Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes
DOI:
https://doi.org/10.14719/pst.1377Keywords:
biodiversity, sustainable agriculture, bio-inoculantAbstract
Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.
Downloads
References
Sparks D, Huang P. Physical chemistry of soil potassium. In: Munson RD (ed.), Potassium in agriculture, American Society of Agronomy, Medison. 1985; pp. 201-76. https://doi.org/10.2134/1985.potassium.c9
Meena VS, Maurya B, Verma JP. Does a rhizospheric microorganism enhance K+ availability in agricultural soils? Microbiol Res. 2014;169(5-6):337-47. https://doi.org/10.1016/j.micres.2013.09.003
Parmar P, Sindhu S. Potassium solubilization by rhizosphere bacteria: influence of nutritional and environmental conditions. J Microbiol Res. 2013;3(1):25-31. https://doi.org/10.5923/j.microbiology.20130301.04
Yadav AN, Kour D, Kaur T, Devi R, Yadav A, Dikilitas M, et al. Biodiversity, and biotechnological contribution of beneficial soil microbiomes for nutrient cycling, plant growth improvement and nutrient uptake. Biocatal Agric Biotechnol. 2021;33:102009. https://doi.org/10.1016/j.bcab.2021.102009
Archana D, Nandish M, Savalagi V, Alagawadi A. Screening of potassium solubilizing bacteria (KSB) for plant growth promotionalactivity. Bioinfolet. 2012;9(4):627-30.
Kaur T, Devi R, Kour D, Yadav A, Yadav AN, Dikilitas M, et al. Plant growth promoting soil microbiomes and their potential implications for agricultural and environmental sustainability. Biologia. 2021;76:2687–709. https://doi.org/10.1007/s11756-021-00806-w
Kour D, Rana KL, Yadav AN, Yadav N, Kumar M, Kumar V, et al. Microbial biofertilizers: Bioresources and eco-friendly technologies for agricultural and environmental sustainability. Biocatal Agric Biotechnol. 2020;23:101487. https://doi.org/10.1016/j.bcab.2019.101487
Yadav AN, Verma P, Kumar S, Kumar V, Kumar M, Sugitha TCK, et al. Actinobacteria from rhizosphere: molecular diversity, distributions, and potential biotechnological applications. In: Singh BP., Gupta VK., Passari AK (eds), New and Future Developments in Microbial Biotechnology and Bioengineering, Elsevier. 2018; pp. 13-41. https://doi.org/10.1016/B978-0-444-63994-3.00002-3
Chen Y, Rekha P, Arun A, Shen F, Lai W-A, Young C. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol. 2006;34(1):33-41. https://doi.org/10.1016/j.apsoil.2005.12.002
Sheng X-F, He L-Y, Huang W-Y. The conditions of releasing potassium by a silicate-dissolving bacterial strain NBT. Agric Sci China. 2002;1(6):662-6.
Gundala PB, Chinthala P, Sreenivasulu B. A new facultative alkaliphilic, potassium solubilizing, Bacillus sp. SVUNM9 isolated from mica cores of Nellore District, Andhra Pradesh, India. Research and Reviews. J Microbiol Biotechnol. 2013;2(1):1-7.
Sheng XF, He LY. Solubilization of potassium-bearing minerals by a wild-type strain of Bacillus edaphicus and its mutants and increased potassium uptake by wheat. Can J Microbiol. 2006;52(1):66-72. https://doi.org/10.1139/W05-117
Archana D, Nandish M, Savalagi V, Alagawadi A. Characterization of potassium solubilizing bacteria (KSB) from rhizosphere soil. Bioinfolet. 2013;10(1b):248-57.
Yadav AN, Kumar V, Prasad R, Saxena AK, Dhaliwal HS. Microbiome in Crops: Diversity, distribution and potential role in crops improvements. In: Prasad R., Gill SS., Tuteja N., (eds.), Crop Improvement through Microbial Biotechnology, Elsevier, USA. 2018; pp. 305-32. https://doi.org/10.1016/B978-0-444-63987-5.00015-3
Yadav AN, Mishra S, Singh S, Gupta A. Recent Advancement in White Biotechnology Through Fungi Volume 1: Diversity and Enzymes Perspectives. Springer International Publishing, Cham. 2019.
