Yield and morpho-physiological traits of tobacco (Nicotiana tabacum L.) as affected by azotobacter, mycorrhizal symbiosis and biochar application

Authors

DOI:

https://doi.org/10.14719/pst.1378

Keywords:

biochar, Biofertilizer, yield, tobacco, rainfed

Abstract

The present study was performed to evaluate the effect of mycorrhizal symbiosis and azotobacter with the application of biochar on tobacco growth parameters, yield and nicotine content under rainfed conditions for two growing seasons (2016-2017 and 2017-2018). The factorial experiment was performed based on a randomized complete block design with four replications and three factors including biochar at three levels (0, 4 and 8 ton/ha), mycorrhizal fungi and Azotobacter chroococcum each one at two levels (without and with application). Results showed that biochar application had a significant and positive effect on all evaluated parameters. However, there was no statistically significant difference between 4 and 8 tons per hectare of biochar in yield. Mycorrhizal symbiosis had a significant effect on relative water content. Application of azotobacter had a positive and significant effect on growth parameters, yield and nicotine content under rainfed conditions for two growing seasons (2016-2017 and nicotine content. The greatest effect of azotobacter with a 17% increase was on leaf nicotine content. Whereas tobacco dry yield in rainfed conditions is lower, the combined use of biochar and these biofertilizers can be considered a desirable solution. Then in terms of economic aspects, the use of 4 tons biochar per hectare along with the use of mycorrhiza and azotobacter to achieve acceptable yield while maintaining chemical quality in tobacco farms is recommended.

Downloads

Download data is not yet available.

References

Ganapathi TR, Suprasanna P, Roa PS, Bapat VA. Tobacco (Nicotiana tabacum L.) Amodel system for tissue culture interventions and genetic engineering. Indian J. of Biotechnology; 2004. 3:171–184.

Tang Z, Chen L, Chen Z, Fu Y, Sun X, et al. Climatic factors determine the yield and quality of Honghe flue cured tobacco. Scientifc Reports; 2020. 10, Article number: 19868 https://doi.org/10.1038/s41598-020-76919-0

Soliman MH, Abdulmajeed AM, Alhaithloul H, Alharbi BM, El-Esawi MA, et al. Saponin Biopriming Positively Stimulates Antioxidants Defense, Osmolytes Metabolism and Ionic Status to Confer Salt Stress Tolerance in Soybean. Acta Physiol. Plant; 2020. 42: 114. https://doi.org/10.1007/s11738-020-03098-w

Major J, Rondon M, Molina D, Riha SJ, Lehmann J. Maize yield and nutrition during 4 years after biochar application to a Colombian savanna oxisol. Plant Soil; 2010. 333: 117–128. https://doi.org/10.1007/s11104-010-0327-0

El Nahhas N; AlKahtani MDF, Abdelaal KAA, Al Husnain L, AlGwaiz HIM, et al. Biochar and Jasmonic Acid Application Attenuates Antioxidative Systems and Improves Growth, Physiology, Nutrient Uptake and Productivity of Faba Bean (Vicia Faba L.) Irrigated with Saline Water. Plant Physiol. Biochem; 2021. 166: 807–817. https://doi.org/10.1016/j.plaphy.2021.06.033

Abbas T, Rizwan M, Ali S, Rehman MZ, Qayyum MF, Abbas F, et al. Effect of biochar on cadmium bioavailability and uptake in wheat (Triticum aestivum L.) grown in a soil with aged contamination. Ecotoxicol Environ Saf; 2017. 140:37–47. https://doi.org/10.1016/j.ecoenv.2017.02.028.

Moosavi SA, Shokuhfar A, Lak S, Mojaddam M, Alavifazel M. Integrated application of biochar and bio-fertilizer improves yield and yield components of cowpea under water-deficient stress. Ital. J. Agron; 2020. 15: 94–101. https://doi.org/10.4081/ija.2020.1581.

Jaafar NM, Clode PL, Abbott LK. Soil microbial responses to biochars varying in particle size, surface and pore properties. Pedosphere; 2015. 25:770–780. https://doi.org/10.1016/S1002-0160(15)30058-8.

Kim HS, Kim KR, Yang JE, Ok YS, Owens G, et al. Effect of biochar on reclaimed tidal land soil properties and maize (Zea mays L.) response. Chemosphere; 2016. 142:153–159. https://doi.org/10.1016/j.chemosphere.2015.06.041.

Lim TJ, Spokas KA, Feyereisen G, Novak JM. Predicting the impact of biochar additions on soil hydraulic properties. Chemosphere; 2016. 142:136–144. https://doi.org/10.1016/j.chemosphere.2015.06.069

Ojeda G, Mattana S, Àvila A, Alcañiz JM, Volkmann M, et al. Are soil-water functions affected by biochar application? Geoderma; 2015. 249: 1–11. https://doi.org/10.1016/j.geoderma.2015.02.014.

