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Research Articles

Early Access

Effect of poultry manure and spraying with urea on growth and yield parameters of local lettuce

DOI
https://doi.org/10.14719/pst.14186
Submitted
21 February 2026
Published
14-04-2026

Abstract

This study aims to study the effect of the interaction between poultry manure levels and foliar urea concentrations in improving the growth and productivity of the lettuce plant. A field experiment was undertaken during the 2024–2025 winter growing season to investigate the impact of varying poultry manure levels and urea foliar spray on local lettuce growth and productivity. The experiment was conducted in the College of Agriculture, University of Anbar, Iraq, at roughly 33.35° N latitude and 43.79° E longitude. Three poultry manure levels (0, 15 and 30 t ha-1) and 3 foliar urea concentrations (0, 0.5 and 1 %) were tested. The experiment employed 3 replications as a randomized complete block design (RBD). Application of 30 t ha-1 poultry manure significantly increased plant height, total number of leaves, leaf area, plant weight and total yield, with the highest yield of 58.32 t ha-1. As for the effect of spraying with urea, the U2 treatment showed the best values for the studied traits including (plant height, total number of leaves, leaf thickness, leaves area, head diameter, total plant weight and total yield) which reached (42.03 cm, 64.17 leaf plant-1, 0.32 mm, 35.94 dm² plant-1, 20.54 cm, 1.68 kg and 55.69 t ha-1), respectively. On the other hand, the overlap of the 2 study factors revealed a significant effect on all examined parameters excluding leaf thickness and the best values for all traits appeared when W2U2 was treated, while the treatment of no addition of poultry manure and spraying with urea W0U0 showed the lowest values for all parameters. It is concluded from our study that the use of poultry manure and spraying with urea contributes significantly to boosting the growth traits of Local lettuce, which is demonstrated positively in increasing the yield, so we recommend through the results of the study organic fertilisation with poultry manure and spraying with urea, as well as the interaction of the 2 study factors in order to give the best growth and yield to lettuce plants.

