Sodium fluoride (NaF) induced changes in growth and DNA profile of Vigna radiata
DOI:
https://doi.org/10.14719/pst.2016.3.1.137Keywords:
Fluoride, GTS, RAPD, Vigna radiataAbstract
Fluoride (F) is a natural, ubiquitous, non-biodegradable and hazardous pollutant. To investigate its effect, Vigna radiata seeds were treated with NaF (0, 25, 50, 75, 100 mg NaF kg-1) and its effect was studied on germination, growth and RAPD profile. The variation in DNA profile in response to NaF treatment was detected by RAPD-PCR technique. The result of RAPD was observed in terms of GTS% (Genomic Template Stability). Results showed that there was gradual decrease in GTS% i.e. increase in DNA damage from 25 to 75 mg NaF kg-1 concentration. These data demonstrate that RAPD is a reliable tool and permits greater insights into the genetic alteration of V. radiata.
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