Jiangzhou L, Sigui J, Limeng Z, Qingzhong Z. Effects of biochar on soil quality and tobacco growth during four years of consecutive application. Institute of Environmental and Sustainable Development in Agriculture. Chinese Agricultural Science; 2016. APPOST 16.

Shokri S, Maadi B. Effects of arbuscular mycorrhizal fungus on the mineral nutrition and yield of Trifolium alexandrinum plants under salinity stress. J. Agron; 2009. 8: 79–83. https://doi.org/10.3923/ja.2009.79.83

Roesti D, Gaur R, Johri B, Imfeld G, Sharma S, et al. Plant growth stage, fertilizer management and bio-inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields. Soil Biol. Biochem; 2006. 38: 1111–1120. https://doi.org/10.1016/j.soilbio.2005.09.010

Mickan BS, Abbott LK, Stefanova K, Solaiman ZM. Interactions between biochar and mycorrhizal fungi in a water-stressed agricultural soil. Mycorrhiza; 2016. 26: 565–574. https:// 10.1007/s00572-016-0693-4.

Aquilanti L, Favilli F, Clementi F. Comparison of different strategies for isolation and preliminary identification of azotobacter from soil samples. Soil Biol; 2004. 36(9): 1475–1483. https://doi.org/10.1016/j.soilbio.2004.04.024

Sandhya V, Ali SKZ, Grover M, Reddy G, Enkateswarlu BV. Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress. Plant Growth Regul; 2010. 62: 21–30. https://doi.org/10.1007/s10725-010-9479-4.

Shabani G, Ardakani MR, Chaichi MR, Friedel JK, Khavazi K. Effect of different fertilizing treatments on nutrient uptake in annual medic (Medicago scutellata cv. robinson) under irrigated and dry farming systems. J. Agr. Sci. Tech; 2015. 17: 299–310. http://jast.modares.ac.ir/article-23-10126-en.html.

Abbasi MK, Sharif S, Kazmi M, Sultan T, Aslam M. Isolation of plant growth promoting rhizobacteria from wheat rhizosphere and their effect on improving growth, yield and nutrient uptake of plants. Plant Biosystems; 2011. 145(1) :159–168. https://doi.org/10.1080/11263504.2010.542318

Zaidi A, Khan MS, M Ahemad M, Oves M. Plant growth promotion by phosphate solubilizing bacteria. Acta Microbiologica et Immunologica Hungarica; 2009. 56(3):263–284. https://doi.org/10.1556/AMicr.56.2009.3.6

Subhashini DV. Effect of bio-inoculation of AM fungi and PGPR on the growth, yield and quality of FCV tobacco (Nicotiana tabacum) in vertisols. Ind. J. Agric. Sci; 2013. 83 (6): 667?672.

Ruscitti M, Arango M, Ronco M, Beltrano J. Inoculation with mycorrhizal fungi modifies proline metabolism and increases chromium tolerance in pepper plants (Capsicum annuum L.). Braz. J. Plant Physiol; 2011. 23(1): 15–25.

Jnawali AD, Ojha RB, Marahatta S. Role of azotobacter in soil fertility and sustainability–a review. Adv Plants Agric Res; 2015. 2(6): 250?253. https://doi.org/10.15406/apar.2015.02.00069

Smart RE, Bingham GE. Rapid estimates of relative water content. Plant Physiol; 1974. 53: 258–260. https://doi.org/10.1104/pp.53.2.258.

Phillips JM, Hayman DS. Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society; 1970. 55: 158–161.

Giovannetti M, Mosse B. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol; 1980. 84(3): 489–500. doi:10.1111/j.1469-8137.1980.tb04556.x

CORESTA recommended method No 35. Determination of total alkaloids (as nicotine) in tobacco by continuous flow analysis; 1994. Available from: https://www.coresta.org/sites/default/files/technical_documents/main/CRM_35-update Aug10).pdf

Copetta A, Lingua G, Berta G. Effects of three AM fungi on growth, distribution of glandular hairs and essential oil production in Ocimum basilicum L. Mycorrhiza; 2006. 16: 485–494. https://doi.org/10.1007/s00572-006-0065-6.

- Park, Y-S, Park K, Kloepper JW, Ryu C-M. Plant Growth-Promoting Rhizobacteria stimulate vegetative growth and asexual reproduction of Kalanchoe daigremontiana. Plant Pathol. J; 2015. 31: 310–315. https://doi.org/10.5423/PPJ.NT.01.2015.0006.