References

  1. 1. Yang X, Gil MI, Yang Q, Tomás-Barberán FA. Bioactive compounds in lettuce: highlighting the benefits to human health and impacts of preharvest and postharvest practices. Compr Rev Food Sci Food Saf. 2022;21(1):4–45. https://doi.org/10.1111/1541-4337.12877
  2. 2. Shi M, Gu J, Wu H, Rauf A, Emran TB, Khan Z, et al. Phytochemicals, nutrition, metabolism, bioavailability and health benefits in lettuce-A comprehensive review. Antioxidants. 2022;11(6):1158. https://doi.org/10.3390/antiox11061158
  3. 3. Mampholo BM, Maboko MM, Soundy P, Sivakumar D. Phytochemicals and overall quality of leafy lettuce (Lactuca sativa L.) varieties grown in closed hydroponic system. J Food Qual. 2016;39(6):805–15. https://doi.org/10.1111/jfq.12234
  4. 4. Zhao Q, Liu Y, Guo X. Performance analysis of two typical greenhouse lettuce production systems. Front Plant Sci. 2023;14:1165856. https://doi.org/10.3389/fpls.2023.1165856
  5. 5. Verma S, Pradhan SS, Singh A, Kushuwaha M. Effect of organic manure on different soil properties: a review. Int J Plant Soil Sci. 2024;36(5):182–7. https://doi.org/10.9734/ijpss/2024/v36i54515
  6. 6. Al-Dulaimy AFZ, Alfahdawi HE, Abdulhameed SH. Effect of humic acid application and CPPU spraying on growth and flowering of marigold. Ornam Hortic. 2025;31:e312965. https://doi.org/10.1590/2447-536x.v31.e312965
  7. 7. Alnuimi AYM, Al-Dulaimy AFZ. Response of fig seedlings cv. Aswad Diyala to biochar application and tryptophan foliar spray. Plant Sci Today. 2025;12(2):1–10.
  8. 8. Al-Jaf IHM, Alani MHI, Alalwani AK, Al-Dulaimy AFZ, Ahmed MM. Prospects of agriculture in Iraq in the confrontation of climate changes: a review. J Univ Anbar Pure Sci. 2025;19(1):105–14. https://doi.org/10.37652/juaps.2024.153040.1313
  9. 9. Kim MJ, Moon Y, Tou JC, Mou B, Waterland NL. Nutritional value, bioactive compounds and health benefits of lettuce (Lactuca sativa L.). J Food Compos Anal. 2016;49:19–34. https://doi.org/10.1016/j.jfca.2016.03.004
  10. 10. Saliga R, Skelly J. Using chicken manure safely in home gardens and landscapes (FS-13-23). University of Nevada Cooperative Extension; 2013. https://doi.org/10.1515/9780773590076-009
  11. 11. Barker AV, Pilbeam DJ. Handbook of plant nutrition. 2nd ed. CRC Press; 2015. https://doi.org/10.1201/b18458
  12. 12. Murad HJ, Al-Dulaimy AFZ. Response of date palms cv. Zahdi to foliar spray with urea and seaweed extract. IOP Conf Ser Earth Environ Sci. 2021;761(1):012052. https://doi.org/10.1088/1755-1315/761/1/012052
  13. 13. Abd El Galil A, Abou El-Salehein E, El Hamady M. Effect of foliar spray with urea and dry yeast extract on head lettuce production. J Product Dev. 2021;26(4):1071–86. https://doi.org/10.21608/jpd.2021.211864
  14. 14. Jahanbani S, Mumivand H, Zahedi B, Argento S. Foliar application of urea and amino acids regulates growth, photosynthesis, pigments, antioxidant activity and the essential oil content and composition of basil (Ocimum basilicum L.). Agronomy. 2024;14(12):2950. https://doi.org/10.3390/agronomy14122950
  15. 15. Yeshiwas Y, Zewdie BYB, Chekol A, Walle A. Effect of nitrogen fertilizer and farmyard manure on growth and yield of lettuce (Lactuca sativa L.). Int J Agric Res. 2018;13:74–9. https://doi.org/10.3923/ijar.2018.74.79
  16. 16. Al-Mehmedi S, Al-Mehmedi MFM. Statistics and experimental design. Amman: Dar Usama for Publishing and Distributing; 2012. p. 376.
  17. 17. Carter MR, Gregorich EG. Soil sampling and methods of analysis. 2nd ed. Boca Raton: CRC Press; 2007. https://doi.org/10.1201/9781420005271
  18. 18. Kaushik P, Pati PK, Khan ML, Khare PK. A quick and simple method for estimating leaf area by leaf weight. Int J Bot Stud. 2021;6(6):1286–8.
  19. 19. AOAC International. Official methods of analysis. 22nd ed. AOAC International; 2023.
  20. 20. Ogendo RO, Isutsa DK, Sigung DO. Interaction of farmyard manure and plant population density effects on soil characteristics and productivity of mulched strawberry in a tropical climate. Afr J Hortic Sci. 2008;1:100–15.