Yadav AN, Singh S, Mishra S, Gupta A. Recent Advancement in White Biotechnology Through Fungi. Volume 2: Perspective for Value-Added Products and Environments. Springer International Publishing, Cham. 2019.
Vyas P, Gulati A. Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonas. BMC Microbiol. 2009;9(1):174. https://doi.org/10.1186/1471-2180-9-174
Lian B, Fu P, Mo D, Liu C. A comprehensive review of the mechanism of potassium releasing by silicate bacteria. Acta Mineral Sin. 2002;22(2):179-83.
Hu X, Chen J, Guo J. Two Phosphate- and Potassium-solubilizing Bacteria Isolated from Tianmu Mountain, Zhejiang, China. World J Microbiol Biotechnol. 2006;22(9):983-90. https://doi.org/10.1007/s11274-006-9144-2
Pikovskaya R. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya. 1948;17:362-70.
Saravanan VS, Subramoniam SR, Raj SA. Assessing in vitro solubilization potential of different zinc solubilizing bacterial (zsb) isolates. Braz J Microbiol. 2004;35(1-2):121-5.
Yadav AN, Sachan SG, Verma P, Saxena AK. Bioprospecting of plant growth promoting psychrotrophic Bacilli from the cold desert of north western Indian Himalayas. Indian J Exp Biol. 2016;54:142-50.
Yadav AN, Sachan SG, Verma P, Tyagi SP, Kaushik R, Saxena AK. Culturable diversity and functional annotation of psychrotrophic bacteria from cold desert of Leh Ladakh (India). World J Microbiol Biotechnol. 2015;31(1):95-108. https://doi.org/10.1007/s11274-014-1768-z
Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24(8):1596-9. https://doi.org/10.1093/molbev/msm092
Lichtenthaler HK. [34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods Enzymol. 1987;148:350-82. https://doi.org/10.1016/0076-6879(87)48036-1
Irigoyen J, Einerich D, Sánchez?Díaz M. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant. 1992;84(1):55-60. https://doi.org/10.1111/j.1399-3054.1992.tb08764.x
Verma P, Yadav AN, Kazy SK, Saxena AK, Suman A. Elucidating the diversity and plant growth promoting attributes of wheat (Triticum aestivum) associated acidotolerant bacteria from southern hills zone of India. Natl J Life Sci. 2013;10(2):219-27.
Verma P, Yadav AN, Kazy SK, Saxena AK, Suman A. Evaluating the diversity and phylogeny of plant growth promoting bacteria associated with wheat (Triticum aestivum) growing in central zone of India. Int J Curr Microbiol Appl Sci. 2014;3(5):432-47.
Verma P, Yadav AN, Khannam KS, Panjiar N, Kumar S, Saxena AK, et al. Assessment of genetic diversity and plant growth promoting attributes of psychrotolerant bacteria allied with wheat (Triticum aestivum) from the northern hills zone of India. Ann Microbiol. 2015;65:1885-99. https://doi.org/10.1007/s13213-014-1027-4
Verma P, Yadav AN, Shukla L, Saxena AK, Suman A. Alleviation of cold stress in wheat seedlings by Bacillus amyloliquefaciens IARI-HHS2-30, an endophytic psychrotolerant K-solubilizing bacterium from NW Indian Himalayas. Natl J Life Sci. 2015;12(2):105-10.
