Exogenous foliar application of abscisic acid on polyethylene glycol induced drought by improving the morphological and biochemical characters of four rice (Oryza sativa L.) varieties
DOI:
https://doi.org/10.14719/pst.1396Keywords:
Abscisic acid, Antioxidant, Biochemical analysis, Drought, Oryza sativa, PEGAbstract
Climate change is one of the critical defining concerns today’s world, altering earth ecosystem. Drought stress management is a major issue in agricultural and crop research, mainly negatively impacting rice growth and yield. Abscisic acid (ABA) is a plant stress hormone that plays a crucial role in regulating, and mitigating drought stress. The objective of this study was to evaluate the effect of exogenously applied ABA and adaptation strategies responding to concrete challenges four rice varieties (ASD-16, ADT-45, TKM-13 and CO-50) and analysed growth characteristics, physiological, biochemical and antioxidative enzyme activities induced by polyethylene glycol (2 % and 4 %) drought stress and exogenously application of ABA (100 µM). The present findings help predict the degree of drought resistance variety of rice. The experiment was designed by six different treatments, such as control plants, control + ABA, polyethylene glycol induced drought (2 % and 4 %), and combination treatment of 2 % and 4 % PEG with exogenous application of ABA (100 µM). Exogenous ABA treatment significantly increased in morphological characteristics compared to control. The chlorophyll pigments, RWC, biochemical parameters such as reducing sugar, starch, protein and antioxidant activities of CAT, POD were increased and proline content was decreased at PEG 2 % and 100 µM ABA-treated rice in TKM-13 compared to ASD-16, ADT-45, CO-50. There were statistically significant morphological, physiological and biochemical parameters between treatments. The present findings depict that four rice varieties under drought imposition decrease the growth characteristics, physiological and biochemical content. However, the PEG induced drought (2 %) and foliar application of ABA (100 µM) were found to increase morphology, physiological and biochemical contents and can provide valuable insights into plants’ drought responses and may help identify novel drought tolerance traits.
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References
Desa U. United Nations, Department of Economic and Social Affairs, Population Division. World Population Prospects (UNDESAPD). 2019.
Ullah H, Santiago-Arenas R, Ferdous Z, Attia A, Datta A. Improving water use efficiency, nitrogen use efficiency and radiation use efficiency in field crops under drought stress: A review. Advances in Agronomy. 2019 Jan 1; 156:109-57. https://doi.org/10.1016/bs.agron.2019.02.002
FAO. The future of food and agriculture—Alternative pathways to 2050, Rome. 2018.
Raina A, Khan S, Sahu PK, Sao R. Increasing Rice Grain Yield Under Abiotic Stresses: Mutagenesis, Genomics and Transgenic Approaches. In Rice Research for Quality Improvement: Genomics and Genetic Engineering. Springer, Singapore. 2020; pp.753-77. https://doi.org/10.1007/978-981-15-4120-9_31