  21. 21. Al-Hayali REY, Al-Jaf IHM, Al Miahy FHR. Evaluation of orange and biofertilizer on some yield traits of Cucumber (Cucumis sativus L.). Bionatura. 2023;8(1):1–5. https://doi.org/10.21931/RB/CSS/S2023.08.01.46
  22. 22. Masarirambi MT. Effects of kraal manure, chicken manure and inorganic fertilizer on growth and yield of lettuce (Lactuca sativa L. var. Commander) in a semi-arid environment. Asian J. Agric. Sci. 2012; 4(1): 58–64.
  23. 23. Alani MHI, Sekhi YS, Almehemdi AF. Chemical analysis of Fig transplants cv. Wazeri under cutting type and humic acid. IOP Conf Ser Earth Environ Sci. 2021;761(1):012032. https://doi.org/10.1088/1755-1315/761/1/012032
  24. 24. Ahmed MS, Ismail MH, Abdullah AK, Al-Dulaimy AFZ. Influence of organic fertilizer and licorice root on growth, flowering and chemical constitution of Chinese carnation (Dianthus chinensis L.). Plant Sci Today. 2025;13(1):1–7.
  25. 25. Abed BM, Al-Jaf IHMS, Alalwani AK. Effect of potassium spraying and spraying dates on some growth, yield and chemical traits of tomato cv. Polonia. Plant Sci Today. 2025;12(4):1–7.
  26. 26. Alani MHI, Awad IM, Khudair RIA, Salim HA, Jasem SM. Response of Gazania plants (Gazania splendens L.) to seaweed extract (Tecamin Algae) and nano stimulator (Proteck CalBor). IOP Conf Ser Earth Environ Sci. 2021;910(1):012052. https://doi.org/10.1088/1755-1315/910/1/012052
  27. 27. Murad HJ. Effects of spraying with urea and seaweed extract (Tecamin Algae) on growth and chemical content of date palm tree cv. Zahdi. IOP Conf Ser Earth Environ Sci. 2021;904(1):012065. https://doi.org/10.1088/1755-1315/904/1/012065
  28. 28. Hamad YA, Sallume MO. The effect of spraying organic acids, nano and traditional iron on the growth and yield of mung bean (Vigna radiata L.). Int J Agric Stat Sci. 2021;17(2):831–6.
  29. 29. Nur D, Selcuk G, Yuksel T. Effect of organic manure application and solariziation of soil microbial biomass and enzyme activities under greenhouse conditions. Biol Agric Hortic. 2006;23:305–20. https://doi.org/10.1080/01448765.2006.9755331
  30. 30. Madisa ME, Mathowa T, Mpofu C, Stephen N, Machacha S. Effect of chicken manure and commercial fertilizer on performance of jute mallow (Corchorus olitorius). Agric Biol J N Am. 2013;4(6):617–22.
  31. 31. Marschner H. Marschner’s mineral nutrition of higher plants. London: Academic Press; 2012;89:651.
  32. 32. Havlin JL, Tisdale SL, Nelson WL, Beaton JD. Soil fertility and fertilizers: an introduction to nutrient management. 8th ed. Upper Saddle River: Prentice Hall; 2016.
  33. 33. Al-Dabbagh AMS, Zuhair AD. Effect of macnetic water and urea spray on yield of two lettuce cultivars (Regina dei Ghiacci and Great Lakes 118) in NFT culture system. Karbala J Agric Sci. 2018; Proceedings of the Third Agricultural Scientific Conference 5–6 March 2018. https://doi.org/10.59658/jkas.v5i5.606
  34. 34. Bošković-Rakočević L, Pavlović N, Mladenović J, Zdravković J, Tošić I, Marjanović M. Yield and quality of lettuce (Lactuca sativa L.) depending on variety and type of nitrogen fertilizer. Emir J Food Agric. 2023;35(8):715–21. https://doi.org/10.9755/ejfa.2023.3128
  35. 35. Alfahdawi HE, Al-Dulaimy AFZ. Effect of spraying with nano zinc and coconut milk on the growth and flowering of the petunia plant. Plant Sci Today. 2025;12(4):1–11.
  36. 36. Madina P, Ugoo TR, Adorolo MO. Variety of lettuce (Lactuca sativa L.) growth and yield in response to various organic fertilizers in Makurdi, Nigeria. Asian J Res Crop Sci. 2024;9(4):176–84. https://doi.org/10.9734/ajrcs/2024/v9i4309
  37. 37. Vella A, Sacco AT. The effect of poultry, cattle and swine manure applications to soil on lettuce yield and quality and groundwater contamination potential. Int J Agric Environ Res. 2022;8(2):194–208. https://doi.org/10.51193/IJAER.2022.8201
  38. 38. Altuntaş Ö, Küçük R, Ersoy L. The effect of chicken manure on plant growth, nutrient content and yield of lettuce. Biol Chem Res. 2022;9:9–18.

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