019;82(4):335-44.https://doi.org/10.1002/jemt.23102

Renuka C, Swarupanandan K. Morphology of the flower in Thottea siliquosa and the existence of staminodes in Aristolochiaceae. Blumea. 1986;31(2):313-18.

Leins P, Erbar C, Van Heel WA. Note on the floral development of Thottea (Aristolochiaceae). Blumea. 1988;33(2):357-70.

Heslop-Harrison J, Heslop-Harrison Y. Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate. Stain Technol. 1970;45(3):115-20. https://doi.org/10.3109/10520297009085351

Brewbaker JL, Kwack BH. The essential role of calcium ion in pollen germination and pollen tube growth. Amer J Bot. 1963;50(9):859-65. https://doi.org/10.1002/j.1537-2197.1963.tb06564.x

Shivanna KR, Tandon R. Reproductive Ecology of Flowering Plants: A Manual. New Delhi: Springer; 2014.p.42. https://doi.org/10.1007/978-81-322-2003-9

Kearns CA, Inouye DW. Techniques for the Pollination Biologists. Niwot: University press of Colorado; 1993.

Mattsson O, Knox RB, Heslop-Harrison J, Heslop-Harrison Y. Protein pellicle of stigmatic papillae as a probable recognition site in incompatibility reactions. Nature. 1974;247:298-300. https://doi.org/10.1038/247298a0

Sharma MV, Kuriakose G, Shivanna KR. Reproductive strategies of Strobilanthes kunthianus, an endemic, semelparous species in southern Western Ghats, India Bot J Linn Soc. 2008;157(1):155-63. https://doi.org/10.1111/j.1095-8339.2008.00786.x

Shivanna KR, Rangaswamy NS. Pollen Biology: a laboratory manual. Berlin: Springer; 1992. https://doi.org/10.1007/978-3-642-77306-8

Soltis PS, Brockington SF, Yoo MJ, Piedrahita A, Latvis M, Moore MJ, et al. Floral variation and floral genetics in basal angiosperms. Amer J Bot. 2009;96(1):110-28. https://doi.org/10.3732/ajb.0800182

Hou D. Florae Malesianae Praecursores LXII On the genus Thottea (Aristolochiaceae). Blumea. 1981;27(2):301-32.

Nazarudeen A, Sabu T. Staminal instability in Thottea duchartrei. Indian J Forest. 2002;25(2):194-95.

Shaiju PN. Systematics of the genus Thottea Rottb. (Aristolochiaceae) from the Western Ghats [Thesis], Kariavattom: University of Kerala; 2009.

Walker?Larsen J, Harder LD. The evolution of staminodes in angiosperms: patterns of stamen reduction, loss, and functional re?invention. Amer J Bot. 2000;87(10):1367-84. https://doi.org/10.2307/2656866

Dan M, Mathew PJ, Unnithan CM, Pushpangadan P. Thottea abrahamii a new species of Aristolochiaceae from Peninsular India. Kew Bull. 1996;51(1):179-82. https://doi.org/10.2307/4118756

Yao TL. Nine new species of Thottea (Aristolochiaceae) in Peninsular Malaysia and Singapore, with two taxa in Peninsular Malaysia redefined and a taxon lectotypified. Blumea. 2013;58(3):245–62. https://doi.org/10.3767/000651913X675791

Pandurangan AG, Nair VJ. Thottea idukkiana - a new species of Aristolochiaceae from Kerala, India. J Econ Taxon Bot. 1993;17(2):465-67.

Decraene LPR, Smets EF. Staminodes: their morphological and evolutionary significance. Bot Rev. 2001;67(3):351-402. https://doi.org/10.1007/BF02858099

Botnaru L, Schenk JJ. Staminode evolution in Mentzelia section Bartonia (Loasaceae) and its impact on insect visitation rates. Bot J Linn Soc. 2019;190:151-64. https://doi.org/10.1093/botlinnean/boz012

Sajo MG, Oriani A, Scatena VL, Rudall PJ. Floral ontogeny and vasculature in Xyridaceae, with particular reference to staminodes and stylar appendages. Pl Syst Evol. 2017;303:1293-310. https://doi.org/10.1007/s00606-017-1438-3

Meaders C, Min Y, Freedberg KJ, Kramer E. Developmental and molecular characterization of novel staminodes in Aquilegia. Ann Bot (Oxford). 2020;126(2):231-43. https://doi.org/10.1093/aob/mcaa029

Wang M, Huang S, Li M, McKey D, Zhang L. Staminodes influence pollen removal and deposition rates in nectar-rewarding self-incompatible Phanera yunnanensis (Caesalpinioideae). J Trop Ecol. 2019;35(1):34-42. https://doi.org/10.1017/S0266467418000433

Oelschlägel B, Wagner S, Salomo K, Pradeeep NS, Yao TL, Isnard S et al. Implications from molecular phylogenetic data for systematics, biogeography and growth form evolution of Thottea (Aristolochiaceae). Gard Bull Singapore. 2011;63(1&2):259-75.

McInnis SM, Emery DC, Porter R, Desikan R, Hancock JT, Hiscock SJ. The role of stigma peroxidases in flowering plants: insights from further characterization of a stigma-specific peroxidase (SSP) from Senecio squalidus (Asteraceae). J Exp Bot. 2006;57(8):1835-46. https://doi.org/10.1093/jxb/erj182

Kumar ESS, Chitra CR, Khan AES. Studies on the Trichomes of Indian Thottea Rottb. (Aristolochiaceae). J Econ Taxon Bot Addit Ser. 2001;19:237-40.

Edlund AF, Swanson R, Preuss D. Pollen and stigma structure and function: the role of diversity in pollination. The Plant Cell. 2004;16(1):S84-S97. https://doi.org/10.1105/tpc.015800

Zulkarnain Z, Eliyanti E, Swari EI. Pollen viability and stigma receptivity in Swainsona formosa (G. Don) J. Thompson (Fabaceae), an ornamental legume native to Australia. Ornam. Hortic. 2019;25(2):158-67. https://doi.org/10.14295/oh.v25i2.2011

Biswas P, Mohammad J. Studies on esterase and peroxidase activity of Clerodendrum inerme (Linn.) Gaertn. in relation to stigma receptivity. Int J Adv Res Biol Sci. 2016;6(3):386-89.

Femy KH, Radhamany PM, Gangaprasad A. Reproductive biology of Thottea barberi (Gamble) Ding Hou. (Aristolochiaceae) - an endemic taxon of southern Western Ghats, Kerala, India. Int J Plant Rep Biol. 2014;6(1):99-104.

Prychid CJ, Sokoloff DD, Remizowa MV, Tuckett RE, Yadav SR, Rudall PJ. Unique stigmatic hairs and pollen-tube growth within the stigmatic cell wall in the early-divergent angiosperm family Hydatellaceae. Ann Bot. (Oxford). 2011;108(4):599-608. https://doi.org/10.1093/aob/mcr021

Published

01-10-2021

How to Cite

1.
Sayoojia KS, Sreekala AK, Shaiju PN. Gynostemium morphology and floral biology of Thottea duchartrei Sivar., A.Babu & Balach. (Aristolochiaceae), an endemic species from Western Ghats, India. Plant Sci. Today [Internet]. 2021 Oct. 1 [cited 2024 Nov. 21];8(4):924–932. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1315

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Research Articles