Verma P, Yadav AN, Khannam KS, Kumar S, Saxena AK, Suman A. Molecular diversity and multifarious plant growth promoting attributes of Bacilli associated with wheat (Triticum aestivum L.) rhizosphere from six diverse agro-ecological zones of India. J Basic Microbiol. 2016;56(1):44-58. https://doi.org/10.1002/jobm.201500459
Saleem M, Asghar HN, Zahir ZA, Shahid M. Evaluation of lead tolerant plant growth promoting rhizobacteria for plant growth and phytoremediation in lead contamination. Rev Int Contam Ambient. 2019;35(4):999-1009. https://doi.org/10.20937/rica.2019.35.04.18
Heredia-Acuña C, Almaraz-Suarez JJ, Arteaga-Garibay R, Ferrera-Cerrato R, Pineda-Mendoza DY. Isolation, characterization and effect of plant-growth-promoting rhizobacteria on pine seedlings (Pinus pseudostrobus Lindl.). J For Res. 2019;30(5):1727-34. https://doi.org/10.1007/s11676-018-0723-5
Sangeeth K, Bhai RS, Srinivasan V. Paenibacillus glucanolyticus, a promising potassium solubilizing bacterium isolated from black pepper (Piper nigrum L.) rhizosphere. J Spices Aromat Crops. 2012;21(2):118-24.
Keshavarz Zarjani J, Aliasgharzad N, Oustan S, Emadi M, Ahmadi A. Isolation and characterization of potassium solubilizing bacteria in some Iranian soils. Arch Agron Soil Sci. 2013;59(12):1713-23. https://doi.org/10.1080/03650340.2012.756977
Zhang C, Kong F. Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants. Appl Soil Ecol. 2014;82:18-25. https://doi.org/10.1016/j.apsoil.2014.05.002
Subhashini D. Growth promotion and increased potassium uptake of tobacco by potassium-mobilizing bacterium Frateuria aurantia grown at different potassium levels in vertisols. Commun Soil Sci Plant Anal. 2015;46(2):210-20. https://doi.org/10.1080/00103624.2014.967860
Saha M, Maurya BR, Meena VS, Bahadur I, Kumar A. Identification and characterization of potassium solubilizing bacteria (KSB) from Indo-Gangetic Plains of India. Biocatal Agric Biotechnol. 2016;7:202-9. https://doi.org/10.1016/j.bcab.2016.06.007
Bakhshandeh E, Pirdashti H, Lendeh KS. Phosphate and potassium-solubilizing bacteria effect on the growth of rice. Ecol Eng. 2017;103:164-9. https://doi.org/10.1016/j.ecoleng.2017.03.008
Meena VS, Zaid A, Maurya BR, Meena SK, Bahadur I, Saha M, et al. Evaluation of potassium solubilizing rhizobacteria (KSR): enhancing K-bioavailability and optimizing K-fertilization of maize plants under Indo-Gangetic Plains of India. Environ Sci Pollut Resh. 2018;25(36):36412-24. https://doi.org/10.1007/s11356-018-3571-0
Sun F, Ou Q, Wang N, Guo Zx, Ou Y, Li N, et al. Isolation and identification of potassium-solubilizing bacteria from Mikania micrantha rhizospheric soil and their effect on M. micrantha plants. Global Ecol Conserv. 2020;23:e01141. https://doi.org/10.1016/j.apsoil.2014.05.002
Pramanik P, Goswami AJ, Ghosh S, Kalita C. An indigenous strain of potassium?solubilizing bacteria Bacillus pseudomycoides enhanced potassium uptake in tea plants by increasing potassium availability in the mica waste?treated soil of North?east India. J Appl Microbiol. 2019;126(1):215-22. https://doi.org/10.1111/jam.14130
Ashfaq M, Hassan HM, Ghazali AHA, Ahmad M. Halotolerant potassium solubilizing plant growth promoting rhizobacteria may improve potassium availability under saline conditions. Environ Monit Assess. 2020;192(11):697. https://doi.org/10.1007/s10661-020-08655-x
Suyal DC, Joshi D, Kumar S, Bhatt P, Narayan A, Giri K, et al. Himalayan Microbiomes for Agro-Environmental Sustainability: Current Perspectives and Future Challenges. Microb Ecol. 2021. https://doi.org/10.1007/s00248-021-01849-x
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Tanvir Kaur, Rubee Devi, Divjot Kour, Ashok Yadav, Ajar Nath Yadav
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
