In vitro antioxidant potential and antimicrobial activity of leaves and stem extracts of Anogeissus pendula Edgew.
DOI:
https://doi.org/10.14719/pst.2021.8.4.1341Keywords:
Traditional herbs, antioxidants, Phenolic compounds, antimicrobial activity, MIC, Minimum Inhibitory ConcentrationAbstract
Anogeissus pendula Edgew. is commonly used in the conventional Indian medicinal system and is reported to contain phenolic compounds which have antioxidant and antimicrobial potential. The goal of our study is to look at the antioxidant function and antibacterial activity of A. pendula leaf and stem extracts. The total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC) were determined using a spectrophotometric technique (TTC). In vitro techniques such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide radical scavenging tests (H2O2) and Ferric reducing antioxidant power (FRAP) assay were used in the study. The disc diffusion technique was used to assess antibacterial activity and the minimum inhibitory concentration (MIC) was investigated against four bacterial strains. The TTC of leaf and stem methanol extract was considerably higher which ranged from 15.07 ± 0.506 to 38.77 ± 1.253 mg gallic acid equivalent (GAE) /g in leaves and 19.83 ± 0.084 to 28.56 ± 0.437 mg GAE/g in the stem. The content of flavonoid in the leaf and stem methanol extract varied from 12.53 ± 0.603 to 37.28 ± 0.466 mg rutin equivalent (RE) /g in leaves and 10.01 ± 0.177 to 37.28 ± 0.466 mg RE/g in stems. Hydroalcoholic extract of leaf and stem showed the highest tannin content and ranged from 23.73 ± 0.091 to 34.08 ± 0.261 mg tannic acid equivalent (TAE) /g. In order of efficacy (IC50) of the plant extracts, the effective inhibitor was the methanol extract of leaf and stem in the DPPH and H2O2 assay. FRAP value was higher in the hydroalcoholic extract of both leaf and stem. Antimicrobial activity tests revealed that all extracts limit the development of diverse microbial strains such as Escherichia coli, Bacillus subtilis, Pseudomonas putida and Streptococcus aureus with a mean zone of inhibition ranging from 0 to 15.67 mm. The MIC of A. pendula leaf and stem solvent extracts against bacterial strains ranged from 0.195 to 50 mg/ml. The findings revealed that A. pendula has a variety of phytochemicals with substantial antioxidant and antibacterial properties, confirming its usage in traditional medicine.
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