Basal O, Szabo A, Veres S. PEG-induced drought stress effects on soybean germination parameters. Journal of Plant Nutrition. 2020 Jul 20; 43(12):1768-79. https://doi.org/10.1080/01904167.2020.1750638
Awan SA, Khan I, Rizwan M, Zhang X, Brestic M, Khan A, El-Sheikh MA, Alyemeni MN, Ali S, Huang L. Exogenous abscisic acid and jasmonic acid restrain PEG-induced drought by improving the growth and antioxidative enzyme activities in pearl millet. Physiologia Plantarum. 2020 Oct 22. https://doi.org/10.1111/ppl.13247
Wheeler T, Von Braun J. Climate change impacts on global food security. Science. 2013 Aug 2; 341(6145):508-13. https://doi.org/10.1126/science.1239402
Wang J, Chen J, Pan K. Effect of exogenous abscisic acid on the level of antioxidants in Atractylodes macrocephala Koidz under lead stress. Environmental Science and Pollution Research. 2013 Mar; 20(3):1441-49. https://doi.org/10.1007/s11356-012-1048-0
Finkelstein R. Abscisic acid synthesis and response. The Arabidopsis book/American Society of Plant Biologists. 2013; 11. https://doi.org/10.1199/tab.0166
Ramachandran M, Arulbalachandran D, Jothimani K. ABA-Mediated drought stress resistance in crops for sustainable agriculture. Sustainable Agriculture Towards Food Security. 2017; 69-83. https://doi.org/10.1007/978-981-10-6647-4_5
Du H, Wu N, Fu J, Wang S, Li X, Xiao J, Xiong L. A GH3 family member, OsGH3-2, modulates auxin and abscisic acid levels and differentially affects drought and cold tolerance in rice. Journal of Experimental Botany. 2012 Nov 1; 63(18):6467-80. https://doi.org/10.1093/jxb/ers300
Chen J, Shiyab S, Han FX, Monts DL, Waggoner CA, Yang Z, Su Y. Bioaccumulation and physiological effects of mercury in Pteris vittata and Nephrolepis exaltata. Ecotoxicology. 2009 Jan; 18(1):110-21. https://doi.org/10.1007/s10646-008-0264-3
Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology. 1949 Jan; 24(1):1. https://doi.org/10.1104/pp.24.1.1
Kirk JTO, Allen RL. Dependence of chloroplast pigments synthesis an protein synthetic effects of acitilione. Biochemical Biophysics Research Communication. 1965 Dec 21; 21(6):523-30. https://doi.org/10.1016/0006-291X(65)90516-4
Nelson N. A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry. 1944 May 1; 153(2):375-80. https://doi.org/10.1016/S0021-9258(18)71980-7
Cleeg KM. The application of the anthrone reagent to the estimation of starch in cereal. J. Sci. Food Agric. 1956; 7:40-45. https://doi.org/10.1002/jsfa.2740070108
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 1951; 193:265-75. https://doi.org/10.1016/S0021-9258(19)52451-6
Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant and Soil. 1973 Aug; 39(1):205-57. https://doi.org/10.1007/BF00018060
Chandlee JM, Scandalios JG. Analysis of variants affecting the catalase developmental program in maize scutellum. Theoretical and Applied Genetics. 1984 Nov; 69(1):71-7. https://doi.org/10.1007/BF00262543
Maehly A, Chance B. Catalases and eroxidise. Methods Biochem Anal. 01 Jan 1954; 1:357-424. https://doi.org/10.1002/9780470110171.ch14
Sankar B, Gopinathan P, Karthishwaran K, Somasundaram R. Variation in growth of peanut plants under drought stress condition and in combination with paclobutrazol and ABA. Current Botany. 2016 Jan 5; 5:14-21.