Adiprasetyo T, Purnomo B, Handajaningsih M, Hidayat H. The usage of BIOM3G-Biofertilizer to improve and support sustainability of land system of independent oil palm smallholders. Int. J. Adv. Sci. Eng. Inf. Technol; 2014. 4: 345–348. http://dx.doi.org/10.18517/ijaseit.4.5.431

Javan Gholiloo M, Yarnia M, Ghorttapeh AH, Farahvash F, Daneshian AM. Evaluating e?ects of drought stress and bio-fertilizer on quantitative and qualitative traits of valerian (Valeriana o?cinalis L.). J. Plant Nutr.; 2019. 42: 1417–1429. https://doi.org/10.1080/01904167.2019.1628972

Nadeem SM, Ahmad M, Zahir ZA, Javaid A, Ashraf M. The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments. Biotechnol. Adv.; 2014. 32: 429–448. https://doi.org/10.1016/j.biotechadv.2013.12.005

Raklami A, Bechtaoui N, Tahiri A, Anli M, Meddich A, et al. Use of rhizobacteria and mycorrhizae consortium in the open ?eld as a strategy for improving crop nutrition, productivity and soil fertility. Front. Microbiol; 2019. 10:1106. https://doi.org/10.3389/fmicb.2019.01106

Yu YY, Li SM, Qiu JP, Li JG, Luo YM, et al. Combination of agricultural waste compost and biofertilizer improves yield and enhances the sustainability of a pepper ?eld. J. Plant Nutr. Soil Sci; 2019. 182: 560–569. https://doi.org/10.1002/jpln.201800223

Grobelak A, Napora A, Kacprzak M. Using plant growth-promoting rhizobacteria (PGPR) to improve plant growth. Ecol. Eng; 2015. 84: 22–28. https://doi.org/10. 1016/j.ecoleng.2015.07.019

Nigussie A, Kissi E, Misganaw M, Ambaw G. Effect of biochar application on soil properties and nutrient uptake of Lettuces (Lactuca sativa) grown in chromium polluted soils. American-Eurasian Journal of Agriculture and Environmental Science; 2012. 12(3): 369-376.

Chan K, Van Zwieten L, Meszaros I, Downie A, Joseph S. Using poultry litter biochars as soil amendments. Australian Journal of Soil Research; 2008. 46(5): 437?444.

Saxena J, Rana G, Pandey M. Impact of addition of biochar along with Bacillus sp. on growth and yield of French beans. Scientia Horticulturae; 2013. 162: 351?356. https://doi.org/10.1016/J.SCIENTA.2013.08.002

Placek A, Grobelak A, Kacprzak M. Improving the phytoremediation of heavy metals contaminated soil by use of sewage sludge. International Journal of Phytoremediation; 2016. 18(6): 605?618. https://doi.org/10.1080/15226514.2015.1086308.

Agegnehu G, Srivastava A, Bird MI. The role of biochar and biochar-compost in improving soil quality and crop performance: a review. Appl. Soil Ecol; 2017. 119: 156–170. https://doi.org/10.1016/j.apsoil.2017.06.008.

Suliman W, Harsh JB, Abu-Lail NI, Fortun AM, Dallmeyer I, GarciaPérez M. The role of biochar porosity and surface functionality in augmenting hydrologic properties of a sandy soil. Sci Total Environ; 2017. 574: 139–147. https://doi.org/10.1016/j.scitotenv.2016.09.025.

Tayyab M, Islam W, Khalil F, Ziqin P, Caifang Z, Arafat Y, et al. Biochar: an efficient way to manage low water availability in plants. Appl Ecol Environ Res; 2018. 16: 2565?2583. https:// 10.15666/aeer/1603_25652583.

Behrooz A, Vahdati K. Arbuscular mycorrhiza and plant growth-promoting bacteria alleviate drought stress in Walnut. J Am Soc Hortic Sci; 2019. 54: 1087?1092. https://doi.org/10.21273/HORTSCI13961-19.

Rapparini F, Penuelas J. Mycorrhizal fungi to alleviate drought stress on plant growth, In: M. Miransari (ed.). Use of microbes for the alleviation of soil stresses. Vol. 1. Springer, New York, NY; 2014. 1: 21?42. https://10.1007/978-1-4614-9466-9_2.

- Castiglione AM, Mannino G, Contartese V, Bertea CM, Ertani, A. Microbial Biostimulants as Response to Modern Agriculture Needs: Composition, Role and Application of These Innovative Products. Plants; 2021. 10: 1533. https://doi.org/10.3390/ plants10081533

Downie A, Crosky A, Munroe P. Physical properties of biochar. In: J. Lehmann and S. Joseph (Eds.). Biochar for Environmental Management: Science and Technology. 3rd Ed, London, Earthscan; 2009. 405 p.

Glaser B, Lehmann J, Zech W. Ameliorating physical and chemical properties of highly weathered soils in the tropics with charcoal a review. Biol Fertil Soils; 2002. 35(4): 219?230. https://doi.org/10.1007/s00374-002-0466-4.

Beltrano J Ronco MG. Improved tolerance of wheat plants (Triticum aestivum L.) to drought stress And rewatering by the arbuscular mycorrhizal fungus Glomus claroideum: effect on growth and cell Membrane stability, Braz. J. Plant Physiol; 2008. 20: 29?37. https://doi.org/10.1590/S1677-04202008000100004.

Meddich A, Oihabi A, Abbas Y, Essia B. Rôle des champignons mycorhiziens à arbuscules de zones arides dans la résistance du trèfle (Trifolium alexandrinum L.) au déficit hydrique au de?cit hydrique, Agronomic; 2000. 20: 283?295. https://doi.org/10.1051/agro:2000127.

Aasfar A, Bargaz A, Yaakoubi K, Abderraouf Hilali A, Bennis I, Youssef Zeroual Y, et al. Nitrogen Fixing Azotobacter Species as Potential Soil Biological Enhancers for Crop Nutrition and Yield Stability, 2021. Front. Microbiol. 12:628379. https://doi.org/10.3389/fmicb.2021.628379

Lawlor DW, Cornic G. Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environ; 2002. 25(2):275?294. https://doi.org/10.1046/j.0016-8025.2001.00814.x.

Selosse MA, Strullu-Derrien C, Martin FM, Kamoun S, Kenrick P. Plants, fungi and oomycetes: a 400-million years’ affair that shapes the biosphere. New Phytol; 2015. 206: 501?506. https://doi.org/10.1111/nph.13371.

Davaran Hagh E, Mirshekari B, Ardakani MR, Farahvash F, Rejali F. Optimizing phosphorus use in sustainable maize cropping via mycorrhizal inoculation. J. Plant Nutr; 2016. 39: 1348?1359. https://doi.org/10.1080/01904167.2015.1086797.

Van der Heijden MG, Martin FM, Selosse MA, Sanders IR. Mycorrhizal ecology and evolution: the past, the present, and the future. New Phytol; 2015. 205: 1406?1423. https://doi.org/10.1111/nph.13288.

Warnock DD, Lehmann J, Kuyper TW, Rillig MC. Mycorrhizal responses to biochar in soil concepts and mechanisms. Plant Soil; 2007. 300: 9?20. https://doi.org/10.1007/s11104-007-9391-5.

Hammer EC, Forstreuter M, Rillig MC, Kohler J. Biochar increases arbuscular mycorrhizal plant growth enhancement and ameliorates salinity stress. Appl Soil Ecol; 2015. 96: 114?121. https://doi.org/10.1016/j.apsoil.2015.07.014.

Hammer EC, Balogh-Brunstad Z, Jakobsen I, Olsson PA, Stipp SLS, Rilling MC. A mycorrhizal fungus grows on biochar and captures phosphorus from its surfaces. Soil Biol Biochem; 2014. 77: 252-260. https://doi.org/10.1016/j.soilbio.2014.06.012.

Shang X, Shang Y, Fu J, Zhan T. Nicotine significantly improves chronic stress-induced impairments of cognition and synaptic plasticity in mice. Mol Neurobiol; 2017. 54: 4644?4658. https://doi.org/10.1007/s12035-016-0012-2.

Baldwin IT. An ecologically motivated analysis of plant herbivore interactions in native tobacco. Plant Physiol; 2001. 127: 1449–1458. https://doi.org/10.1104/pp.010762.

Fritz C, Palacios-Rojas N, Feil R, Still M. Regulation of secondary metabolism by the Carbon-nitrogen status in tobacco: nitrate inhibits large sectors of phenylpropanoid metabolism, 2006. Plant J; 2006. 46: 533?548. https://doi: 10.1111/j.1365-313X.2006.02715. x.

Mardukhi B, Rejali F, Daei G, Ardakani MR, Malakouti MJ, Miransari M. Mineral uptake of mycorrhizal wheat (Triticum aestivum L.) under salinity stress. Commun. Soil Sci Plant Anal; 2015. 46, 343?357. https://doi.org/10.1080/00103624.2014.981271.

Published

13-11-2021

How to Cite

1.
Mesbah R, Ardakani MR, Moghaddam A, Rafiei F. Yield and morpho-physiological traits of tobacco (Nicotiana tabacum L.) as affected by azotobacter, mycorrhizal symbiosis and biochar application. Plant Sci. Today [Internet]. 2021 Nov. 13 [cited 2024 Nov. 4];8(4):986–994. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1378

Issue

Section

Research Articles

Most read articles by the same author(s)