Zhang J, Jia W, Yang J, Ismail AM. Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Research. 2006 May 5; 97(1):111-19. https://doi.org/10.1016/j.fcr.2005.08.018
Naz H, Khan N. Role of abscisic acid and water stress on the activities of antioxidant enzymes in wheat. Current Research Journal of Biological Sciences. 2014 Jul 20; 6(4):168-72. https://doi.org/10.19026/crjbs.6.5516
Teulat B, Zoumarou-Wallis N, Rotter B, Salem MB, Bahri H, This D. QTL for relative water content in field-grown barley and their stability across Mediterranean environments. Theoretical and Applied Genetics. 2003 Dec; 108(1):181-88. https://doi.org/10.1007/s00122-003-1417-7
Endo A, Sawada Y, Takahashi H, Okamoto M, Ikegami K, Koiwai H, Seo M, Toyomasu T, Mitsuhashi W, Shinozaki K, Nakazono M. Drought induction of Arabidopsis 9-cis-epoxycarotenoid dioxygenase occurs in vascular parenchyma cells. Plant Physiology. 2008 Aug; 147(4):1984-93. https://doi.org/10.1104/pp.108.116632
Ploenlap P, Pattanagul W. Effects of exogenous abscisic acid on foliar anthocyanin accumulation and drought tolerance in purple rice. Biologia. 2015 Jul; 70(7):915-21. https://doi.org/10.1515/biolog-2015-0107
Gadallah MA. Effect of water stress, abscisic acid and proline on cotton plants. Journal of arid environments. 1995 Jul 1; 30(3):315-25. https://doi.org/10.1016/S0140-1963(05)80006-0
Pattanagul W. Exogenous abscisic acid enhances sugar accumulation in rice (Oryza sativa L.) under drought stress. Asian Journal of Plant Sciences. 2011; 10(3):212-19. https://doi.org/10.3923/ajps.2011.212.219
Lee BR, Jin YL, Jung WJ, Avice JC, Morvan?Bertrand A, Ourry A, Park CW, Kim TH. Water?deficit accumulates sugars by starch degradation—not by de novo synthesis—in white clover leaves (Trifolium repens). Physiologia Plantarum. 2008 Nov;134(3):403-11. https://doi.org/10.1111/j.1399-3054.2008.01156.x
Yoshida T, Mogami J, Yamaguchi-Shinozaki K. Omics approaches toward defining the comprehensive abscisic acid eroxidise network in plants. Plant and Cell Physiology. 2015 Jun 1; 56(6):1043-52. https://doi.org/10.1093/pcp/pcv060
Finkelstein RR, Gibson SI. ABA and sugar interactions regulating development: cross-talk or voices in a crowd?. Current Opinion in Plant Biology. 2002 Feb 1; 5(1):26-32. https://doi.org/10.1016/S1369-5266(01)00225-4
Maleki M, Ebrahimzade H, Gholami M, Niknam V. The effect of drought stress and exogenous abscisic acid on growth, protein content and antioxidative enzyme activity in saffron (Crocus sativus L.). African Journal of Biotechnology. 2011; 10(45):9068-75. https://doi.org/10.5897/AJB10.676
Zhou L, Xu H, Mischke S, Meinhardt LW, Zhang D, Zhu X, Li X, Fang W. Exogenous abscisic acid significantly affects proteome in tea plant (Camellia sinensis) exposed to drought stress. Horticulture Research. 2014 Jun 25; 1(1):1-9. https://doi.org/10.1038/hortres.2014.29
Bray EA. Abscisic acid regulation of gene expression during water?deficit stress in the era of the Arabidopsis genome. Plant, cell and environment. 2002 Feb; 25(2):153-61. https://doi.org/10.1046/j.1365-3040.2002.00746.x
Unyayar S, Keleþ Y, Unal E. Proline and ABA levels in two sunflower genotypes subjected to water stress. InBulg J Plant Physiol. 2004.
Vajrabhaya M, Kumpun W, Chadchawan S. The solute accumulation: The mechanism for drought tolerance in RD23 rice (Oryza sativa L.) lines. Sci. Asia. 2001 Jun; 27:93-97. https://doi.org/10.2306/scienceasia1513-1874.2001.27.093
Saeedipour S. Relationship of grain yield, ABA and proline accumulation in tolerant and sensitive wheat cultivars as affected by water stress. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2013 Sep; 83(3):311-15. https://doi.org/10.1007/s40011-012-0147-5
Ramachandran M, Arulbalachandran D, Dilipan E, Ramya S. Comparative analysis of abscisic acid recovery on two varieties of rice (Oryza sativa L.) under drought condition. Biocatalysis and Agricultural Biotechnology. 2021 May 1; 33:102006. https://doi.org/10.1016/j.bcab.2021.102006
Arulbalachandran D, Yasmin K, Jothimani K, Ramachandran M, Pradeepkumar R. Role of ABA on antioxidant mechanism under drought crops. International Journal for Species. 2016; 17:48-55.
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